Detailed view for LmjF.34.0730

Basic information

TDR Targets ID: 29189
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.9459 | Length (AA): 210 | MW (Da): 22999 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF07228   Stage II sporulation protein E (SpoIIE)

Gene Ontology

Mouse over links to read term descriptions.
GO:0004721   phosphoprotein phosphatase activity  
GO:0003824   catalytic activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 189 1txo (A) 51 240 20.00 0 0.95 0.78 0.18
19 178 1a6q () 96 305 13.00 0 0.23 0.61 -0.2
16 152 2xzv (A) 73 228 18.00 0 0.65 0.724381 -0.32
20 187 2j82 (A) 1079 1240 20.00 0 0.94 0.844 0.25
85 200 4e7n (A) 120 245 34.00 0.96 0.31 0.538581 0.96

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127494)

Species Accession Gene Product
Arabidopsis thaliana AT2G30170   photosystem II core phosphatase
Arabidopsis thaliana AT4G16580   putative protein phosphatase 2C
Arabidopsis thaliana AT5G66720   putative protein phosphatase 2C 80
Brugia malayi Bm1_20765   5-azacytidine resistance protein azr1
Candida albicans CaO19.5661   similar to S. cerevisiae YHR076W
Candida albicans CaO19.13106   similar to S. cerevisiae YHR076W
Caenorhabditis elegans CELE_W09D10.4   Protein W09D10.4
Cryptosporidium hominis Chro.70512   hypothetical protein
Cryptosporidium parvum cgd7_4640   PP2C phosphatase
Dictyostelium discoideum DDB_G0280067   protein phosphatase 2C-related protein
Dictyostelium discoideum DDB_G0288107   protein phosphatase 2C-related protein
Drosophila melanogaster Dmel_CG7615   fos intronic gene
Drosophila melanogaster Dmel_CG15035   CG15035 gene product from transcript CG15035-RA
Drosophila melanogaster Dmel_CG12091   CG12091 gene product from transcript CG12091-RB
Echinococcus granulosus EgrG_000815300   protein phosphatase ptc7
Echinococcus multilocularis EmuJ_000815300   protein phosphatase ptc7
Homo sapiens 160760   PTC7 protein phosphatase homolog (S. cerevisiae)
Leishmania braziliensis LbrM.20.0660   hypothetical protein, conserved
Leishmania braziliensis LbrM.25.1620   hypothetical protein, conserved
Leishmania donovani LdBPK_340770.1   Stage II sporulation protein E (SpoIIE), putative
Leishmania donovani LdBPK_252140.1   Stage II sporulation protein E (SpoIIE), putative
Leishmania infantum LinJ.25.2140   hypothetical protein, conserved
Leishmania infantum LinJ.34.0770   hypothetical protein, conserved
Leishmania major LmjF.34.0730   hypothetical protein, conserved
Leishmania major LmjF.25.2060   hypothetical protein, conserved
Leishmania mexicana LmxM.33.0730   hypothetical protein, conserved
Leishmania mexicana LmxM.25.2060   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_03458   5-azacytidine resistance protein azr1
Mus musculus ENSMUSG00000038582   PTC7 protein phosphatase homolog (S. cerevisiae)
Oryza sativa 4334524   Os03g0809300
Onchocerca volvulus OVOC7807   Protein phosphatase PTC7 homolog
Plasmodium falciparum PF3D7_1009600   protein phosphatase, putative
Plasmodium knowlesi PKNH_0809300   protein phosphatase, putative
Plasmodium vivax PVX_094695   protein phosphatase 2C, putative
Saccharomyces cerevisiae YHR076W   type 2C protein phosphatase PTC7
Schistosoma japonicum Sjp_0085700   Protein phosphatase PTC7 homolog, putative
Schistosoma mansoni Smp_046890   protein phosphatase 2C
Schmidtea mediterranea mk4.008191.02   Protein phosphatase PTC7 homolog
Trypanosoma brucei gambiense Tbg972.4.3640   protein phosphatase 2C, putative
Trypanosoma brucei gambiense Tbg972.3.2100   protein phosphatase 2C, putative
Trypanosoma brucei Tb927.4.3680   protein phosphatase 2C, putative
Trypanosoma brucei Tb927.3.2150   protein phosphatase 2C, putative
Trypanosoma congolense TcIL3000_4_3180   protein phosphatase 2C, putative
Trypanosoma congolense TcIL3000_3_1260   protein phosphatase 2C, putative
Trypanosoma cruzi TcCLB.507081.50   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506201.120   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.504129.40   hypothetical protein, conserved
Trichomonas vaginalis TVAG_347460   protein phosphatase 2C, putative
Trichomonas vaginalis TVAG_158970   protein phosphatase 2C, putative

Essentiality

LmjF.34.0730 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.3680 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.3680 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.3680 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.3680 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.3.2150 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.2150 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.2150 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.3.2150 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier LmjF.34.0730 (Leishmania major), hypothetical protein, conserved
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