Detailed view for Smp_165900.2

Basic information

TDR Targets ID: 292706
Schistosoma mansoni, n2n2-dimethylguanosine tRNA methyltranserase related
Source Database / ID:  GeneDB

pI: 8.1112 | Length (AA): 416 | MW (Da): 45179 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02005   N2,N2-dimethylguanosine tRNA methyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0004809   tRNA (guanine-N2-)-methyltransferase activity  
GO:0003723   RNA binding  
GO:0008033   tRNA processing  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
24 390 3axs (A) 2 341 28.00 0 1 1.14991 -0.26
26 342 2dul (A) 6 293 33.00 0 1 0.988019 -0.11
75 191 3cbg (A) 52 171 25.00 0 0.71 0.63265 -0.71
80 190 1l3i (A) 30 134 22.00 0 0.98 0.577227 -0.84

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127349)

Species Accession Gene Product
Arabidopsis thaliana AT3G02320   putative N(2),N(2)-dimethylguanosine tRNA methyltransferase 2
Arabidopsis thaliana AT5G15810   N2,N2-dimethylguanosine tRNA methyltransferase
Babesia bovis BBOV_II005710   N(2),N(2)-dimethylguanosine tRNA methyltransferase, putative
Brugia malayi Bm1_16350   N(2),N(2)-dimethylguanosine tRNA methyltransferase, putative
Candida albicans CaO19.3265   likely N2,N2-dimethylguanosine-specific tRNA methyltransferase similar to S. cerevisiae TRM1 (YDR120C) mitochondrial tRNA (guani
Candida albicans CaO19.10775   likely N2,N2-dimethylguanosine-specific tRNA methyltransferase similar to S. cerevisiae TRM1 (YDR120C) mitochondrial tRNA (guani
Caenorhabditis elegans CELE_ZC376.5   Protein TRM-1
Cryptosporidium hominis Chro.40369   ENSANGP00000010185
Cryptosporidium parvum cgd4_3250   Trm1p. N2,N2-dimethylguanosine tRNA methyltransferase
Dictyostelium discoideum DDB_G0284575   hypothetical protein
Drosophila melanogaster Dmel_CG6388   CG6388 gene product from transcript CG6388-RA
Echinococcus granulosus EgrG_001059800   TRM1 tRNA methyltransferase 1
Entamoeba histolytica EHI_148760   N2,N2-dimethylguanosine tRNA methyltransferase, putative
Echinococcus multilocularis EmuJ_001059800   TRM1 tRNA methyltransferase 1
Giardia lamblia GL50803_12235   N2,N2-dimethylguanosine tRNA methyltransferase, putative
Homo sapiens ENSG00000104907   tRNA methyltransferase 1 homolog (S. cerevisiae)
Leishmania braziliensis LbrM.13.0750   N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative
Leishmania donovani LdBPK_130830.1   N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative
Leishmania infantum LinJ.13.0830   N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative
Leishmania major LmjF.13.0940   N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative
Leishmania mexicana LmxM.13.0940   N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative
Loa Loa (eye worm) LOAG_02318   hypothetical protein
Mus musculus ENSMUSG00000001909   tRNA methyltransferase 1
Neospora caninum NCLIV_038700   hypothetical protein
Oryza sativa 4348435   Os10g0357800
Oryza sativa 4334353   Os03g0786700
Plasmodium berghei PBANKA_1417800   tRNA (guanine(26)-N(2))-dimethyltransferase, putative
Plasmodium falciparum PF3D7_1319300   tRNA (guanine(26)-N(2))-dimethyltransferase, putative
Plasmodium knowlesi PKNH_1420100   tRNA (guanine(26)-N(2))-dimethyltransferase, putative
Plasmodium vivax PVX_122780   N(2),N(2)-dimethylguanosine tRNA methyltransferase, putative
Plasmodium yoelii PY07448   probable n(2),n(2)-dimethylguanosine tRNA methyltransferase-related
Plasmodium yoelii PY04503   N2,N2-dimethylguanosine tRNA methyltransferase
Saccharomyces cerevisiae YDR120C   Trm1p
Schistosoma japonicum Sjp_0001840   ko:K00555 tRNA (guanine-N2-)-methyltransferase [EC2.1.1.32], putative
Schistosoma mansoni Smp_165900.2   n2n2-dimethylguanosine tRNA methyltranserase related
Schmidtea mediterranea mk4.002285.01   N2,n2-dimethylguanosine tRNA methyltranserase related
Trypanosoma brucei gambiense Tbg972.11.4510   N(2), N(2)-dimethylguanosine tRNA methyltransferase
Trypanosoma brucei Tb927.11.3890   N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative
Trypanosoma brucei Tb427tmp.02.1390   N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative
Trypanosoma cruzi TcCLB.506733.60   N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative
Trypanosoma cruzi TcCLB.511857.40   N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative
Toxoplasma gondii TGME49_267480   tRNA (guanine(26)-N(2))-dimethyltransferase, putative
Theileria parva TP02_0525   N2,N2-dimethylguanosine tRNA methyltransferase, putative
Trichomonas vaginalis TVAG_353820   N(2),N(2)-dimethylguanosine tRNA methyltransferase, putative
Trichomonas vaginalis TVAG_309480   N(2),N(2)-dimethylguanosine tRNA methyltransferase, putative
Trichomonas vaginalis TVAG_309470   conserved hypothetical protein
Trichomonas vaginalis TVAG_439220   N(2),N(2)-dimethylguanosine tRNA methyltransferase, putative

Essentiality

Smp_165900.2 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.1390 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.1390 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.1390 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb11.02.1390 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1417800 Plasmodium berghei Slow plasmo
TGME49_267480 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier Smp_165900.2 (Schistosoma mansoni), n2n2-dimethylguanosine tRNA methyltranserase related
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