Detailed view for Smp_181240

Basic information

TDR Targets ID: 292743
Schistosoma mansoni, glutamyl-tRNA(gln) amidotransferase subunit B (mitochondrial and prokaryotic) pet112-related
Source Database / ID:  GeneDB

pI: 6.7658 | Length (AA): 472 | MW (Da): 52862 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02637   GatB domain
PF02934   GatB/GatE catalytic domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016884   carbon-nitrogen ligase activity, with glutamine as amido-N-donor  
GO:0016874   ligase activity  
GO:0003824   catalytic activity  
GO:0006412   translation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
33 460 3h0l (B) 3 412 28.00 0 1 1.17388 0.25
34 471 3al0 (B) 2 427 26.00 0 1 1.09107 0.99
35 322 4n0h (B) 27 317 40.00 0 0.93 0.849769 0.55
348 458 1upl (A) 184 291 16.00 0 0.08 0.375769 -0.29

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127330)

Species Accession Gene Product
Arabidopsis thaliana AT1G48520   Glutamyl-tRNA(Gln) amidotransferase subunit B
Babesia bovis BBOV_I001000   PET112 family, N terminal region domain containing protein
Brugia malayi Bm1_25000   hypothetical protein
Brugia malayi Bm1_44675   PET112 family, C terminal region containing protein
Brugia malayi Bm1_55815   PET112 family, N terminal region containing protein
Candida albicans CaO19.2494   similar to S. cerevisiae protein involved in mitochondrial translation
Candida albicans CaO19.10030   similar to S. cerevisiae protein involved in mitochondrial translation
Caenorhabditis elegans CELE_C39B5.6   Protein C39B5.6
Chlamydia trachomatis CT_004   aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Dictyostelium discoideum DDB_G0268776   glutamyl-tRNA amidotransferase B subunit
Drosophila melanogaster Dmel_CG5463   CG5463 gene product from transcript CG5463-RA
Echinococcus granulosus EgrG_000945100   glutamyl tRNA amidotransferase subunit B
Echinococcus multilocularis EmuJ_000945100   glutamyl tRNA amidotransferase subunit B
Homo sapiens ENSG00000059691   glutamyl-tRNA(Gln) amidotransferase, subunit B
Loa Loa (eye worm) LOAG_05469   hypothetical protein
Mycobacterium leprae ML1700c   PROBABLE GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE (SUBUNIT B) GATB (Glu-ADT SUBUNIT B)
Mus musculus ENSMUSG00000028085   glutamyl-tRNA(Gln) amidotransferase, subunit B
Mycobacterium tuberculosis Rv3009c   Probable glutamyl-tRNA(GLN) amidotransferase (subunit B) GatB (Glu-ADT subunit B)
Mycobacterium ulcerans MUL_1941   aspartyl/glutamyl-tRNA amidotransferase subunit B
Neospora caninum NCLIV_033440   hypothetical protein
Oryza sativa 4350686   Os11g0544800
Plasmodium berghei PBANKA_1127500   glutamyl-tRNA(Gln) amidotransferase subunit B, putative
Plasmodium falciparum PF3D7_0628800   glutamyl-tRNA(Gln) amidotransferase subunit B
Plasmodium knowlesi PKNH_1121000   glutamyl-tRNA(Gln) amidotransferase subunit B, putative
Plasmodium vivax PVX_114540   glutamyl-tRNA(Gln) amidotransferase subunit B, putative
Plasmodium yoelii PY03547   glutamyl-tRNA amidotransferase subunit b
Saccharomyces cerevisiae YBL080C   glutamyl-tRNA(Gln) amidotransferase subunit PET112
Schistosoma japonicum Sjp_0048040   ko:K02434 aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit B, putative
Schistosoma japonicum Sjp_0317240   IPR004413,Glutamyl-tRNA(Gln) amidotransferase B subunit,domain-containing
Schistosoma mansoni Smp_181240   glutamyl-tRNA(gln) amidotransferase subunit B (mitochondrial and prokaryotic) pet112-related
Schmidtea mediterranea mk4.007649.01  
Schmidtea mediterranea mk4.018486.00  
Toxoplasma gondii TGME49_233838   PET112 family, C terminal region domain-containing protein
Treponema pallidum TP1021   aspartyl/glutamyl-tRNA amidotransferase subunit B
Theileria parva TP01_0031   glutamyl-tRNA amidotransferase subunit B, putative
Wolbachia endosymbiont of Brugia malayi Wbm0704   aspartyl/glutamyl-tRNA amidotransferase subunit B

Essentiality

Smp_181240 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu3060 Mycobacterium tuberculosis essential nmpdr
PBANKA_1127500 Plasmodium berghei Essential plasmo
TGME49_233838 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Smp_181240 (Schistosoma mansoni), glutamyl-tRNA(gln) amidotransferase subunit B (mitochondrial and prokaryotic) pet112-related
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