Detailed view for LmjF.34.0110

Basic information

TDR Targets ID: 29344
Leishmania major, adenylate kinase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.1888 | Length (AA): 218 | MW (Da): 24404 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00406   Adenylate kinase
PF05191   Adenylate kinase, active site lid

Gene Ontology

Mouse over links to read term descriptions.
GO:0019205   nucleobase, nucleoside, nucleotide kinase activity  
GO:0016776   phosphotransferase activity, phosphate group as acceptor  
GO:0006139   nucleobase, nucleoside, nucleotide and nucleic acid metabolic process  
GO:0019201   nucleotide kinase activity  
GO:0005524   ATP binding  
GO:0004017   adenylate kinase activity  

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 218 2bbw (A) 6 220 33.00 0 1 1.43 -0.74
1 209 1e4v (A) 1 214 49.00 0 1 1.61 -1.25
1 212 2ak3 (A) 6 214 45.00 0 1 1.61 -1.12
1 208 4jzk (A) 1 213 50.00 0 1 1.67443 -1.49
1 217 1zak (A) 6 217 35.00 0.00000062 1 1.45331 -0.57

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_126762)

Species Accession Gene Product
Arabidopsis thaliana AT5G63400   adenylate kinase 1
Arabidopsis thaliana AT5G50370   adenylate kinase 2
Arabidopsis thaliana AT5G47840   adenosine monophosphate kinase
Babesia bovis BBOV_IV002930   adenylate kinase 2
Brugia malayi Bm1_24575   adenylate kinase isoenzyme 1
Candida albicans CaO19.10894   one of two adenylate kinase genes similar to S. cerevisiae ADK1 (YDR226W)
Candida albicans CaO19.683   one of two adenylate kinase genes similar to S. cerevisiae ADK1 (YDR226W)
Candida albicans CaO19.3391   one of two adenylate kinase genes similar to S. cerevisiae ADK1 (YDR226W)
Candida albicans CaO19.8301   one of two adenylate kinase genes similar to S. cerevisiae ADK1 (YDR226W)
Caenorhabditis elegans CELE_C29E4.8   Protein LET-754
Cryptosporidium hominis Chro.50037   type 2 adenylate kinase
Cryptosporidium parvum cgd5_3360   adenylate kinase
Chlamydia trachomatis CT_128   adenylate kinase
Dictyostelium discoideum DDB_G0283805   adenylate kinase
Drosophila melanogaster Dmel_CG3140   Adenylate kinase-2
Escherichia coli b0474   adenylate kinase
Echinococcus granulosus EgrG_000229900   adenylate kinase
Echinococcus granulosus EgrG_000421600   adenylate kinase 2 mitochondrial
Echinococcus granulosus EgrG_000230000   f box and leucine rich repeat protein
Entamoeba histolytica EHI_135470   adenylate kinase
Echinococcus multilocularis EmuJ_000230000   f box and leucine rich repeat protein
Echinococcus multilocularis EmuJ_000421700   adenylate kinase 2, mitochondrial
Echinococcus multilocularis EmuJ_000229900   adenylate kinase
Giardia lamblia GL50803_28234   Adenylate kinase
Homo sapiens ENSG00000165695   adenylate kinase 8
Homo sapiens ENSG00000004455   adenylate kinase 2
Leishmania braziliensis LbrM.21.1450   adenylate kinase, putative
Leishmania braziliensis LbrM.20.0060   adenylate kinase, putative
Leishmania donovani LdBPK_340120.1   adenylate kinase, putative
Leishmania donovani LdBPK_211490.1   adenylate kinase, putative
Leishmania infantum LinJ.34.0120   adenylate kinase, putative
Leishmania infantum LinJ.21.1490   adenylate kinase, putative
Leishmania major LmjF.34.0110   adenylate kinase, putative
Leishmania major LmjF.21.1250   adenylate kinase, putative
Leishmania mexicana LmxM.21.1250   adenylate kinase, putative
Leishmania mexicana LmxM.33.0110   adenylate kinase, putative
Loa Loa (eye worm) LOAG_15923   hypothetical protein
Loa Loa (eye worm) LOAG_01018   hypothetical protein
Loa Loa (eye worm) LOAG_14551   hypothetical protein
Mycobacterium leprae ML1832c   PROBABLE ADENYLATE KINASE KAD (ATP-AMP TRANSPHOSPHORYLASE)
Mus musculus ENSMUSG00000026807   adenylate kinase 8
Mus musculus ENSMUSG00000028792   adenylate kinase 2
Mycobacterium tuberculosis Rv0733   Adenylate kinase Adk (ATP-AMP transphosphorylase)
Mycobacterium ulcerans MUL_0829   adenylate kinase
Neospora caninum NCLIV_047810   hypothetical protein
Oryza sativa 4351850   Os12g0236400
Oryza sativa 4350358   Os11g0312400
Oryza sativa 4344470   Os08g0109300
Onchocerca volvulus OVOC12241   Adenylate kinase isoenzyme 1 homolog
Plasmodium berghei PBANKA_1207200   adenylate kinase, putative
Plasmodium falciparum PF3D7_1008900   adenylate kinase
Plasmodium knowlesi PKNH_0808600   adenylate kinase, putative
Plasmodium vivax PVX_094660   adenylate kinase 2, putative
Plasmodium yoelii PY01562   adenylate kinase b
Saccharomyces cerevisiae YDR226W   adenylate kinase ADK1
Schistosoma japonicum Sjp_0305220   ko:K00939 adenylate kinase [EC2.7.4.3], putative
Schistosoma mansoni Smp_061940.2   adenylate kinase
Schistosoma mansoni Smp_061940.3   adenylate kinase
Schmidtea mediterranea mk4.021270.00   Adenylate kinase
Schmidtea mediterranea mk4.031757.00   Adenylate kinase
Schmidtea mediterranea mk4.017984.00   Adenylate kinase
Schmidtea mediterranea mk4.003479.00   Adenylate kinase
Schmidtea mediterranea mk4.003072.06   Adenylate kinase
Schmidtea mediterranea mk4.004522.06   Adenylate kinase
Schmidtea mediterranea mk4.003072.08   Adenylate kinase
Schmidtea mediterranea mk4.000084.17   Adenylate kinase 8
Trypanosoma brucei gambiense Tbg972.10.880   adenylate kinase, putative
Trypanosoma brucei gambiense Tbg972.10.3150   adenylate kinase, putative
Trypanosoma brucei Tb927.10.2530   adenylate kinase, putative
Trypanosoma brucei Tb927.10.830   adenylate kinase, putative
Trypanosoma congolense TcIL3000_10_2160   adenylate kinase, putative
Trypanosoma congolense TcIL3000_10_680   adenylate kinase, putative
Trypanosoma cruzi TcCLB.503479.30   adenylate kinase 1
Trypanosoma cruzi TcCLB.506855.180   adenylate kinase, putative
Trypanosoma cruzi TcCLB.507883.80   adenylate kinase, putative
Trypanosoma cruzi TcCLB.506195.80   adenylate kinase, putative
Toxoplasma gondii TGME49_307603   adenylate kinase
Toxoplasma gondii TGME49_224900   adenylate kinase, putative
Treponema pallidum TP0595   adenylate kinase (adk)
Theileria parva TP01_0633   adenylate kinase, putative
Trichomonas vaginalis TVAG_489800   NADH dehydrogenase 51 kDa subunit, putative
Trichomonas vaginalis TVAG_072700   NADH dehydrogenase 51 kDa subunit, putative
Wolbachia endosymbiont of Brugia malayi Wbm0321   adenylate kinase

Essentiality

LmjF.34.0110 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.2530 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.2530 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.2530 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.2530 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.830 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.830 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.830 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.830 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b0474 Escherichia coli essential goodall
CELE_C29E4.8 Caenorhabditis elegans embryonic lethal wormbase
CELE_C29E4.8 Caenorhabditis elegans larval arrest wormbase
CELE_C29E4.8 Caenorhabditis elegans larval lethal wormbase
CELE_C29E4.8 Caenorhabditis elegans slow growth wormbase
CELE_C29E4.8 Caenorhabditis elegans sterile wormbase
YDR226W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1207200 Plasmodium berghei Essential plasmo
TGME49_224900 Toxoplasma gondii Probably essential sidik
TGME49_307603 Toxoplasma gondii Probably essential sidik
TGME49_224900 Toxoplasma gondii Essentiality uncertain sidik
TGME49_307603 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.8

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Rattus norvegicus Adenylate kinase 2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.0038 1 1

Assayability

Assay information

  • Assay for Myokinase (2.7.4.3 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Leishmania donovani ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    LmjF.34.0110 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Leishmania donovani ( 1 )

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier LmjF.34.0110 (Leishmania major), adenylate kinase, putative
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