Detailed view for LmjF.36.4190

Basic information

TDR Targets ID: 29443
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.0278 | Length (AA): 343 | MW (Da): 36608 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF05916   GINS complex protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0005634   nucleus  
GO:0006260   DNA replication  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
36 225 2e9x (B) 2 175 28.00 0 1 0.885836 -0.58
117 150 2kh2 (B) 89 122 53.00 0.27 0.04 0.461325 1.62

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128484)

Species Accession Gene Product
Arabidopsis thaliana AT3G12530   DNA replication complex GINS protein PSF2
Brugia malayi Bm1_28470   Probable DNA replication complex GINS protein PSF2, putative
Candida albicans CaO19.57   similar to C terminus of S. cerevisiae PSF2 (YJL072C) component of the GINS complex involved in DNA replication initiation
Candida albicans CaO19.7718   similar to C terminus of S. cerevisiae PSF2 (YJL072C) component of the GINS complex involved in DNA replication initiation
Caenorhabditis elegans CELE_F31C3.5   Protein F31C3.5
Cryptosporidium parvum cgd1_3410   DNA replication complex GINS protein PSF2, putative
Dictyostelium discoideum DDB_G0293564   GINS complex subunit 2
Drosophila melanogaster Dmel_CG18013   CG18013 gene product from transcript CG18013-RC
Echinococcus granulosus EgrG_000795300   dna replication complex gins protein psf2
Entamoeba histolytica EHI_069340   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000795300   dna replication complex gins protein psf2
Homo sapiens 51659   GINS complex subunit 2 (Psf2 homolog)
Leishmania braziliensis LbrM.35.4440   hypothetical protein, conserved
Leishmania donovani LdBPK_364400.1   GINS complex protein, putative
Leishmania infantum LinJ.36.4400   hypothetical protein, conserved
Leishmania major LmjF.36.4190   hypothetical protein, conserved
Leishmania mexicana LmxM.36.4190   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_12803   hypothetical protein
Loa Loa (eye worm) LOAG_14962   hypothetical protein
Mus musculus 272551   GINS complex subunit 2 (Psf2 homolog)
Oryza sativa 4327215   Os01g0248600
Saccharomyces cerevisiae YJL072C   Psf2p
Schistosoma japonicum Sjp_0001940   ko:K10733 DNA replication complex GINS protein PSF2, putative
Schistosoma mansoni Smp_100380   hypothetical protein
Schmidtea mediterranea mk4.004320.01  
Trypanosoma brucei gambiense Tbg972.11.11730   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.10460   dna replication complex gins protein psf2
Trypanosoma congolense TcIL3000.11.11020   GINS complex protein, putative
Trypanosoma cruzi TcCLB.510283.150   dna replication complex gins protein psf2
Trypanosoma cruzi TcCLB.503823.110   dna replication complex gins protein psf2
Trichomonas vaginalis TVAG_174630   conserved hypothetical protein

Essentiality

LmjF.36.4190 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.2230 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.2230 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.2230 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.2230 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F31C3.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_F31C3.5 Caenorhabditis elegans larval arrest wormbase
YJL072C Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier LmjF.36.4190 (Leishmania major), hypothetical protein, conserved
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