Detailed view for Smp_172420

Basic information

TDR Targets ID: 295695
Schistosoma mansoni, ubiquitin protein ligase E3a
Source Database / ID:  GeneDB

pI: 8.5387 | Length (AA): 3043 | MW (Da): 337095 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00632   HECT-domain (ubiquitin-transferase)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0016881   acid-amino acid ligase activity  
GO:0005488   binding  
GO:0004842   ubiquitin-protein ligase activity  
GO:0006464   protein modification process  

Metabolic Pathways

Ubiquitin mediated proteolysis (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
105 1100 1u6g (C) 12 1069 10.00 0 0.97 0.286909 0.71
662 998 4rxh (B) 177 507 12.00 0.000093 0.39 0.221846 -0.64
2625 3035 3g1n (B) 3996 4366 31.00 0 1 0.342364 0.16
2851 3037 5hps (A) 735 917 32.00 0 1 0.355053 0.12
2940 3033 3pt3 (A) 2703 2791 34.00 0 0.9 0.230491 -0.82
2949 3033 3pt3 (A) 2712 2791 40.00 0.079 0.86 0.304233 -0.57

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128439)

Species Accession Gene Product
Arabidopsis thaliana AT5G02880   E3 ubiquitin-protein ligase UPL4
Arabidopsis thaliana AT4G38600   HECT ubiquitin protein ligase family protein
Babesia bovis BBOV_IV000570   ubiquitin transferase, HECT domain containing protein
Brugia malayi Bm1_16175   thyroid receptor interacting protein 12
Candida albicans CaO19.3237   similar to S. cerevisiae UFD4 (YKL010C) ubiquitin--protein ligase
Candida albicans CaO19.10747   similar to S. cerevisiae UFD4 (YKL010C) ubiquitin--protein ligase
Dictyostelium discoideum DDB_G0287221   armadillo repeat-containing protein
Drosophila melanogaster Dmel_CG42574   circadian trip
Echinococcus granulosus EgrG_000106000   E3 ubiquitin protein ligase TRIP12
Echinococcus multilocularis EmuJ_000106000   E3 ubiquitin protein ligase TRIP12
Homo sapiens ENSG00000153827   thyroid hormone receptor interactor 12
Leishmania braziliensis LbrM.32.1190   hypothetical protein, conserved
Leishmania donovani LdBPK_321150.1   HECT-domain (ubiquitin-transferase), putative
Leishmania infantum LinJ.32.1150   hypothetical protein, conserved
Leishmania major LmjF.32.1090   hypothetical protein, conserved
Leishmania mexicana LmxM.31.1090   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_05367   hypothetical protein
Loa Loa (eye worm) LOAG_14322   hypothetical protein
Loa Loa (eye worm) LOAG_11488   thyroid hormone receptor interactor 12
Mus musculus ENSMUSG00000026219   thyroid hormone receptor interactor 12
Oryza sativa 4339037   Os05g0463900
Oryza sativa 4327983   Os02g0101700
Oryza sativa 4337658   Os05g0122400
Saccharomyces cerevisiae YKL010C   putative ubiquitin-protein ligase UFD4
Schistosoma japonicum Sjp_0131350   expressed protein
Schistosoma japonicum Sjp_0063470   E3 ubiquitin-protein ligase UPL3, putative
Schistosoma mansoni Smp_172420   ubiquitin protein ligase E3a
Schmidtea mediterranea mk4.004160.03  
Schmidtea mediterranea mk4.000217.04  
Schmidtea mediterranea mk4.016586.01  
Schmidtea mediterranea mk4.014585.00  
Schmidtea mediterranea mk4.000514.00  
Trypanosoma brucei gambiense Tbg972.11.16050   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.14330   HECT-domain (ubiquitin-transferase), putative
Trypanosoma congolense TcIL3000.11.14630   Zinc finger, ZZ type, putative
Trypanosoma cruzi TcCLB.511725.160   HECT-domain (ubiquitin-transferase), putative
Theileria parva TP01_0078   hypothetical protein
Trichomonas vaginalis TVAG_056400   hect E3 ubiquitin ligase, putative
Trichomonas vaginalis TVAG_248540   ubiquitin protein ligase E3a, putative
Trichomonas vaginalis TVAG_201990   ubiquitin protein ligase E3a, putative
Trichomonas vaginalis TVAG_269910   ubiquitin protein ligase E3a, putative
Trichomonas vaginalis TVAG_248520   conserved hypothetical protein
Trichomonas vaginalis TVAG_119130   ubiquitin protein ligase E3a, putative
Trichomonas vaginalis TVAG_063800   ubiquitin protein ligase E3a, putative

Essentiality

Smp_172420 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.5930 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.5930 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.5930 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.5930 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Smp_172420 (Schistosoma mansoni), ubiquitin protein ligase E3a
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