Detailed view for Smp_173320

Basic information

TDR Targets ID: 295891
Schistosoma mansoni, tubulin tyrosine ligase
Source Database / ID:  GeneDB

pI: 5.1662 | Length (AA): 553 | MW (Da): 65646 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03133   Tubulin-tyrosine ligase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0015643   toxin binding  
GO:0005524   ATP binding  
GO:0004835   tubulin-tyrosine ligase activity  
GO:0030153   bacteriocin immunity  
GO:0006464   protein modification process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
180 543 4ihj (F) 2 371 20.00 0 1 0.639328 0.26
194 406 4ylr (A) 55 234 32.00 0.1 0.24 0.184772 2.26
276 520 4i4t (F) 90 338 27.00 0.0024 1 0.649638 0.05
302 518 5vlq (B) 313 525 32.00 0.00014 0.99 0.290005 1.43

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_131073)

Species Accession Gene Product
Arabidopsis thaliana AT1G77550   tubulin-tyrosine ligase
Brugia malayi Bm1_14455   protein KIAA0153
Caenorhabditis elegans CELE_D2013.9   Protein TTLL-12
Drosophila melanogaster Dmel_CG1550   CG1550 gene product from transcript CG1550-RA
Echinococcus granulosus EgrG_000041800   tubulin tyrosine ligase protein 12
Echinococcus granulosus EgrG_00040300   tubulin tyrosine ligase protein 12
Entamoeba histolytica EHI_076890   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000040300   tubulin tyrosine ligase protein 12
Echinococcus multilocularis EmuJ_000041800   tubulin tyrosine ligase protein 12
Homo sapiens ENSG00000100304   tubulin tyrosine ligase-like family, member 12
Loa Loa (eye worm) LOAG_08031   hypothetical protein
Mus musculus ENSMUSG00000016757   tubulin tyrosine ligase-like family, member 12
Oryza sativa 4331821   Os03g0179000
Schistosoma japonicum Sjp_0025750   ko:K06047 Tubulin-tyrosine ligase [EC:6.3.2.25], putative
Schistosoma mansoni Smp_173320   tubulin tyrosine ligase
Schmidtea mediterranea mk4.002185.00   TubulinALQ-tyrosine ligase-like protein 12
Trypanosoma brucei gambiense Tbg972.11.2670   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.2390   Tubulin-tyrosine ligase family, putative
Trypanosoma brucei Tb927.11.2420   Tubulin-tyrosine ligase family, putative
Trypanosoma congolense TcIL3000.11.2220   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.504153.70   Tubulin-tyrosine ligase family, putative
Trichomonas vaginalis TVAG_146480   hypothetical protein
Trichomonas vaginalis TVAG_178680   hypothetical protein

Essentiality

Smp_173320 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.0020 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.0020 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.0020 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.0020 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.22.0002 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.22.0002 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.22.0002 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.22.0002 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Smp_173320 (Schistosoma mansoni), tubulin tyrosine ligase
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