Detailed view for LmjF.36.3740

Basic information

TDR Targets ID: 29840
Leishmania major, 60S ribosomal protein L34, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 12.3131 | Length (AA): 168 | MW (Da): 19266 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01199   Ribosomal protein L34e

Gene Ontology

Mouse over links to read term descriptions.
GO:0005840   ribosome  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0006412   translation  

Metabolic Pathways

Ribosome (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
50 153 4kzy (G) 117 231 28.00 0.6 0.03 0.543548 1.43
98 155 1w9r (A) 51 110 24.00 0.14 0.01 0.563138 -0.13

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127025)

Species Accession Gene Product
Arabidopsis thaliana AT3G28900   60S ribosomal protein L34-3
Arabidopsis thaliana AT1G26880   60S ribosomal protein L34-1
Arabidopsis thaliana AT1G69620   60S ribosomal protein L34-2
Babesia bovis BBOV_IV001680   ribosomal protein L34, putative
Brugia malayi Bm1_29260   60S ribosomal protein L34
Caenorhabditis elegans CELE_C42C1.14   Protein RPL-34
Cryptosporidium hominis Chro.80400   hypothetical protein
Cryptosporidium parvum cgd8_3480   60S ribosomal protein L34
Dictyostelium discoideum DDB_G0286389   S60 ribosomal protein L34
Drosophila melanogaster Dmel_CG9354   Ribosomal protein L34b
Drosophila melanogaster Dmel_CG6090   Ribosomal protein L34a
Entamoeba histolytica EHI_189940   60S ribosomal protein L34
Entamoeba histolytica EHI_167050   60S ribosomal protein L34
Entamoeba histolytica EHI_164870   60S ribosomal protein L34
Giardia lamblia GL50803_36069   Ribosomal protein L34
Homo sapiens ENSG00000109475   ribosomal protein L34
Leishmania braziliensis LbrM.35.3980   60S ribosomal protein L34, putative
Leishmania donovani LdBPK_363930.1   60S ribosomal protein L34, putative
Leishmania infantum LinJ.36.3930   60S ribosomal protein L34, putative
Leishmania infantum LinJ.18.1380   60S ribosomal protein L34, putative
Leishmania major LmjF.36.3740   60S ribosomal protein L34, putative
Leishmania major LmjF.18.1400   60S ribosomal protein L34, putative
Leishmania mexicana LmxM.18.1400   60S ribosomal protein L34, putative
Leishmania mexicana LmxM.36.3740   60S ribosomal protein L34, putative
Loa Loa (eye worm) LOAG_05257   60S ribosomal protein L34
Mus musculus 623174   predicted gene 6404
Mus musculus 100043876   predicted gene 4705
Mus musculus ENSMUSG00000062006   ribosomal protein L34
Neospora caninum NCLIV_045670   hypothetical protein
Oryza sativa 4345669   Os08g0436800
Oryza sativa 9267671   Os09g0413500
Oryza sativa 4344696   Os08g0156800
Plasmodium berghei PBANKA_1221200   60S ribosomal protein L34, putative
Plasmodium falciparum PF3D7_0710600   60S ribosomal protein L34
Plasmodium knowlesi PKNH_0109300   60S ribosomal protein L34, putative
Plasmodium vivax PVX_088065   60S ribosomal protein L34, putative
Plasmodium yoelii PY04861   Ribosomal protein L34e, putative
Saccharomyces cerevisiae YER056C-A   ribosomal 60S subunit protein L34A
Saccharomyces cerevisiae YIL052C   ribosomal 60S subunit protein L34B
Schmidtea mediterranea mk4.000821.06   60S ribosomal protein L34
Trypanosoma brucei gambiense Tbg972.10.15290   60S ribosomal protein L34, putative
Trypanosoma brucei gambiense Tbg972.11.10920   60S ribosomal protein L34, putative
Trypanosoma brucei Tb927.10.12680   60S ribosomal protein L34, putative
Trypanosoma congolense TcIL3000_10_10790   60S ribosomal protein L34, putative
Trypanosoma congolense TcIL3000.11.10210   60S ribosomal protein L34, putative
Trypanosoma cruzi TcCLB.507831.90   60S ribosomal protein L34, putative
Trypanosoma cruzi TcCLB.506963.20   60S ribosomal protein L34, putative
Trypanosoma cruzi TcCLB.511261.140   60S ribosomal protein L34, putative
Trypanosoma cruzi TcCLB.511233.20   60S ribosomal protein L34, putative
Toxoplasma gondii TGME49_227600   ribosomal protein RPL34
Theileria parva TP03_0488   60S ribosomal protein L34a, putative
Trichomonas vaginalis TVAG_359800   60S ribosomal protein L34, putative
Trichomonas vaginalis TVAG_094720   60S ribosomal protein L34, putative
Trichomonas vaginalis TVAG_417810   60S ribosomal protein L34, putative
Trichomonas vaginalis TVAG_094730   60S ribosomal protein L34, putative

Essentiality

LmjF.36.3740 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.12680 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.12680 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.12680 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.12680 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C42C1.14 Caenorhabditis elegans embryonic lethal wormbase
CELE_C42C1.14 Caenorhabditis elegans larval arrest wormbase
CELE_C42C1.14 Caenorhabditis elegans slow growth wormbase
CELE_C42C1.14 Caenorhabditis elegans sterile wormbase
TGME49_227600 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.36.3740 (Leishmania major), 60S ribosomal protein L34, putative
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