Detailed view for TcCLB.511751.70

Basic information

TDR Targets ID: 30036
Trypanosoma cruzi, microtubule associated protein xmap215
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.6817 | Length (AA): 1625 | MW (Da): 181325 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0005488   binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 11 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
28 975 1u6g (C) 11 1209 10.00 0 1 0.28 0.24
295 512 2db0 (A) 40 246 11.00 0.00000000031 0.23 0.32 -1.36
28 636 3grl (A) 27 588 13.00 0 1 0.357069 0.63
132 233 4g3a (A) 126 230 26.00 0.54 1 0.508969 -1.15
278 499 2qk2 (A) 271 490 24.00 0.0000000017 1 0.587815 -1.5
278 506 2qk2 (A) 271 497 19.00 0 1 0.462223 -1.11
301 517 5dn7 (A) 359 582 12.00 0 1 0.381838 -0.96
323 1137 2x1g (F) 68 937 12.00 0 1 0.550838 0.36
371 1502 1u6g (C) 7 1194 13.00 0 1 0.707915 0.64
610 824 4y5j (A) 601 812 21.00 0.000062 1 0.550508 -1.47
981 1083 3fmf (D) 100 195 36.00 0.4 0.05 0.196585 0.73

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_128564)

Species Accession Gene Product
Arabidopsis thaliana AT2G35630   protein MICROTUBULE ORGANIZATION 1
Brugia malayi Bm1_06160   zyg-9 protein
Candida albicans CaO19.6610   microtubule nucleation
Caenorhabditis elegans CELE_F22B5.7   Protein ZYG-9
Dictyostelium discoideum DDB_G0268616   XMAP215 family protein
Drosophila melanogaster Dmel_CG5000   mini spindles
Echinococcus granulosus EgrG_000794000   cytoskeleton associated protein 5
Echinococcus granulosus EgrG_000249200   microtubule associated protein xmap215
Entamoeba histolytica EHI_118620   HEAT repeat domain containing protein
Echinococcus multilocularis EmuJ_000794000   cytoskeleton associated protein 5
Echinococcus multilocularis EmuJ_000249200   microtubule associated protein xmap215
Homo sapiens ENSG00000175216   cytoskeleton associated protein 5
Leishmania braziliensis LbrM.30.1800   hypothetical protein, conserved
Leishmania donovani LdBPK_301760.1   microtubule associated protein xmap215
Leishmania infantum LinJ.30.1760   hypothetical protein, conserved
Leishmania major LmjF.30.1760   hypothetical protein, conserved
Leishmania mexicana LmxM.29.1760   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_05956   hypothetical protein
Loa Loa (eye worm) LOAG_15272   hypothetical protein
Loa Loa (eye worm) LOAG_14025   hypothetical protein
Loa Loa (eye worm) LOAG_06569   hypothetical protein
Mus musculus ENSMUSG00000040549   cytoskeleton associated protein 5
Oryza sativa 9268306   Os01g0816500
Oryza sativa 4327486   Os01g0816400
Saccharomyces cerevisiae YLR045C   Stu2p
Schistosoma japonicum Sjp_0061790   Cytoskeleton-associated protein 5, putative
Schistosoma japonicum Sjp_0313170   Cytoskeleton-associated protein 5, putative
Schistosoma japonicum Sjp_0081720   Cytoskeleton-associated protein 5, putative
Schistosoma mansoni Smp_054790.1   microtubule associated protein xmap215
Schistosoma mansoni Smp_054790.2   microtubule associated protein xmap215
Schmidtea mediterranea mk4.020633.00  
Schmidtea mediterranea mk4.005916.00  
Schmidtea mediterranea mk4.011435.00  
Schmidtea mediterranea mk4.018397.00   Zygote defective protein 9
Schmidtea mediterranea mk4.023251.01   Cytoskeleton-associated protein 5
Schmidtea mediterranea mk4.019504.00  
Schmidtea mediterranea mk4.010187.01  
Schmidtea mediterranea mk4.010187.02   Cytoskeleton-associated protein 5
Schmidtea mediterranea mk4.038801.01  
Trypanosoma brucei gambiense Tbg972.6.2880   hypothetical protein, conserved
Trypanosoma brucei Tb927.6.3090   microtubule associated protein xmap215
Trypanosoma congolense TcIL3000_6_2620   microtubule associated protein xmap215
Trypanosoma congolense TcIL3000_0_50920   cytoskeleton-associated protein, putative
Trypanosoma cruzi TcCLB.507023.160   microtubule associated protein xmap215
Trypanosoma cruzi TcCLB.511751.70   microtubule associated protein xmap215

Essentiality

TcCLB.511751.70 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.3090 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.6.3090 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.3090 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.3090 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F22B5.7 Caenorhabditis elegans embryonic lethal wormbase
YLR045C Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TcCLB.511751.70 (Trypanosoma cruzi), microtubule associated protein xmap215
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