Detailed view for TcCLB.510531.50

Basic information

TDR Targets ID: 30057
Trypanosoma cruzi, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.9912 | Length (AA): 755 | MW (Da): 82544 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 10

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF07690   Major Facilitator Superfamily

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  
GO:0055085   transmembrane transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
32 492 1pw4 (A) 6 447 13.00 0 0.2 0.67 -1.04
48 498 4gc0 (A) 6 479 14.00 0 0.9 0.762151 -0.28
287 467 4tph (A) 9 189 24.00 0.32 0.6 0.379835 0.18
346 484 4d2b (A) 74 202 29.00 0.32 0.3 0.230206 1.42

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_132015)

Species Accession Gene Product
Babesia bovis BBOV_III003420   transporter, major facilitator family
Cryptosporidium hominis Chro.70384   hypothetical protein
Cryptosporidium hominis Chro.20159   transporter
Cryptosporidium parvum cgd7_3450   major facilitator (MFS) superfamily transporter containing 12 transmembrane domains
Cryptosporidium parvum cgd2_1450   major facilitator superfamily protein, putative, 12 transmembrane domain
Leishmania braziliensis LbrM.22.0950   hypothetical protein, conserved
Leishmania donovani LdBPK_220870.1   major facilitator superfamily, putative
Leishmania infantum LinJ.22.0870   hypothetical protein, conserved
Leishmania major LmjF.22.1050   hypothetical protein, conserved
Leishmania mexicana LmxM.22.1050   hypothetical protein, conserved
Neospora caninum NCLIV_027810   hypothetical protein, conserved
Neospora caninum NCLIV_008080   hypothetical protein
Plasmodium berghei PBANKA_0817000   major facilitator superfamily domain-containing protein, putative
Plasmodium falciparum PF3D7_0916000   major facilitator superfamily domain-containing protein, putative
Plasmodium knowlesi PKNH_0714000   major facilitator superfamily domain-containing protein, putative
Plasmodium vivax PVX_099230   sugar transporter, putative
Plasmodium yoelii PY00954   major facilitator superfamily protein, putative
Trypanosoma brucei gambiense Tbg972.7.3430   transporter protein, putative
Trypanosoma brucei Tb927.7.3110   transporter protein, putative
Trypanosoma cruzi TcCLB.511819.10   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510531.50   hypothetical protein, conserved
Toxoplasma gondii TGME49_253700   transporter, major facilitator family protein
Toxoplasma gondii TGME49_258700   transporter, major facilitator family protein
Theileria parva TP03_0342   hypothetical protein
Theileria parva TP03_0894   hypothetical protein, conserved
Theileria parva TP03_0340   hypothetical protein
Theileria parva TP03_0338   hypothetical protein
Theileria parva TP03_0341   hypothetical protein
Theileria parva TP03_0339   hypothetical protein
Theileria parva TP03_0337   hypothetical protein

Essentiality

TcCLB.510531.50 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.3110 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.3110 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.3110 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.3110 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0817000 Plasmodium berghei Dispensable plasmo
TGME49_253700 Toxoplasma gondii Essentiality uncertain sidik
TGME49_258700 Toxoplasma gondii Essentiality uncertain sidik
TGME49_253700 Toxoplasma gondii Probably essential sidik
TGME49_258700 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Staphylococcus aureus Quinolone resistance protein norA 388 aa 21.4% 393 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.510531.50 (Trypanosoma cruzi), hypothetical protein, conserved
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