Detailed view for TcCLB.508173.150

Basic information

TDR Targets ID: 30497
Trypanosoma cruzi, protein disulfide isomerase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 10.1033 | Length (AA): 141 | MW (Da): 16048 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00085   Thioredoxin

Gene Ontology

Mouse over links to read term descriptions.
GO:0045454   cell redox homeostasis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
21 138 4ekz (A) 355 471 32.00 0.0000034 1 1.30408 -0.75
28 139 2dmm (A) 22 133 35.00 0.00000052 1 1.31853 -0.92
37 139 3h79 (A) 19 125 36.00 0 1 1.3044 -1.84
56 117 1v98 (A) 55 114 40.00 0.0000053 0.86 0.914216 -0.29
91 122 3ehd (A) 79 109 48.00 0.089 0.54 0.69445 0.26

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_136492)

Species Accession Gene Product
Leishmania braziliensis LbrM.06.1030   protein disulfide isomerase
Leishmania donovani LdBPK_061090.1   protein disulfide isomerase
Leishmania infantum LinJ.06.1090   protein disulfide isomerase
Leishmania major LmjF.06.1050   protein disulfide isomerase
Leishmania mexicana LmxM.06.1050   protein disulfide isomerase
Trypanosoma brucei Tb927.7.5790   protein disulfide isomerase, putative
Trypanosoma congolense TcIL3000_7_4770   protein disulfide isomerase, putative
Trypanosoma cruzi TcCLB.508173.150   protein disulfide isomerase, putative
Trichomonas vaginalis TVAG_244540   protein disulfide isomerase, putative
Trichomonas vaginalis TVAG_212700   protein disulfide isomerase, putative
Trichomonas vaginalis TVAG_357940   protein disulfide isomerase, putative
Trichomonas vaginalis TVAG_456860   protein disulfide isomerase, putative
Trichomonas vaginalis TVAG_114230   conserved hypothetical protein
Trichomonas vaginalis TVAG_254820   conserved hypothetical protein
Trichomonas vaginalis TVAG_407230   protein disulfide isomerase, putative
Trichomonas vaginalis TVAG_274680   protein disulfide isomerase, putative
Trichomonas vaginalis TVAG_140820   conserved hypothetical protein
Trichomonas vaginalis TVAG_457030   protein disulfide isomerase, putative
Trichomonas vaginalis TVAG_255840   protein disulfide isomerase, putative
Trichomonas vaginalis TVAG_231810   protein disulfide isomerase, putative

Essentiality

TcCLB.508173.150 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.5790 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.5790 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.5790 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.7.5790 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Protein Disulfide-Isomerase (5.3.4.1 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

  • Tcru011707AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tcru011707; Recombinant protein: full-length; Source: T cruzi; protein disulfide isomerase-related ;

Bibliographic References

5 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.508173.150 (Trypanosoma cruzi), protein disulfide isomerase, putative
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