Detailed view for TcCLB.509099.20

Basic information

TDR Targets ID: 30999
Trypanosoma cruzi, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.1461 | Length (AA): 1050 | MW (Da): 115662 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00782   Dual specificity phosphatase, catalytic domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016791   phosphoric monoester hydrolase activity  
GO:0008138   protein tyrosine/serine/threonine phosphatase activity  
GO:0016311   dephosphorylation  
GO:0006470   protein amino acid dephosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
427 633 2c46 (A) 4 198 12.00 0.0000000000016 0.07 0.11 -0.02
444 603 1vhr (A) 8 180 28.00 0 1 0.5 -1.86
460 602 1zzw (A) 321 464 36.00 0 1 0.71 -2.45
437 601 3f81 (A) 7 178 29.00 0 1 0.587843 -1.33
449 600 2pq5 (A) 32 191 37.00 0 1 0.568762 -0.62
462 600 2nt2 (A) 309 446 30.00 0 1 0.672881 -1.73

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_131582)

Species Accession Gene Product
Arabidopsis thaliana AT3G55270   mitogen-activated protein kinase phosphatase 1
Dictyostelium discoideum DDB_G0282425   hypothetical protein
Dictyostelium discoideum DDB_G0288249   MAP kinase phosphatase
Dictyostelium discoideum DDB_G0271860   hypothetical protein
Leishmania braziliensis LbrM.27.1980   hypothetical protein, conserved
Leishmania donovani LdBPK_271740.1   Dual specificity phosphatase, catalytic domain containing protein, putative
Leishmania infantum LinJ.27.1740   hypothetical protein, conserved
Leishmania major LmjF.27.1840   hypothetical protein, conserved
Leishmania mexicana LmxM.27.1840   hypothetical protein, conserved
Oryza sativa 4337619   Os05g0115800
Plasmodium berghei PBANKA_1223900   protein tyrosine phosphatase
Plasmodium knowlesi PKNH_0112100   protein tyrosine phosphatase, putative
Plasmodium vivax PVX_088210   protein tyrosine phosphatase, putative
Plasmodium yoelii PY05421   Homo sapiens bA386N14.1, putative
Trypanosoma brucei gambiense Tbg.972.2.2380   dual specificity protein phosphatase, putative
Trypanosoma brucei Tb927.2.4280   kinetoplastid-specific dual specificity phosphatase, putative
Trypanosoma congolense TcIL3000_2_730   dual specificity protein phosphatase, putative
Trypanosoma cruzi TcCLB.509099.20   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.509321.10   hypothetical protein, conserved

Essentiality

TcCLB.509099.20 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.2.4280 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.2.4280 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.2.4280 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.2.4280 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1223900 Plasmodium berghei Essential plasmo
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.509099.20 (Trypanosoma cruzi), hypothetical protein, conserved
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