Detailed view for TcCLB.510329.70

Basic information

TDR Targets ID: 32123
Trypanosoma cruzi, cdc2-related kinase 10, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.9933 | Length (AA): 595 | MW (Da): 65241 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0004672   protein kinase activity  
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0006468   protein amino acid phosphorylation  

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 448 1j1b (A) 35 388 26.00 0 1 0.57 0.64
29 397 1z57 (A) 161 478 23.00 0 1 0.77 -0.13
1 332 1rjb (A) 578 937 23.00 0.000000011 1 0.568183 0.24
27 511 3ncg (A) 74 437 24.00 0 1 0.627326 0.82
29 398 4au8 (A) 4 288 43.00 0 1 0.683049 0.23
33 190 2jc6 (A) 27 151 37.00 0.0003 0.9 0.280546 0.91
502 571 4hab (C) 375 456 15.00 0.41 0.07 0.206847 -0.48

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_151756)

Species Accession Gene Product
Leishmania braziliensis LbrM.29.2130   protein kinase, putative
Leishmania donovani LdBPK_292260.1   cdc2-related kinase 10, putative
Leishmania infantum LinJ.29.2260   protein kinase, putative
Leishmania major LmjF.29.2150   protein kinase, putative
Leishmania mexicana LmxM.08_29.2150   protein kinase, putative
Trypanosoma brucei gambiense Tbg972.3.5200   cell division control protein, putative
Trypanosoma brucei Tb927.3.4670   cdc2-related kinase 10, putative
Trypanosoma congolense TcIL3000_3_2870   cdc2-related kinase 10, putative
Trypanosoma cruzi TcCLB.510329.70   cdc2-related kinase 10, putative

Essentiality

TcCLB.510329.70 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.4670 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.3.4670 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.3.4670 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.3.4670 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus c-Jun N-terminal kinase 3 464 aa 21.9% 402 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 25.7% 373 aa Compounds References
Rattus norvegicus Glycogen synthase kinase-3 beta 420 aa 27.0% 381 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 32.1% 234 aa Compounds References
Xenopus laevis Aurora kinase B-A 361 aa 25.3% 296 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 33.4% 365 aa Compounds References
Zea mays Casein kinase II alpha 332 aa 25.5% 290 aa Compounds References
Patiria pectinifera Cdc2 300 aa 29.6% 362 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 32.2% 373 aa Compounds References
Sus scrofa Glycogen synthase kinase 3 beta 420 aa 26.5% 381 aa Compounds References
Schizosaccharomyces pombe 972h- Casein kinase II subunit alpha 332 aa 25.6% 312 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 8 411 aa 22.8% 373 aa Compounds References
Bos taurus Glycogen synthase kinase-3 beta splice variant X1 419 aa 26.8% 381 aa Compounds References
Rattus norvegicus MAP kinase p38 alpha 360 aa 29.1% 371 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 31.4% 369 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0003 0.5 0.5
0.0063 0.7244 0.2543
0.0032 0.5 0.5
0.0029 0.5 0.5
0.0033 0.5 0.5
0.0039 0.5 0.5
0.0059 1 1
0.0032 0.5 0.5
0.0004 0.5 0.5
0.0062 0.6935 0
0.0026 0.5 0.5
0.0033 1 0.5
0.0069 0.3067 1
0.0022 0.5 0.5
0.0027 1 0.5
0.0018 0.5 0.5
0.0061 0.6883 0.5304
0.0008 0.5 0.5
0.0064 0.3377 0
0.0092 1 0.5
0.0039 0.9485 0.5
0.0012 0.5 0.5
0.0088 0.4477 0.5
0.0056 1 0.5
0.0023 0.5 0.5
0.0093 0.8828 0
0.0039 0.5 0.5
0.0063 1 0.5
0.0007 0.5 0.5
0.0042 0.5 0.5
0.0007 0.5 0.5
0.0037 1 0.5
0.0091 1 0.5
0.0059 1 1
0.0011 1 0.5
0.0067 0.5 0.5
0.0012 0.5 0.5
0.0081 1 0.5
0.0066 0.3101 0
0.0081 0.5 0.5
0.0007 0.5 0.5
0.0098 0.3242 0.3649
0.0016 0.5 0.5
0.0012 0.5 0.5
0.0036 0.5 0.5
0.0016 0.5 0.5
0.0059 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

21 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.510329.70 (Trypanosoma cruzi), cdc2-related kinase 10, putative
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