Detailed view for PF3D7_0109400

Basic information

TDR Targets ID: 3214
Plasmodium falciparum, tubulin-specific chaperone a, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 5.2289 | Length (AA): 166 | MW (Da): 19591 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02970   Tubulin binding cofactor A

Gene Ontology

Mouse over links to read term descriptions.
GO:0048487   beta-tubulin binding  
GO:0007023   post-chaperonin tubulin folding pathway  
GO:0005874   microtubule  
GO:0051082   unfolded protein binding  
GO:0007021   tubulin folding  
GO:0006461   protein complex assembly  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
8 98 1h7c (A) 6 95 33.00 0.000069 1 1.04 -1.04
1 140 4uos (A) 9 132 29.00 0.16 0.67 1.10107 -0.27
1 139 4uos (A) 42 184 14.00 0.012 0.46 0.989049 -0.48
5 165 4uos (A) 3 164 16.00 0.0051 0.4 1.25358 -1.21
11 102 1qsd (A) 6 97 23.00 0.63 0.91 0.951917 -1.03

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 24 hs, gametocyte, Oocyst, Sporozoite, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 32 hs, early trophozoite, late trophozoite, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 40 hs, sporozoite, early ring, early schizont. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 48 hs. Otto TD
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_128690)

Species Accession Gene Product
Arabidopsis thaliana AT2G30410   tubulin folding cofactor A protein KIESEL
Brugia malayi Bm1_00990   Tubulin binding cofactor A family protein
Caenorhabditis elegans CELE_Y39G10AR.20   Protein Y39G10AR.20
Dictyostelium discoideum DDB_G0271706   tubulin folding cofactor A
Drosophila melanogaster Dmel_CG1890   CG1890 gene product from transcript CG1890-RA
Giardia lamblia GL50803_1649   Hypothetical protein
Homo sapiens ENSG00000171530   tubulin folding cofactor A
Leishmania braziliensis LbrM.32.3230   tubulin binding cofactor A-like protein
Leishmania donovani LdBPK_323130.1   tubulin binding cofactor A-like protein
Leishmania infantum LinJ.32.3130   tubulin binding cofactor A-like protein
Leishmania major LmjF.32.2970   tubulin binding cofactor A-like protein
Leishmania mexicana LmxM.31.2970   tubulin binding cofactor A-like protein
Loa Loa (eye worm) LOAG_10496   tubulin binding cofactor A family protein
Mus musculus ENSMUSG00000042043   tubulin cofactor A
Neospora caninum NCLIV_002440   tubulin-specific chaperone a, putative
Oryza sativa 4331140   Os02g0816500
Plasmodium berghei PBANKA_0204200   tubulin-specific chaperone a, putative
Plasmodium falciparum PF3D7_0109400   tubulin-specific chaperone a, putative
Plasmodium knowlesi PKNH_0204400   tubulin-specific chaperone a, putative
Plasmodium vivax PVX_081315   tubulin-specific chaperone a, putative
Plasmodium yoelii PY03059   hypothetical protein
Saccharomyces cerevisiae YOR265W   Rbl2p
Schistosoma japonicum Sjp_0207750   IPR004226,Tubulin binding cofactor A,domain-containing
Schistosoma mansoni Smp_019050.1   hypothetical protein
Schistosoma mansoni Smp_019050.2   hypothetical protein
Schmidtea mediterranea mk4.001409.05  
Trypanosoma brucei gambiense Tbg972.11.18120   tubulin binding cofactor A, putative
Trypanosoma brucei Tb927.11.16150   tubulin binding cofactor A, putative
Trypanosoma cruzi TcCLB.509069.30   tubulin binding cofactor A-like protein, putative
Trypanosoma cruzi TcCLB.508707.224   tubulin binding cofactor A-like protein, putative
Toxoplasma gondii TGME49_295310   tubulin binding cofactor A protein
Theileria parva TP04_0623   hypothetical protein
Trichomonas vaginalis TVAG_488940   conserved hypothetical protein
Trichomonas vaginalis TVAG_178070   tubulin-specific chaperone A, putative

Essentiality

PF3D7_0109400 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.7825 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.7825 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.7825 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.7825 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0204200 Plasmodium berghei Dispensable plasmo
TGME49_295310 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_0109400 (Plasmodium falciparum), tubulin-specific chaperone a, putative
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