Detailed view for Tb927.10.450

Basic information

TDR Targets ID: 321786
Trypanosoma brucei, Component of motile flagella 62
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.7633 | Length (AA): 641 | MW (Da): 71879 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF10243   Microtubule-binding protein MIP-T3

Gene Ontology

Mouse over links to read term descriptions.
GO:0008017   microtubule binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_130500)

Species Accession Gene Product
Brugia malayi Bm1_36545   MIP-T3
Caenorhabditis elegans CELE_C02H7.1   Protein DYF-11
Drosophila melanogaster Dmel_CG3259   CG3259 gene product from transcript CG3259-RA
Echinococcus multilocularis EmuJ_000104400   TRAF3 interacting protein 1
Giardia lamblia GL50803_9098   Hypothetical protein
Homo sapiens ENSG00000204104   TNF receptor-associated factor 3 interacting protein 1
Leishmania braziliensis LbrM.21.1760   hypothetical protein, conserved
Leishmania donovani LdBPK_211880.1   Microtubule-binding protein MIP-T3, putative
Leishmania infantum LinJ.21.1880   hypothetical protein, conserved
Leishmania major LmjF.21.1566   hypothetical protein, conserved
Leishmania mexicana LmxM.21.1566   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_04864   hypothetical protein
Mus musculus ENSMUSG00000034292   TRAF3 interacting protein 1
Schistosoma japonicum Sjp_0023800   TRAF3-interacting protein 1, putative
Schistosoma mansoni Smp_173850   hypothetical protein
Schistosoma mansoni Smp_173860   Traf3ip1 protein
Trypanosoma brucei gambiense Tbg972.10.390   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.450   Component of motile flagella 62
Trypanosoma congolense TcIL3000_10_330   Microtubule-binding protein MIP-T3, putative
Trypanosoma cruzi TcCLB.510519.149   Microtubule-binding protein MIP-T3, putative
Trypanosoma cruzi TcCLB.511143.12   Microtubule-binding protein MIP-T3, putative
Trichomonas vaginalis TVAG_109650   caldesmon, putative

Essentiality

Tb927.10.450 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.450 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.450 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.450 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.450 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.450 (Trypanosoma brucei), Component of motile flagella 62
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