Detailed view for PF3D7_0316300

Basic information

TDR Targets ID: 322012
Plasmodium falciparum, inorganic pyrophosphatase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 7.2644 | Length (AA): 431 | MW (Da): 51312 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00719   Inorganic pyrophosphatase

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0004427   inorganic diphosphatase activity  
GO:0000287   magnesium ion binding  
GO:0006796   phosphate metabolic process  

Metabolic Pathways

Oxidative phosphorylation (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
79 368 1e9g (A) 1 284 34.00 0 1 1.12376 -0.21
110 283 4lug (A) 35 180 38.00 0.00000000089 1 0.630595 0.19
189 377 4uos (A) 2 180 17.00 0.57 0.05 0.639668 -0.53
108 366 5c5v (A) 147 410 31.00 0.000000029 1 0.900828 -0.2
130 419 1e9g (A) 1 284 33.00 0 1 1.00615 -0.04
168 351 3sw5 (A) 10 166 35.00 0.00095 1 0.563314 0.25

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 5WRU:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, Oocyst, Sporozoite, Female Gametocyte, Male Gametocyte. Otto TD Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, Ring. Otto TD Zanghi G
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_127487)

Species Accession Gene Product
Arabidopsis thaliana AT5G09650   soluble inorganic pyrophosphatase 1
Babesia bovis BBOV_III010840   inorganic pyrophosphatase family protein
Brugia malayi Bm1_16955   inorganic pyrophosphatase
Candida albicans CaO19.3590   inorganic pyrophosphatase
Candida albicans CaO19.11072   inorganic pyrophosphatase
Caenorhabditis elegans CELE_C47E12.4   Protein PYP-1, isoform D
Cryptosporidium hominis Chro.40159   inorganic pyrophosphatase precursor
Cryptosporidium parvum cgd4_1400   conserved hypothetical protein
Dictyostelium discoideum DDB_G0284265   inorganic pyrophosphatase
Drosophila melanogaster Dmel_CG4634   Nucleosome remodeling factor - 38kD
Entamoeba histolytica EHI_124880   inorganic pyrophosphatase, putative
Homo sapiens ENSG00000138777   pyrophosphatase (inorganic) 2
Homo sapiens ENSG00000180817   pyrophosphatase (inorganic) 1
Leishmania braziliensis LbrM.19.0110   acidocalcisomal pyrophosphatase
Leishmania donovani LdBPK_110210.1   acidocalcisomal pyrophosphatase
Leishmania infantum LinJ.11.0210   acidocalcisomal pyrophosphatase
Leishmania major LmjF.11.0210   acidocalcisomal pyrophosphatase
Leishmania mexicana LmxM.11.0210   acidocalcisomal pyrophosphatase
Loa Loa (eye worm) LOAG_03390   inorganic pyrophosphatase
Mus musculus ENSMUSG00000028013   pyrophosphatase (inorganic) 2
Mus musculus ENSMUSG00000020089   pyrophosphatase (inorganic) 1
Neospora caninum NCLIV_028850   soluble inorganic pyrophosphatase, putative
Oryza sativa 4330852   Os02g0768600
Plasmodium berghei PBANKA_0414100   inorganic pyrophosphatase, putative
Plasmodium falciparum PF3D7_0316300   inorganic pyrophosphatase, putative
Plasmodium knowlesi PKNH_0825800   inorganic pyrophosphatase, putative
Plasmodium vivax PVX_095420   inorganic pyrophosphatase, putative
Plasmodium yoelii PY03581   inorganic pyrophosphatase, putative
Saccharomyces cerevisiae YBR011C   inorganic diphosphatase IPP1
Schmidtea mediterranea mk4.000077.06  
Trypanosoma brucei gambiense Tbg972.11.8000   acidocalcisomal pyrophosphatase, putative
Trypanosoma brucei gambiense Tbg972.11.8020   acidocalcisomal pyrophosphatase, putative
Trypanosoma brucei Tb927.11.7060   acidocalcisomal pyrophosphatase
Trypanosoma brucei Tb927.11.7080   acidocalcisomal pyrophosphatase
Trypanosoma congolense TcIL3000_0_38820   acidocalcisomal pyrophosphatase
Trypanosoma congolense TcIL3000.11.7660   acidocalcisomal pyrophosphatase
Trypanosoma cruzi TcCLB.511165.40   acidocalcisomal pyrophosphatase, putative
Trypanosoma cruzi TcCLB.503613.60   acidocalcisomal pyrophosphatase, putative
Toxoplasma gondii TGME49_283830   type I inorganic pyrophosphatase PPase
Theileria parva TP02_0061   inorganic pyrophosphatase, putative

Essentiality

PF3D7_0316300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.4930 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.02.4930 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.4930 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.4930 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.02.4910 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.4910 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.4910 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.4910 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C47E12.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_C47E12.4 Caenorhabditis elegans larval arrest wormbase
CELE_C47E12.4 Caenorhabditis elegans larval lethal wormbase
CELE_C47E12.4 Caenorhabditis elegans slow growth wormbase
CELE_C47E12.4 Caenorhabditis elegans sterile wormbase
YBR011C Saccharomyces cerevisiae inviable yeastgenome
TGME49_283830 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PF3D7_0316300 (Plasmodium falciparum), inorganic pyrophosphatase, putative
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