Detailed view for TGME49_300060

Basic information

TDR Targets ID: 323580
Toxoplasma gondii, signal peptidase subunit protein
Source Database / ID:  ToxoDB 

pI: 6.2218 | Length (AA): 175 | MW (Da): 19500 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04573   Signal peptidase subunit

Gene Ontology

Mouse over links to read term descriptions.
GO:0005787   signal peptidase complex  
GO:0016021   integral to membrane  
GO:0008233   peptidase activity  
GO:0006465   signal peptide processing  

Metabolic Pathways

Lysine degradation (KEGG)
Global map (KEGG)
Protein export (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
53 126 3myv (A) 133 219 38.00 0.71 0.14 0.498557 0.62
90 169 2plw (A) 42 139 30.00 0.41 0.05 0.551843 0.24

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_128397)

Species Accession Gene Product
Arabidopsis thaliana AT3G05230   Signal peptidase complex subunit 3A
Arabidopsis thaliana AT5G27430   Signal peptidase complex subunit 3B
Babesia bovis BBOV_III002870   signal peptidase family protein
Brugia malayi Bm1_16935   Signal peptidase subunit family protein
Candida albicans CaO19.12396   signal peptidase subunit
Candida albicans CaO19.4930   signal peptidase subunit
Caenorhabditis elegans CELE_K12H4.4   Protein K12H4.4
Cryptosporidium hominis Chro.30306   hypothetical protein
Cryptosporidium parvum cgd3_2680   possible signal peptidase subunit, signal peptide
Dictyostelium discoideum DDB_G0290851   microsomal signal peptidase subunit
Drosophila melanogaster Dmel_CG5677   Spase 22/23-subunit
Echinococcus granulosus EgrG_000866100   signal peptidase complex subunit 3
Entamoeba histolytica EHI_121860   microsomal signal peptidase subunit, putative
Echinococcus multilocularis EmuJ_000866100   signal peptidase complex subunit 3
Giardia lamblia GL50803_2964   hypothetical protein
Homo sapiens ENSG00000129128   signal peptidase complex subunit 3 homolog (S. cerevisiae)
Loa Loa (eye worm) LOAG_03394   signal peptidase subunit family protein
Mus musculus ENSMUSG00000048040   adipocyte-related X-chromosome expressed sequence 2
Mus musculus ENSMUSG00000054408   signal peptidase complex subunit 3 homolog (S. cerevisiae)
Mus musculus ENSMUSG00000048355   adipocyte-related X-chromosome expressed sequence 1
Neospora caninum NCLIV_064640   Probable signal peptidase complex subunit 3 (EC 3.4.-.-), related
Oryza sativa 4325792   Os01g0131800
Oryza sativa 4347817   Os09g0556000
Onchocerca volvulus OVOC9016   Putative signal peptidase complex subunit 3
Plasmodium berghei PBANKA_0417000   signal peptidase complex subunit 3, putative
Plasmodium falciparum PF3D7_0904400   signal peptidase complex subunit 3, putative
Plasmodium knowlesi PKNH_0702100   signal peptidase complex subunit 3, putative
Plasmodium vivax PVX_098665   signal peptidase complex subunit 3, putative
Plasmodium yoelii PY04820   probable microsomal signal peptidase 22 kDa subunit, putative
Saccharomyces cerevisiae YLR066W   Spc3p
Schistosoma japonicum Sjp_0200670   ko:K01423 Spase22-23; Spase 22/23-subunit [EC:3.4.-.-], putative
Schistosoma mansoni Smp_175420   microsomal signal peptidase 23 kD subunit (spc22/23)
Schmidtea mediterranea mk4.001249.02   Probable signal peptidase complex subunit 3
Toxoplasma gondii TGME49_300060   signal peptidase subunit protein
Theileria parva TP04_0108   signal peptidase, putative
Trichomonas vaginalis TVAG_455170   Signal peptidase complex subunit, putative

Essentiality

TGME49_300060 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_K12H4.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_K12H4.4 Caenorhabditis elegans larval lethal wormbase
CELE_K12H4.4 Caenorhabditis elegans slow growth wormbase
CELE_K12H4.4 Caenorhabditis elegans sterile wormbase
YLR066W Saccharomyces cerevisiae inviable yeastgenome
TGME49_300060 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_300060 (Toxoplasma gondii), signal peptidase subunit protein
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