Detailed view for TGME49_263360

Basic information

TDR Targets ID: 324326
Toxoplasma gondii, WD domain, G-beta repeat-containing protein
Source Database / ID:  ToxoDB 

pI: 9.2632 | Length (AA): 1581 | MW (Da): 170047 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
467 575 2mqa (A) 33 140 12.00 0 0.01 0.174544 -0.25
927 1198 2h9l (A) 14 288 16.00 0 0.62 0.276943 0.53
947 1197 5kdo (B) 43 340 25.00 0.61 1 0.19216 0.14
955 1033 4j8b (A) 133 241 38.00 0.0067 0.7 0.353368 0.34
955 1103 2hes (X) 149 277 32.00 0.009 0.35 0.233644 0.23
956 1235 3sfz (A) 837 1106 26.00 0.15 1 0.195503 0.99
976 1033 4gqb (B) 271 327 19.00 0 0.13 0.175586 0.15
1078 1205 4aez (A) 224 337 31.00 0.59 0.12 0.167361 0.73
1078 1198 4wjv (A) 189 306 27.00 0.54 0.97 0.274934 -0.24
1377 1472 3k5m (A) 363 454 36.00 0.057 0.06 0.245121 0.99

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite, ME49 Oocyst. Hehl AB Fritz HM
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_129210)

Species Accession Gene Product
Arabidopsis thaliana AT2G37670   WD-40 repeat-containing protein
Arabidopsis thaliana AT5G02430   transducin/WD40 domain-containing protein
Arabidopsis thaliana AT3G15470   WD40 domain-containing protein
Arabidopsis thaliana AT5G54200   transducin/WD40 domain-containing protein
Brugia malayi Bm1_14955   Hypothetical WD-repeats containing protein YKL121w, putative
Candida albicans CaO19.7235   similar to S. pombe SPAC3H5.08c and to S. cerevisiae YKL121W
Caenorhabditis elegans CELE_R03E1.1   Protein SYM-4
Cryptosporidium hominis Chro.70601   RIKEN cDNA 2610034K17
Cryptosporidium parvum cgd7_5410   WD40 protein
Dictyostelium discoideum DDB_G0282491   hypothetical protein
Drosophila melanogaster Dmel_CG34133   CG34133 gene product from transcript CG34133-RC
Echinococcus granulosus EgrG_000761600   WD repeat protein
Echinococcus multilocularis EmuJ_000761600   WD repeat protein
Homo sapiens ENSG00000131725   WD repeat domain 44
Mus musculus ENSMUSG00000036769   WD repeat domain 44
Oryza sativa 9267200   Os12g0178633
Oryza sativa 4337413   Os04g0678300
Oryza sativa 9269146   Os04g0529400
Oryza sativa 9270524   Os11g0187000
Saccharomyces cerevisiae YMR102C   hypothetical protein
Schistosoma japonicum Sjp_0216010   expressed protein
Schistosoma mansoni Smp_171200   hypothetical protein
Schmidtea mediterranea mk4.001347.03   Putative wd-repeat protein
Toxoplasma gondii TGME49_263360   WD domain, G-beta repeat-containing protein
Trichomonas vaginalis TVAG_078530   WD repeat domain, putative
Trichomonas vaginalis TVAG_135970   WD repeat domain, putative

Essentiality

TGME49_263360 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_R03E1.1 Caenorhabditis elegans slow growth wormbase
TGME49_263360 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_263360 (Toxoplasma gondii), WD domain, G-beta repeat-containing protein
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