Detailed view for TcCLB.508409.160

Basic information

TDR Targets ID: 32798
Trypanosoma cruzi, thymine-7-hydroxylase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.9588 | Length (AA): 319 | MW (Da): 36695 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03171   2OG-Fe(II) oxygenase superfamily
PF14226   non-haem dioxygenase in morphine synthesis N-terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0016491   oxidoreductase activity  
GO:0005506   iron ion binding  
GO:0055114   oxidation reduction  

Metabolic Pathways

Glyoxylate and dicarboxylate metabolism (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 319 1gp6 (A) 43 350 26.00 0 1 1.35 -1.29
7 305 1odm (A) 10 318 33.00 0 1 1.36 -1.22
1 312 1odm (A) 4 325 27.00 0 1 1.38236 -1.04
6 280 1w9y (A) 4 259 36.00 0 1 1.23387 -0.59
6 274 5c3o (A) 13 295 35.00 0.000036 1 1.27776 -1.22
6 312 5c3q (A) 13 333 33.00 0 1 1.44188 -1.17

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126909)

Species Accession Gene Product
Arabidopsis thaliana AT3G49620   2-oxoacid-dependent dioxygenase-like protein DIN11
Arabidopsis thaliana AT1G47990   gibberellin 2-oxidase 4
Arabidopsis thaliana AT3G49630   2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Arabidopsis thaliana AT1G78440   gibberellin 2-beta-dioxygenase 1
Arabidopsis thaliana AT3G50210   2-oxoglutarate-Fe(II)-dependent oxygenase domain-containing protein
Arabidopsis thaliana AT3G46490   oxidoreductase, 2OG-Fe(II) oxygenase family protein
Arabidopsis thaliana AT1G35190   2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Arabidopsis thaliana AT4G16770   iron ion binding / oxidoreductase
Arabidopsis thaliana AT4G16765   oxidoreductase, 2OG-Fe(II) oxygenase family protein
Candida albicans CaO19.541   Fe II, 2-oxoglutarate-dependent dioxygenases
Candida albicans CaO19.8174   Fe II, 2-oxoglutarate-dependent dioxygenases
Candida albicans CaO19.1306   conserved hypothetical protein, similar to Fe II, 2-oxoglutarate-dependent dioxygenases
Candida albicans CaO19.8886   conserved hypothetical protein, similar to Fe II, 2-oxoglutarate-dependent dioxygenases
Dictyostelium discoideum DDB_G0286201   hypothetical protein
Dictyostelium discoideum DDB_G0277497   hypothetical protein
Dictyostelium discoideum DDB_G0286203   hypothetical protein
Dictyostelium discoideum DDB_G0283291   hypothetical protein
Drosophila melanogaster Dmel_CG33099   CG33099 gene product from transcript CG33099-RA
Drosophila melanogaster Dmel_CG33093   CG33093 gene product from transcript CG33093-RA
Drosophila melanogaster Dmel_CG5346   CG5346 gene product from transcript CG5346-RA
Leishmania braziliensis LbrM.29.0510   thymine-7-hydroxylase, putative
Leishmania donovani LdBPK_290260.1   thymine-7-hydroxylase, putative
Leishmania infantum LinJ.29.0260   thymine-7-hydroxylase, putative;with=GeneDB:Tb927.7.7500
Leishmania major LmjF.29.0250   thymine-7-hydroxylase, putative;with=GeneDB:Tb927.7.7500
Leishmania mexicana LmxM.08_29.0250   oxidase-like protein
Leishmania mexicana LmxM.08_29.0251  
Oryza sativa 4326767   Os01g0188100
Oryza sativa 4346490   Os09g0245500
Oryza sativa 4349154   Os10g0522900
Oryza sativa 4349156   Os10g0523100
Oryza sativa 4346502   Os09g0248900
Trypanosoma brucei gambiense Tbg972.7.8820   iron/ascorbate oxidoreductase family protein
Trypanosoma brucei gambiense Tbg972.5.190   iron/ascorbate oxidoreductase family protein, putative
Trypanosoma brucei Tb927.9.14600   iron/ascorbate oxidoreductase family protein, putative
Trypanosoma brucei Tb927.7.7500   thymine-7-hydroxylase, putative
Trypanosoma brucei Tb11.v5.0215   iron/ascorbate oxidoreductase family protein, putative
Trypanosoma brucei Tb927.5.300   thymine-7-hydroxylase, putative
Trypanosoma congolense TcIL3000_0_46870   2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein, putative
Trypanosoma congolense TcIL3000_0_09620   2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein, putative
Trypanosoma cruzi TcCLB.508409.160   thymine-7-hydroxylase, putative
Trypanosoma cruzi TcCLB.510141.10   thymine-7-hydroxylase, putative

Essentiality

TcCLB.508409.160 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.7500 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.7500 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.7500 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.7500 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.5.300 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.300 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.300 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.300 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb09.211.4990 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.4990 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.4990 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.4990 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Oxalate Oxidase (1.2.3.4 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

  • Tcru012735AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tcru012735; Recombinant protein: full-length; Source: T cruzi; probable oxidoreductase PA0147 (imported) - Pseudomonas aeruginosa (strain PAO1) ;

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.508409.160 (Trypanosoma cruzi), thymine-7-hydroxylase, putative
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