Detailed view for TcCLB.507047.150

Basic information

TDR Targets ID: 33417
Trypanosoma cruzi, L-galactonolactone oxidase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.7037 | Length (AA): 505 | MW (Da): 56829 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01565   FAD binding domain
PF04030   D-arabinono-1,4-lactone oxidase

Gene Ontology

Mouse over links to read term descriptions.
GO:0003885   D-arabinono-1,4-lactone oxidase activity  
GO:0016899   oxidoreductase activity, acting on the CH-OH group of donors, oxygen as acceptor  
GO:0016614   oxidoreductase activity, acting on CH-OH group of donors  
GO:0016020   membrane  
GO:0003824   catalytic activity  
GO:0050660   FAD binding  
GO:0016491   oxidoreductase activity  
GO:0055114   oxidation reduction  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 495 1wvf (A) 34 520 9.00 0 1 1.01 -0.39
1 484 1w1o (A) 47 527 14.00 0 1 1.14 -0.54
6 491 3js8 (A) 14 538 17.00 0 1 0.915776 0.45
9 491 2vfr (A) 4 415 24.00 0 1 1.07484 0.3
32 189 4aut (A) 41 203 29.00 0.0000035 1 0.710971 -0.43
67 183 5ae2 (A) 255 380 31.00 0.11 1 0.593783 -0.27

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_129861)

Species Accession Gene Product
Arabidopsis thaliana AT3G47930   L-galactono-1,4-lactone dehydrogenase
Candida albicans CaO19.7551   D-arabinono-1,4-lactone oxidase
Leishmania braziliensis LbrM.17.1490   L-gulonolactone oxidase, putative
Leishmania donovani LdBPK_171470.1   L-galactonolactone oxidase, putative
Leishmania infantum LinJ.17.1470   L-gulonolactone oxidase, putative
Leishmania major LmjF.17.1360   L-gulonolactone oxidase, putative
Leishmania mexicana LmxM.17.1360   L-gulonolactone oxidase, putative
Mus musculus ENSMUSG00000034450   gulonolactone (L-) oxidase
Mycobacterium tuberculosis Rv1771   L-gulono-1,4-lactone dehydrogenase
Mycobacterium ulcerans MUL_3124   oxidoreductase
Oryza sativa 4349749   Os11g0143500
Oryza sativa 4351464   Os12g0139600
Saccharomyces cerevisiae YML086C   D-arabinono-1,4-lactone oxidase
Schmidtea mediterranea mk4.058992.00  
Trypanosoma brucei gambiense Tbg972.5.3730   hypothetical protein, conserved
Trypanosoma brucei Tb927.5.2650   L-galactonolactone oxidase
Trypanosoma congolense TcIL3000_5_2700   L-galactonolactone oxidase 2, putative
Trypanosoma congolense TcIL3000_5_2600   L-galactonolactone oxidase 1, putative
Trypanosoma cruzi TcCLB.507047.150   L-galactonolactone oxidase, putative
Trypanosoma cruzi TcCLB.509179.100   L-galactonolactone oxidase

Essentiality

TcCLB.507047.150 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.2650 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.2650 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.2650 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.2650 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
catalytic activity (GO:0003824) decreased (PATO:0000468) inferred from loss-of-function mutant phenotype (ECO:0000016) Trypanosoma cruzi No drug identifiers listed for this gene.
Annotator: millerja@u.washington.edu. Comment: Enzyme involved in final step in vitamin C synthesis; lacking in humans. References: 17627608

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier TcCLB.507047.150 (Trypanosoma cruzi), L-galactonolactone oxidase, putative
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