Detailed view for TcCLB.503419.30

Basic information

TDR Targets ID: 33557
Trypanosoma cruzi, thiol-dependent reductase 1, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.6717 | Length (AA): 445 | MW (Da): 50682 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13417   Glutathione S-transferase, N-terminal domain
PF16865   Glutathione S-transferase, C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0015035   protein disulfide oxidoreductase activity  
GO:0009055   electron carrier activity  
GO:0005515   protein binding  
GO:0045454   cell redox homeostasis  

Metabolic Pathways

Chagas disease (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 227 1eem (A) 22 234 16.00 0 1 0.73 -1.32
4 193 1ljr (A) 3 199 25.00 0 1 0.83 -1.61
216 442 1eem (A) 12 231 24.00 0 1 0.88 -1.42
2 439 4ags (A) 4 442 46.00 0 1 1.62557 -1.16
4 208 5g5a (A) 5 206 22.00 0 1 0.863874 -1.26
239 320 2v6k (A) 12 93 37.00 0.00043 0.81 0.58617 -0.3

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_126817)

Species Accession Gene Product
Arabidopsis thaliana AT1G10370   glutathione S-transferase U17
Arabidopsis thaliana AT1G17190   glutathione S-transferase tau 26
Arabidopsis thaliana AT3G55040   glutathione transferase lambda 2
Arabidopsis thaliana AT1G17180   glutathione S-transferase TAU 25
Arabidopsis thaliana AT1G59670   glutathione S-transferase TAU 15
Arabidopsis thaliana AT3G43800   glutathione S-transferase tau 27
Arabidopsis thaliana AT1G78360   glutathione S-transferase TAU 21
Arabidopsis thaliana AT1G78320   glutathione S-transferase TAU 23
Arabidopsis thaliana AT1G59700   glutathione S-transferase TAU 16
Arabidopsis thaliana AT1G78340   glutathione S-transferase TAU 22
Arabidopsis thaliana AT1G10360   glutathione S-transferase TAU 18
Arabidopsis thaliana AT1G17170   glutathione S-transferase TAU 24
Arabidopsis thaliana AT1G78370   glutathione S-transferase TAU 20
Arabidopsis thaliana AT1G78380   glutathione S-transferase TAU 19
Brugia malayi Bm1_44840   Glutathione S-transferase, N-terminal domain containing protein
Brugia malayi Bm1_44425   Glutathione S-transferase, N-terminal domain containing protein
Caenorhabditis elegans CELE_C02D5.3   Protein GSTO-2
Caenorhabditis elegans CELE_K10F12.4   Protein GSTO-3, isoform B
Caenorhabditis elegans CELE_C02D5.4   Protein C02D5.4
Caenorhabditis elegans CELE_C29E4.7   Protein GSTO-1
Caenorhabditis elegans CELE_F13A7.10   Protein GST-44
Drosophila melanogaster Dmel_CG6776   Glutathione S transferase O3
Drosophila melanogaster Dmel_CG6662   Glutathione S transferase O1
Drosophila melanogaster Dmel_CG6781   sepia
Drosophila melanogaster Dmel_CG6673   Glutathione S transferase O2
Escherichia coli b3592   glutathione S-transferase homolog
Escherichia coli b3229   stringent starvation protein A, phage P1 late gene activator, RNAP-associated acid-resistance protein, inactive glutathione S-tr
Echinococcus granulosus EgrG_000919600   stringent starvation protein A
Echinococcus multilocularis EmuJ_000919600   stringent starvation protein A
Homo sapiens ENSG00000148834   glutathione S-transferase omega 1
Leishmania braziliensis LbrM.31.0550   thiol-dependent reductase 1
Leishmania donovani LdBPK_330260.1   thiol-dependent reductase 1
Leishmania infantum LinJ.33.0260   thiol-dependent reductase 1
Leishmania major LmjF.33.0240   thiol-dependent reductase 1
Leishmania mexicana LmxM.32.0240   thiol-dependent reductase 1
Loa Loa (eye worm) LOAG_10567   hypothetical protein
Loa Loa (eye worm) LOAG_11393   glutathione S-transferase domain-containing protein
Mus musculus ENSMUSG00000025068   glutathione S-transferase omega 1
Oryza sativa 4349190   Os10g0528400
Oryza sativa 4349207   Os10g0530900
Oryza sativa 9268428   Os10g0365300
Oryza sativa 4349197   Os10g0529800
Oryza sativa 4349205   Os10g0530700
Oryza sativa 4349202   Os10g0530400
Oryza sativa 4332456   Os03g0283200
Oryza sativa 4343183   Os07g0468100
Oryza sativa 4349201   Os10g0530300
Oryza sativa 4332455   Os03g0283100
Oryza sativa 4324574   Os01g0558100
Oryza sativa 4349181   Os10g0527400
Oryza sativa 4349192   Os10g0529300
Oryza sativa 4325643   Os01g0692100
Oryza sativa 4349189   Os10g0528300
Oryza sativa 4349199   Os10g0530000
Oryza sativa 4349204   Os10g0530600
Oryza sativa 4349203   Os10g0530500
Oryza sativa 4349200   Os10g0530200
Oryza sativa 4349194   Os10g0529500
Oryza sativa 4346877   Os09g0367700
Oryza sativa 4349188   Os10g0528200
Oryza sativa 9266442   Os10g0527601
Oryza sativa 4349198   Os10g0529900
Oryza sativa 4349169   Os10g0525600
Oryza sativa 4349187   Os10g0528100
Oryza sativa 4349210   Os10g0531400
Oryza sativa 4349185   Os10g0527800
Oryza sativa 4349191   Os10g0528900
Oryza sativa 4349196   Os10g0529700
Oryza sativa 4334347   Os03g0785900
Onchocerca volvulus OVOC8533  
Onchocerca volvulus OVOC8535  
Onchocerca volvulus OVOC12236  
Onchocerca volvulus OVOC8665  
Onchocerca volvulus OVOC12238  
Onchocerca volvulus OVOC12237  
Schistosoma japonicum Sjp_0028180   ko:K03599 stringent starvation protein A, putative
Schistosoma mansoni Smp_152710.1   glutathione-s-transferase omega
Schistosoma mansoni Smp_152710.2   glutathione-s-transferase omega
Schmidtea mediterranea mk4.000154.22   Glutathione-s-transferase omega,putative
Trypanosoma cruzi TcCLB.503419.30   thiol-dependent reductase 1, putative
Trypanosoma cruzi TcCLB.509105.70   thiol-dependent reductase 1, putative

Essentiality

TcCLB.503419.30 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b3229 Escherichia coli non-essential goodall
b3592 Escherichia coli non-essential goodall
CELE_C29E4.7 Caenorhabditis elegans embryonic lethal wormbase
CELE_C29E4.7 Caenorhabditis elegans slow growth wormbase
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens glutathione S-transferase omega 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.503419.30 (Trypanosoma cruzi), thiol-dependent reductase 1, putative
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