Detailed view for TcCLB.506247.460

Basic information

TDR Targets ID: 33814
Trypanosoma cruzi, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.898 | Length (AA): 379 | MW (Da): 42037 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01399   PCI domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008180   signalosome  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 372 4d10 (D) 16 395 23.00 0 1 1.18299 0.3
12 132 5hrz (A) 4 114 14.00 0.0014 0.83 0.430761 0.02
115 194 1pgv (A) 297 379 33.00 0.95 0.31 0.504182 0
241 343 3t5x (A) 274 398 10.00 0 0.22 0.199868 -0.43

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_129642)

Species Accession Gene Product
Arabidopsis thaliana AT5G42970   COP9 signalosome complex subunit 4
Brugia malayi Bm1_40815   PCI domain containing protein
Caenorhabditis elegans CELE_Y55F3AM.15   Protein CSN-4
Dictyostelium discoideum DDB_G0293844   COP9 signalosome complex subunit 4
Drosophila melanogaster Dmel_CG8725   COP9 complex homolog subunit 4
Echinococcus granulosus EgrG_000688950   cop9 complex subunit
Echinococcus multilocularis EmuJ_000688950   cop9 complex subunit
Homo sapiens ENSG00000138663   COP9 signalosome subunit 4
Loa Loa (eye worm) LOAG_04090   PCI domain-containing protein
Mus musculus ENSMUSG00000035297   COP9 (constitutive photomorphogenic) homolog, subunit 4 (Arabidopsis thaliana)
Oryza sativa 4331948   Os03g0197400
Schistosoma japonicum Sjp_0046570   COP9 signalosome complex subunit 4, putative
Schistosoma mansoni Smp_085640.1   cop9 complex subunit
Schistosoma mansoni Smp_085640.2   cop9 complex subunit
Schmidtea mediterranea mk4.000528.11   COP9 signalosome complex subunit 4
Trypanosoma brucei gambiense Tbg972.10.9560   cop9 signalosome complex subunit, putative,predicted PCI domain protein
Trypanosoma brucei Tb927.10.7800   cop9 signalosome complex subunit, putative
Trypanosoma congolense TcIL3000_10_6660   predicted PCI domain protein
Trypanosoma cruzi TcCLB.503779.100   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506247.460   hypothetical protein, conserved
Trichomonas vaginalis TVAG_043930   COP9 signalosome complex subunit, putative
Trichomonas vaginalis TVAG_492560   COP9 signalosome complex subunit, putative

Essentiality

TcCLB.506247.460 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.7800 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.7800 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.7800 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.7800 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y55F3AM.15 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y55F3AM.15 Caenorhabditis elegans larval arrest wormbase
CELE_Y55F3AM.15 Caenorhabditis elegans sterile wormbase
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.506247.460 (Trypanosoma cruzi), hypothetical protein, conserved
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