Detailed view for TcCLB.508777.90

Basic information

TDR Targets ID: 34077
Trypanosoma cruzi, ATP-dependent DNA helicase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.9029 | Length (AA): 834 | MW (Da): 92306 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00580   UvrD/REP helicase N-terminal domain
PF13361   UvrD-like helicase C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016787   hydrolase activity  
GO:0005524   ATP binding  
GO:0004003   ATP-dependent DNA helicase activity  
GO:0003677   DNA binding  
GO:0006281   DNA repair  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
27 822 1pjr () 6 651 22.00 0 1 0.56 0.76
722 807 1qhh (D) 557 647 35.00 0 0.45 0.43 -0.19
10 123 1wx1 (A) 120 239 34.00 0.32 0.07 0.307191 0.89
27 784 3pjr (A) 6 625 24.00 0 1 0.835473 0.96
261 359 1w36 (B) 370 468 30.00 0.00061 0.94 0.454205 0.11
642 780 3dmn (A) 609 747 22.00 0 0.88 0.391267 -0.19
736 807 1qhh (D) 571 647 31.00 0.00021 0.17 0.255831 0.46

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127542)

Species Accession Gene Product
Arabidopsis thaliana AT4G25120   helicase SRS2-like protein
Candida albicans CaO19.13391   similar to S. cerevisiae HPR5 (YJL092W) DNA helicase involved in double strand break repair
Candida albicans CaO19.5970   similar to S. cerevisiae HPR5 (YJL092W) DNA helicase involved in double strand break repair
Cryptosporidium hominis Chro.80373   helicase-like protein nhl
Cryptosporidium parvum cgd8_3210   UvrD like super family I helicase involved in nucleotide excision repair, possible bacterial horizontal transfer
Chlamydia trachomatis CT_608   DNA helicase
Escherichia coli b3813   DNA-dependent ATPase I and helicase II
Escherichia coli b3778   DNA helicase and single-stranded DNA-dependent ATPase
Leishmania braziliensis LbrM.09.0670   ATP-dependent DNA helicase, putative
Leishmania donovani LdBPK_090640.1   ATP-dependent DNA helicase, putative
Leishmania infantum LinJ.09.0640   ATP-dependent DNA helicase, putative
Leishmania major LmjF.09.0590   ATP-dependent DNA helicase, putative
Leishmania mexicana LmxM.09.0590   ATP-dependent DNA helicase, putative
Mycobacterium leprae ML0153   Probable ATP dependent DNA helicase UvrD1
Mycobacterium tuberculosis Rv0949   Probable ATP-dependent DNA helicase II UvrD1
Mycobacterium ulcerans MUL_4723   ATP-dependent DNA helicase II UvrD1
Oryza sativa 4343276   Os07g0492100
Plasmodium berghei PBANKA_1113800   ATP-dependent DNA helicase UvrD, putative
Plasmodium falciparum PF3D7_0514100   ATP-dependent DNA helicase UvrD
Plasmodium knowlesi PKNH_1019000   ATP-dependent DNA helicase UvrD, putative
Plasmodium vivax PVX_080535   ATP-dependent DNA helicase UvrD, putative
Plasmodium yoelii PY02817   probable DNA helicase ii homolog
Plasmodium yoelii PY01998   hypothetical protein
Saccharomyces cerevisiae YJL092W   Srs2p
Schmidtea mediterranea mk4.043254.00  
Trypanosoma brucei gambiense Tbg972.11.14060   ATP-dependent DNA helicase, putative
Trypanosoma brucei Tb927.11.12600   ATP-dependent DNA helicase, putative
Trypanosoma congolense TcIL3000_0_58020   ATP-dependent DNA helicase, putative
Trypanosoma cruzi TcCLB.508777.90   ATP-dependent DNA helicase, putative
Trypanosoma cruzi TcCLB.509029.120   ATP-dependent DNA helicase, putative
Treponema pallidum TP0102   rep helicase, single-stranded DNA-dependent ATPase (rep)
Treponema pallidum TP1028   DNA helicase II (uvrD)
Trichomonas vaginalis TVAG_296350   ATP-dependent DNA helicase rep, putative
Wolbachia endosymbiont of Brugia malayi Wbm0628   superfamily I DNA/RNA helicase

Essentiality

TcCLB.508777.90 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu964 Mycobacterium tuberculosis essential nmpdr
Tb11.01.4440 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.4440 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.4440 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.4440 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b3778 Escherichia coli non-essential goodall
b3813 Escherichia coli non-essential goodall
PBANKA_1113800 Plasmodium berghei Dispensable plasmo
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.004 0.5 0.5
0.004 0.5 0.5
0.0073 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.508777.90 (Trypanosoma cruzi), ATP-dependent DNA helicase, putative
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