Detailed view for TcCLB.503425.29

Basic information

TDR Targets ID: 34401
Trypanosoma cruzi, DNA replication licensing factor MCM8, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.177 | Length (AA): 446 | MW (Da): 48276 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00493   MCM2/3/5 family
PF17207   MCM OB domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0003677   DNA binding  
GO:0006270   DNA replication initiation  
GO:0006260   DNA replication  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
27 282 1ltl (A) 4 238 22.00 0 1 0.63 0.11
314 432 1g41 (A) 2 262 24.00 0.00000095 0.35 0.27 -0.05

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_129017)

Species Accession Gene Product
Arabidopsis thaliana AT3G09660   minichromosome maintenance 8
Dictyostelium discoideum DDB_G0283009   MCM family protein
Drosophila melanogaster Dmel_CG31293   recombination-defective
Echinococcus granulosus EgrG_000772700   dna replication licensing factor mcm8
Entamoeba histolytica EHI_140670   DNA replication licensing factor, putative
Echinococcus multilocularis EmuJ_000772700   dna replication licensing factor mcm8
Homo sapiens ENSG00000125885   minichromosome maintenance complex component 8
Leishmania braziliensis LbrM.05.0320   DNA replication licensing factor, putative,minichromosome maintenance protein-like protein
Leishmania donovani LdBPK_050330.1   DNA replication licensing factor, putative
Leishmania infantum LinJ.05.0330   DNA replication licensing factor, putative,minichromosome maintenance protein-like protein
Leishmania major LmjF.05.0330   DNA replication licensing factor, putative,minichromosome maintenance protein-like protein
Leishmania mexicana LmxM.05.0330   DNA replication licensing factor, putative,minichromosome maintenance protein-like protein
Mus musculus ENSMUSG00000027353   minichromosome maintenance deficient 8 (S. cerevisiae)
Neospora caninum NCLIV_058290   DNA replication licensing factor, putative
Oryza sativa 4339038   Os05g0464100
Plasmodium berghei PBANKA_0609800   DNA helicase MCM8, putative
Plasmodium falciparum PF3D7_1211300   DNA helicase MCM8, putative
Plasmodium knowlesi PKNH_1311400   DNA helicase MCM8, putative
Plasmodium vivax PVX_084595   DNA replication licensing factor MCM8, putative
Plasmodium yoelii PY02431   MCM2/3/5 family
Schistosoma japonicum Sjp_0046030   Conserved hypothetical protein
Schistosoma japonicum Sjp_0046020   expressed protein
Schistosoma japonicum Sjp_0098620   DNA replication licensing factor MCM8, putative
Schistosoma japonicum Sjp_0119450   hypothetical protein
Schistosoma japonicum Sjp_0079950   ko:K10737 minichromosome maintenance protein 8, putative
Schistosoma japonicum Sjp_0099600   hypothetical protein
Schistosoma mansoni Smp_169450   DNA replication licensing factor MCM8
Schmidtea mediterranea mk4.000367.10   DNA replication licensing factor MCM8, putative
Schmidtea mediterranea mk4.000367.11   DNA replication licensing factor REC
Trypanosoma brucei gambiense Tbg972.10.12700   minichromosome maintenance (MCM) complex subunit, putative,minichromosome maintenance protein-like protein
Trypanosoma brucei Tb927.10.10410   DNA replication licensing factor MCM8, putative
Trypanosoma brucei Tb11.v5.0535   minichromosome maintenance (MCM) complex subunit, putative
Trypanosoma congolense TcIL3000_10_8890   DNA replication licensing factor MCM8, putative
Trypanosoma cruzi TcCLB.503425.29   DNA replication licensing factor MCM8, putative
Trypanosoma cruzi TcCLB.503555.10   DNA replication licensing factor MCM8, putative
Toxoplasma gondii TGME49_315600   MCM2/3/5 family protein
Theileria parva TP01_0666   DNA replication licensing factor MCM4, putative

Essentiality

TcCLB.503425.29 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.10410 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.10410 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.10410 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.10410 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_315600 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.503425.29 (Trypanosoma cruzi), DNA replication licensing factor MCM8, putative
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