Detailed view for TcCLB.504147.160

Basic information

TDR Targets ID: 34563
Trypanosoma cruzi, ADP-ribosylation factor-like protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.934 | Length (AA): 179 | MW (Da): 20514 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00025   ADP-ribosylation factor family

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0005525   GTP binding  
GO:0015031   protein transport  
GO:0007264   small GTPase mediated signal transduction  
GO:0006886   intracellular protein transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 178 1moz (A) 2 179 35.00 0 1 1.55 -1.49
4 177 1fzq (A) 3 177 39.00 0 1 1.61 -1.58
16 176 2a5d (A) 12 172 36.00 0 1 1.53 -1.82
1 179 3lrp (A) 1 179 35.00 0.00000032 1 1.57 -1.47
2 176 1zj6 (A) 2 175 31.00 0 1 1.48515 -1.44
3 177 1fzq (A) 2 177 39.00 0.0000015 1 1.58165 -1.41
16 179 1upt (A) 16 179 37.00 0 1 1.5436 -1.69
22 60 2ihy (B) 33 70 53.00 0.39 0.41 0.691277 0.66

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_129025)

Species Accession Gene Product
Arabidopsis thaliana AT3G49870   ADP-ribosylation factor-like A1C
Arabidopsis thaliana AT5G67560   ADP-ribosylation factor-like A1D
Arabidopsis thaliana AT5G37680   ADP-ribosylation factor-like A1A
Brugia malayi Bm1_39420   small GTP-binding protein domain containing protein
Caenorhabditis elegans CELE_Y57G11C.13   Protein ARL-8
Dictyostelium discoideum DDB_G0283525   ADP-ribosylation factor-like protein
Drosophila melanogaster Dmel_CG7891   GTPase indispensable for equal segregation of chromosomes
Echinococcus granulosus EgrG_000569800   ADP ribosylation factor protein 8B
Echinococcus multilocularis EmuJ_000569800   ADP ribosylation factor protein 8B
Homo sapiens ENSG00000134108   ADP-ribosylation factor-like 8B
Homo sapiens ENSG00000143862   ADP-ribosylation factor-like 8A
Loa Loa (eye worm) LOAG_01924   small GTP-binding protein domain-containing protein
Mus musculus ENSMUSG00000026426   ADP-ribosylation factor-like 8A
Mus musculus ENSMUSG00000030105   ADP-ribosylation factor-like 8B
Oryza sativa 4331969   Os03g0200800
Oryza sativa 4330631   Os02g0732500
Schistosoma japonicum Sjp_0042820   ko:K07956 ADP-ribosylation factor-like 8B, putative
Schistosoma mansoni Smp_166930   ADP-ribosylation factor-like 10 arl10
Schmidtea mediterranea mk4.001522.06   ADP-ribosylation factor-like protein 8B
Schmidtea mediterranea mk4.000431.13  
Trypanosoma cruzi TcCLB.504147.160   ADP-ribosylation factor-like protein, putative
Trypanosoma cruzi TcCLB.508897.70   ADP-ribosylation factor family, putative
Trichomonas vaginalis TVAG_502030   ADP-ribosylation factor, arf, putative
Trichomonas vaginalis TVAG_246190   GTP-binding protein sar1, putative
Trichomonas vaginalis TVAG_267180   GTP-binding protein sar1, putative
Trichomonas vaginalis TVAG_062590   ADP-ribosylation factor, arf, putative

Essentiality

TcCLB.504147.160 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_Y57G11C.13 Caenorhabditis elegans embryonic lethal wormbase
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.504147.160 (Trypanosoma cruzi), ADP-ribosylation factor-like protein, putative
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