Detailed view for PF3D7_0303800

Basic information

TDR Targets ID: 3538
Plasmodium falciparum, IBR domain protein, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.0019 | Length (AA): 593 | MW (Da): 71873 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01485   IBR domain, a half RING-finger domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008270   zinc ion binding  
GO:0004842   ubiquitin-protein ligase activity  
GO:0016567   protein ubiquitination  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
89 592 4kbl (A) 101 551 23.00 0.000000072 1 0.732116 1.35
131 575 4kc9 (A) 121 526 29.00 0 1 0.715922 1.74
286 397 5aiu (A) 136 255 28.00 0.97 1 0.32737 1.21
420 590 4uos (A) 3 165 13.00 0.6 0.05 0.446864 -0.96
553 584 2ytf (A) 8 40 3.00 0.00054 0 -0.0538371 0.85
87 592 4kbl (A) 99 551 19.00 0 1 0.656688 1.75
281 381 2m48 (A) 353 471 37.00 0.0000056 0.18 -0.0414796 2.49
420 590 4uos (A) 3 165 12.00 0.2 0.03 0.428764 -0.89
553 584 2emg (A) 8 40 9.00 0.048 0.02 -0.0161371 1.12

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, early trophozoite, late schizont, late trophozoite. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, gametocyte, early schizont, late ring, Sporozoite. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Oocyst, Ring, Female Gametocyte, Male Gametocyte. Zanghi G Lasonder E
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_126891)

Species Accession Gene Product
Arabidopsis thaliana AT2G31760   putative E3 ubiquitin-protein ligase ARI10
Arabidopsis thaliana AT2G31770   putative E3 ubiquitin-protein ligase ARI9
Arabidopsis thaliana AT2G16090   putative E3 ubiquitin-protein ligase ARI2
Arabidopsis thaliana AT3G27710   putative E3 ubiquitin-protein ligase ARI3
Arabidopsis thaliana AT1G65430   putative E3 ubiquitin-protein ligase ARI8
Arabidopsis thaliana AT1G05890   putative E3 ubiquitin-protein ligase ARI5
Arabidopsis thaliana AT2G31780   putative E3 ubiquitin-protein ligase ARI11
Arabidopsis thaliana AT2G31510   putative E3 ubiquitin-protein ligase ARI7
Arabidopsis thaliana AT3G27720   IBR domain containing protein
Arabidopsis thaliana AT4G34370   putative E3 ubiquitin-protein ligase ARI1
Brugia malayi Bm1_36320   Ariadne-2 protein
Brugia malayi Bm1_07850   ubiquitin-conjugating enzyme E2-binding protein 1, putative
Candida albicans CaO19.7224   potential IBR-type zinc finger protein similar to S. cerevisiae YKR017C
Caenorhabditis elegans CELE_C27A12.6   Protein C27A12.6
Caenorhabditis elegans CELE_Y73F8A.34   Protein TAG-349, isoform B
Caenorhabditis elegans CELE_C27A12.7   Protein C27A12.7, isoform B
Caenorhabditis elegans CELE_T12E12.1   Protein T12E12.1
Caenorhabditis elegans CELE_C27A12.8   Protein ARI-1
Dictyostelium discoideum DDB_G0286961   C3HC4-type zinc finger-containing protein
Dictyostelium discoideum DDB_G0275145   hypothetical protein
Dictyostelium discoideum DDB_G0278981   TRIAD1 protein
Drosophila melanogaster Dmel_CG5709   ariadne 2
Drosophila melanogaster Dmel_CG5659   ariadne
Drosophila melanogaster Dmel_CG12362   CG12362 gene product from transcript CG12362-RB
Echinococcus granulosus EgrG_001177850   protein ariadne 2
Echinococcus granulosus EgrG_000057300   Zinc finger C6HC type
Entamoeba histolytica EHI_096780   zinc finger protein, putative
Entamoeba histolytica EHI_134790   zinc finger protein, putative
Entamoeba histolytica EHI_122760   zinc finger protein, putative
Echinococcus multilocularis EmuJ_001177850   protein ariadne 2
Echinococcus multilocularis EmuJ_000057300   Zinc finger, C6HC type
Homo sapiens ENSG00000177479   ariadne RBR E3 ubiquitin protein ligase 2
Homo sapiens 25820   ariadne RBR E3 ubiquitin protein ligase 1
Leishmania braziliensis LbrM.30.2120   hypothetical protein, conserved
Leishmania donovani LdBPK_302180.1   E3 ubiquitin-protein ligase, putative
Leishmania infantum LinJ.30.2180   hypothetical protein, conserved
Leishmania major LmjF.30.2170   hypothetical protein, conserved
Leishmania mexicana LmxM.29.2170   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_04897   ariadne-2 protein
Loa Loa (eye worm) LOAG_08307   hypothetical protein
Mus musculus ENSMUSG00000064145   ariadne homolog 2 (Drosophila)
Mus musculus ENSMUSG00000025234   ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1 (Drosophila)
Neospora caninum NCLIV_050310   hypothetical protein
Oryza sativa 4332164   Os03g0233500
Oryza sativa 4346186   Os08g0540300
Oryza sativa 4336253   Os04g0492100
Oryza sativa 4347840   Os09g0559100
Oryza sativa 4347561   Os09g0513800
Oryza sativa 4347839   Os09g0559000
Plasmodium berghei PBANKA_0402400   IBR domain protein, putative
Plasmodium falciparum PF3D7_0303800   IBR domain protein, putative
Plasmodium knowlesi PKNH_0839300   IBR domain protein, putative
Plasmodium vivax PVX_119315   hypothetical protein, conserved
Plasmodium yoelii PY00504   putative IBR domain protein
Saccharomyces cerevisiae YKR017C   Hel1p
Schistosoma japonicum Sjp_0027660   ko:K01931 ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1 [EC:6.3.2.19], putative
Schistosoma japonicum Sjp_0219810   ko:K01931 ariadne 2 [EC:6.3.2.19], putative
Schistosoma mansoni Smp_130600   ariadne-1 protein homolog (ari-1) (ubiquitin-conjugating enzyme E2-binding protein 1)
Schistosoma mansoni Smp_150890   ariadne-2 protein homolog (ari-2)
Schmidtea mediterranea mk4.000681.09   Probable protein ariadne-2
Schmidtea mediterranea mk4.009160.01  
Schmidtea mediterranea mk4.008683.00   Putative ariadne-1 protein homolog
Trypanosoma brucei gambiense Tbg972.6.3550   hypothetical protein, conserved
Trypanosoma brucei Tb927.6.3780   E3 ubiquitin-protein ligase, putative
Trypanosoma congolense TcIL3000_0_04540   RING finger protein, putative
Trypanosoma cruzi TcCLB.511745.30   E3 ubiquitin-protein ligase, putative
Toxoplasma gondii TGME49_235980   ARIADNE family protein
Trichomonas vaginalis TVAG_074660   ariadne RING finger, putative
Trichomonas vaginalis TVAG_373620   ankyrin repeat and ibr domain containing protein, putative
Trichomonas vaginalis TVAG_074680   ariadne RING finger, putative

Essentiality

PF3D7_0303800 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.3780 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.3780 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.3780 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.3780 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0402400 Plasmodium berghei Dispensable plasmo
TGME49_235980 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_0303800 (Plasmodium falciparum), IBR domain protein, putative
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