Detailed view for TcCLB.510089.130

Basic information

TDR Targets ID: 35920
Trypanosoma cruzi, CMGC/CLK family protein kinase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.8708 | Length (AA): 625 | MW (Da): 70542 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 11 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
111 622 2eu9 (A) 137 473 26.00 0 1 0.39 0.84
129 388 1csn () 11 179 18.00 0.000026 0.94 -0.08 1.32
309 620 1wak (A) 176 652 35.00 0 0.99 0.58 -0.11
449 554 1t4h (B) 305 481 9.00 0.000044 0.02 0.25 -0.27
125 620 3llt (A) 547 873 32.00 0 1 0.5421 1.23
128 170 5uyj (A) 163 205 30.00 0 0.81 0.3472 0.07
179 359 3pg1 (A) 6 172 32.00 0.0036 1 0.4211 0.6
309 620 2x7g (A) 188 696 35.00 0 1 0.5027 0.28
328 359 4eqm (A) 116 147 56.00 0.016 0.99 0.6437 0.48
459 534 4nw6 (A) 231 305 29.00 0.1 0.64 0.4611 -0.44
582 620 2bva (A) 533 571 46.00 0.53 0.57 0.5039 0.57

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_148710)

Species Accession Gene Product
Leishmania braziliensis LbrM.25.2530   protein kinase, putative
Leishmania donovani LdBPK_251620.1   protein kinase, putative
Leishmania infantum LinJ.25.1620   protein kinase, putative
Leishmania major LmjF.25.1560   protein kinase, putative
Leishmania mexicana LmxM.25.1560   protein kinase, putative
Trypanosoma brucei gambiense Tbg972.3.1410   protein kinase, putative
Trypanosoma brucei Tb927.3.1610   CMGC/CLK family protein kinase, putative
Trypanosoma congolense TcIL3000_3_710   protein kinase, putative
Trypanosoma congolense TcIL3000_0_16890   protein kinase, putative
Trypanosoma cruzi TcCLB.510089.130   CMGC/CLK family protein kinase, putative
Trypanosoma cruzi TcCLB.510421.150   CMGC/CLK family protein kinase, putative

Essentiality

TcCLB.510089.130 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.1610 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.1610 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.1610 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.3.1610 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0022 0.5 0.5
0.0059 1 1
0.0063 0.7244 0.2543
0.0064 0.3377 0
0.0056 1 0.5
0.0081 1 0.5
0.0088 0.4477 0.5
0.0093 0.8828 0
0.0032 0.5 0.5
0.0037 1 0.5
0.0039 0.5 0.5
0.0032 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0012 0.5 0.5
0.0033 1 0.5
0.0016 0.5 0.5
0.0012 0.5 0.5
0.0007 0.5 0.5
0.0091 1 0.5
0.0081 0.5 0.5
0.0004 0.5 0.5
0.0033 0.5 0.5
0.0063 1 0.5
0.0007 0.5 0.5
0.0018 0.5 0.5
0.0042 0.5 0.5
0.0059 1 1
0.0069 0.3067 1
0.0066 0.3101 0
0.0067 0.5 0.5
0.0016 0.5 0.5
0.0027 1 0.5
0.0061 0.6883 0.5304
0.0036 0.5 0.5
0.0039 0.5 0.5
0.0026 0.5 0.5
0.0029 0.5 0.5
0.0011 1 0.5
0.0092 1 0.5
0.0008 0.5 0.5
0.0098 0.3242 0.3649
0.0039 0.9485 0.5
0.0023 0.5 0.5
0.0007 0.5 0.5
0.0059 1 1
0.0062 0.6935 0

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TcCLB.510089.130 (Trypanosoma cruzi), CMGC/CLK family protein kinase, putative
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