Detailed view for PF3D7_0309300

Basic information

TDR Targets ID: 3593
Plasmodium falciparum, N2227-like protein, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 5.1032 | Length (AA): 955 | MW (Da): 112248 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02363   Cysteine rich repeat
PF07942   N2227-like protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0003899   DNA-directed RNA polymerase activity  
GO:0003677   DNA binding  
GO:0006350   transcription  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
302 434 2ahx (A) 112 237 29.00 0.11 1 0.473367 -0.75
353 431 1uha (A) 4 82 27.00 0.37 0.65 0.499823 -1.93
394 736 4kc9 (A) 161 551 11.00 0.011 0.01 0.366062 -0.25
425 498 3u9u (E) 144 237 28.00 0.85 1 0.568587 -1.92
14 133 4ux7 (A) 64 184 32.00 0.18 0.09 0.170251 0.58
302 430 2ahx (A) 112 237 28.00 0.11 0.97 0.506777 -0.9
383 435 1uha (A) 29 82 34.00 0.55 0.83 0.570888 -2.37
387 467 1ulk (A) 29 123 32.00 0.022 0.94 0.458637 -1.61
390 460 3l95 (Y) 1464 1552 35.00 0.0096 0.62 0.458941 -1.85

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 16 hs, Ring. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, merozoite, sporozoite, early ring, early trophozoite, late ring, late schizont. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile gametocyte, early schizont, late trophozoite, Oocyst, Sporozoite, Male Gametocyte. PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Female Gametocyte. Lasonder E
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_128180)

Species Accession Gene Product
Arabidopsis thaliana AT2G32160   hypothetical protein
Arabidopsis thaliana AT2G32170   S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Babesia bovis BBOV_III006290   N2227-like family protein
Brugia malayi Bm1_56640   RIKEN cDNA 2410127L17
Candida albicans CaO19.592   putative methyltransferase protein
Candida albicans CaO19.8224   putative methyltransferase protein
Caenorhabditis elegans CELE_Y48E1C.2   Protein Y48E1C.2
Cryptosporidium hominis Chro.40162   hypothetical protein
Cryptosporidium parvum cgd4_1420   hypothetical protein
Dictyostelium discoideum DDB_G0282239   N2227-like domain-containing protein
Drosophila melanogaster Dmel_CG11596   CG11596 gene product from transcript CG11596-RA
Echinococcus granulosus EgrG_001133300   N2227
Entamoeba histolytica EHI_011930   hypothetical protein
Echinococcus multilocularis EmuJ_001133300   N2227
Homo sapiens ENSG00000156017   chromosome 9 open reading frame 41
Leishmania braziliensis LbrM.20.0990   hypothetical protein, conserved
Leishmania donovani LdBPK_341090.1   Carnosine N-methyltransferase, putative
Leishmania infantum LinJ.34.1090   hypothetical protein, conserved
Leishmania major LmjF.34.1020   hypothetical protein, conserved
Leishmania mexicana LmxM.33.1020   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_09552   hypothetical protein
Mus musculus ENSMUSG00000024726   RIKEN cDNA 2410127L17 gene
Mus musculus 102638361   UPF0586 protein C9orf41 homolog
Neospora caninum NCLIV_017180   hypothetical protein
Oryza sativa 4339287   Os05g0511300
Onchocerca volvulus OVOC10277  
Plasmodium berghei PBANKA_0407500   N2227-like protein, putative
Plasmodium falciparum PF3D7_0309300   N2227-like protein, putative
Plasmodium knowlesi PKNH_0833400   N2227-like protein, putative
Plasmodium vivax PVX_119580   hypothetical protein, conserved
Plasmodium yoelii PY03955   hypothetical protein
Saccharomyces cerevisiae YNL092W   hypothetical protein
Schistosoma japonicum Sjp_0210410   similar to UPF0586 protein C1778.07, putative
Schistosoma mansoni Smp_160530   hypothetical protein
Schmidtea mediterranea mk4.007533.00   UPF0586 protein C9orf41
Schmidtea mediterranea mk4.007533.03   UPF0586 protein C9orf41
Schmidtea mediterranea mk4.003681.01   UPF0586 protein C9orf41
Schmidtea mediterranea mk4.003681.00   UPF0586 protein C9orf41
Trypanosoma brucei gambiense Tbg972.4.3360   hypothetical protein, conserved
Trypanosoma brucei Tb927.4.3350   Carnosine N-methyltransferase, putative
Trypanosoma congolense TcIL3000_4_3050   Carnosine N-methyltransferase, putative
Trypanosoma cruzi TcCLB.506435.430   Carnosine N-methyltransferase, putative
Trypanosoma cruzi TcCLB.507739.70   N2227-like protein, putative
Toxoplasma gondii TGME49_241150   hypothetical protein
Theileria parva TP02_0368   hypothetical protein, conserved
Trichomonas vaginalis TVAG_246220   protein C9orf41, putative

Essentiality

PF3D7_0309300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.3350 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.3350 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.3350 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.3350 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0407500 Plasmodium berghei Slow plasmo
TGME49_241150 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_0309300 (Plasmodium falciparum), N2227-like protein, putative
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