Detailed view for TcCLB.511693.70

Basic information

TDR Targets ID: 36011
Trypanosoma cruzi, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.0306 | Length (AA): 630 | MW (Da): 67873 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00400   WD domain, G-beta repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 626 2b5l (A) 437 1046 12.00 0 1 0.95 0.8
12 146 1erj (A) 335 519 26.00 0.00000095 1 0.56 -0.84
31 622 1nr0 (A) 27 609 13.00 0 1 1.1 -0.04
1 622 4ci8 (A) 210 814 23.00 0 1 1.1697 0.29
319 579 3dm0 (A) 1055 1323 25.00 0.0000000021 1 0.685686 -0.1
430 507 4i79 (A) 222 344 35.00 0.011 0.49 0.42821 0.26
514 622 4u1e (I) 16 127 23.00 0.26 1 0.535416 -1.01

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_129802)

Species Accession Gene Product
Drosophila melanogaster Dmel_CG10064   CG10064 gene product from transcript CG10064-RA
Echinococcus granulosus EgrG_000626400   WD repeat containing protein 16
Echinococcus multilocularis EmuJ_000626400   WD repeat containing protein 16
Giardia lamblia GL50803_15218   WD-40 repeat protein
Homo sapiens ENSG00000166596   cilia and flagella associated protein 52
Leishmania braziliensis LbrM.28.1190   hypothetical protein, conserved
Leishmania donovani LdBPK_281210.1   WD domain, G-beta repeat, putative
Leishmania infantum LinJ.28.1210   hypothetical protein, conserved
Leishmania major LmjF.28.1110   hypothetical protein, conserved
Leishmania mexicana LmxM.28.1110   hypothetical protein, conserved
Mus musculus ENSMUSG00000020904   cilia and flagella associated protein 52
Neospora caninum NCLIV_027990   WD domain, G-beta repeat-containing protein, putative
Plasmodium berghei PBANKA_1361500   WD repeat-containing protein 16, putative
Plasmodium falciparum PF3D7_1348700   WD repeat-containing protein 16, putative
Plasmodium knowlesi PKNH_1252200   WD repeat-containing protein 16, putative
Plasmodium vivax PVX_083330   hypothetical protein, conserved
Plasmodium yoelii PY05114   hypothetical protein
Plasmodium yoelii PY05113   hypothetical protein
Schistosoma japonicum Sjp_0101630   WD repeat-containing protein 16, putative
Schistosoma mansoni Smp_134200   hypothetical protein
Schmidtea mediterranea mk4.013128.00  
Schmidtea mediterranea mk4.013128.01   Novel protein
Schmidtea mediterranea mk4.010372.00  
Trypanosoma brucei gambiense Tbg972.11.8760   hypothetical protein, conserved,predicted WD40 repeat protein
Trypanosoma brucei Tb927.11.7560   Component of motile flagella 15
Trypanosoma congolense TcIL3000.11.8170   predicted WD40 repeat protein
Trypanosoma cruzi TcCLB.511693.70   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.508177.10   hypothetical protein, conserved
Toxoplasma gondii TGME49_258530   flagellar associated protein
Trichomonas vaginalis TVAG_030160   F-box and WD domain protein, putative

Essentiality

TcCLB.511693.70 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.5550 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.5550 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.5550 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.5550 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1361500 Plasmodium berghei Dispensable plasmo
TGME49_258530 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier TcCLB.511693.70 (Trypanosoma cruzi), hypothetical protein, conserved
Title for this comment
Comment