Detailed view for PF3D7_0311400

Basic information

TDR Targets ID: 3613
Plasmodium falciparum, protein kinase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.9511 | Length (AA): 2515 | MW (Da): 295497 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 13 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
78 504 2etr (A) 8 404 20.00 0 1 0.06 -0.05
125 490 1rdq (E) 2 350 22.00 0 1 0.09 -0.55
133 518 2etr (A) 46 405 23.00 0 1 0.34 -0.16
158 456 2bik (B) 35 305 24.00 0 1 0.38 -1.02
12 546 4tnd (A) 26 543 16.00 0 1 0.287524 0.48
109 437 4btf (A) 145 451 20.00 0.2 0.99 0.100115 0.18
117 583 3uzt (A) 149 639 17.00 0.000000000028 0.84 0.0369859 1.21
146 443 1vzo (A) 34 320 24.00 0 1 0.355289 -0.24
148 619 3q5i (A) 48 504 25.00 0 1 0.257974 0.38
167 477 4yno (A) 31 340 24.00 0 1 0.165458 0.31
271 441 4ix3 (A) 295 488 16.00 0.29 0.86 0.0937921 0.23
744 1062 2fds (A) 17 315 28.00 0.91 0.66 0.095639 0.7
1693 1901 4tql (A) 10 224 22.00 0.35 0.38 0.348601 -0.89

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Female Gametocyte, Male Gametocyte. Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile gametocyte. PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Sporozoite. Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile late trophozoite, Oocyst. PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, Ring. Otto TD Zanghi G
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_152950)

Species Accession Gene Product
Cryptosporidium hominis Chro.70144   protein kinase
Cryptosporidium parvum cgd7_1190   Ser/Thr protein kinase
Neospora caninum NCLIV_061730   PIK3R4 kinase-related protein, putative
Plasmodium berghei PBANKA_0409400   protein kinase, putative
Plasmodium falciparum PF3D7_0311400   protein kinase, putative
Plasmodium yoelii PY02490   putative protein kinase
Toxoplasma gondii TGME49_218550   PIK3R4 kinase-related protein

Essentiality

PF3D7_0311400 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0409400 Plasmodium berghei Dispensable plasmo
TGME49_218550 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Bos taurus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 25.4% 287 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0062 0.6935 0
0.0059 1 1
0.0039 0.9485 0.5
0.0098 0.3242 0.2614
0.0007 0.5 0.5
0.0023 0.5 0.5
0.0029 0.5 0.5
0.0092 1 0.5
0.0011 1 0.5
0.0008 0.5 0.5
0.0026 0.5 0.5
0.0039 0.5 0.5
0.0061 0.6883 1
0.0036 0.5 0.5
0.0016 0.5 0.5
0.0067 0.5 0.5
0.0027 1 0.5
0.0066 0.3101 0
0.0069 0.3067 1
0.0059 1 1
0.0018 0.5 0.5
0.0042 0.5 0.5
0.0007 0.5 0.5
0.0063 1 1
0.0033 0.5 0.5
0.0081 0.5 0.5
0.0091 1 0.5
0.0004 0.5 0.5
0.0007 0.5 0.5
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0033 1 1
0.0012 0.5 0.5
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0039 0.5 0.5
0.0032 0.5 0.5
0.0037 1 0.5
0.0032 0.5 0.5
0.0093 0.8828 0
0.0081 1 0.5
0.0088 0.4477 0.5
0.0056 1 0.5
0.0064 0.3377 0
0.0063 0.7244 0.7244
0.0022 0.5 0.5
0.0059 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier PF3D7_0311400 (Plasmodium falciparum), protein kinase, putative
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