Detailed view for PF3D7_1316000

Basic information

TDR Targets ID: 365
Plasmodium falciparum, protein kinase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.5087 | Length (AA): 328 | MW (Da): 38191 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0005524   ATP binding  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 300 1j1b (A) 35 345 27.00 0 1 1.09 0.06
4 297 1q8y (A) 148 708 26.00 0 0.99 1.25 -0.36
52 298 2exe (A) 198 475 22.00 0 0.93 0.97 -0.59
1 328 4oli (A) 866 1174 27.00 0 1 1.0363 0.66
14 327 4e7w (A) 38 325 33.00 0 1 1.26462 0.05
20 217 2f57 (A) 455 638 28.00 0.00000000043 0.19 0.646859 0.89
142 251 3vut (A) 227 329 42.00 0.095 0.15 -0.000434146 2.14
258 328 4mvf (A) 245 331 21.00 0.012 0.71 0.477663 -0.77

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile gametocyte, Female Gametocyte. PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile late ring, Male Gametocyte. PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 48 hs, Oocyst, Ring, Sporozoite. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 40 hs. Otto TD
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_147629)

Species Accession Gene Product
Cryptosporidium hominis Chro.20204   protein kinase
Cryptosporidium parvum cgd2_1880   protein kinase, putative
Neospora caninum NCLIV_039150   CMGC kinase, putative
Plasmodium berghei PBANKA_1414500   protein kinase, putative
Plasmodium falciparum PF3D7_1316000   protein kinase, putative
Plasmodium knowlesi PKNH_1416700   protein kinase, putative
Plasmodium vivax PVX_122625   protein kinase, putative
Plasmodium yoelii PY06554   MAP kinase-related
Toxoplasma gondii TGME49_266910   cell-cycle-associated protein kinase GSK, putative

Essentiality

PF3D7_1316000 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1414500 Plasmodium berghei Dispensable plasmo
TGME49_266910 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Cell division protein kinase 5 292 aa 28.0% 329 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 32.6% 178 aa Compounds References
Patiria pectinifera Cdc2 300 aa 26.5% 324 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 22.5% 289 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 27.4% 318 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 28.1% 327 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0056 1 0.5
0.0081 1 0.5
0.0088 0.4477 0.5
0.0032 0.5 0.5
0.0093 0.8828 0
0.0059 1 1
0.0022 0.5 0.5
0.0063 0.7244 0.7244
0.0064 0.3377 0
0.0033 1 1
0.0016 0.5 0.5
0.0012 0.5 0.5
0.0007 0.5 0.5
0.0037 1 0.5
0.0032 0.5 0.5
0.0039 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0012 0.5 0.5
0.0042 0.5 0.5
0.0018 0.5 0.5
0.0059 1 1
0.0069 0.3067 1
0.0004 0.5 0.5
0.0081 0.5 0.5
0.0091 1 0.5
0.0063 1 1
0.0033 0.5 0.5
0.0007 0.5 0.5
0.0008 0.5 0.5
0.0011 1 0.5
0.0029 0.5 0.5
0.0092 1 0.5
0.0023 0.5 0.5
0.0007 0.5 0.5
0.0039 0.9485 0.5
0.0098 0.3242 0.2614
0.0059 1 1
0.0062 0.6935 0
0.0066 0.3101 0
0.0027 1 0.5
0.0016 0.5 0.5
0.0067 0.5 0.5
0.0061 0.6883 1
0.0039 0.5 0.5
0.0036 0.5 0.5
0.0026 0.5 0.5

Assayability

Assay information

  • Assay for Protein Kinase C (2.7.1.37 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

180 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_1316000 (Plasmodium falciparum), protein kinase, putative
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