Detailed view for TcCLB.504157.90

Basic information

TDR Targets ID: 36569
Trypanosoma cruzi, NEDD8-activating enzyme E1 regulatory subunit, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.8085 | Length (AA): 586 | MW (Da): 66039 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00899   ThiF family

Gene Ontology

Mouse over links to read term descriptions.
GO:0019781   NEDD8 activating enzyme activity  
GO:0008641   small protein activating enzyme activity  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0045116   protein neddylation  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 249 1jw9 (B) 4 243 15.00 0 0.84 0.31 -0.59
5 565 1ngv (A) 12 524 32.00 0 1 1.31 -0.56
1 570 1tt5 (A) 8 529 31.00 0 1 1.2451 0.09
19 96 3kyc (B) 9 102 33.00 0.066 0.87 0.315706 0.21
21 160 3guc (A) 71 203 13.00 0 0.66 0.319908 -0.22
278 316 2l3g (A) 38 78 41.00 0.4 0.26 0.411153 0.28

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128412)

Species Accession Gene Product
Arabidopsis thaliana AT2G32410   AXR1-like protein
Arabidopsis thaliana AT1G05180   RUB-activating enzyme E1
Brugia malayi Bm1_02035   ThiF family protein
Candida albicans CaO19.6299   similar to S. pombe uba5 (SPAC323.06c) ubiquitin activating enzyme and to S. cerevisiae ULA1 (YPL003W)
Candida albicans CaO19.13678   similar to S. pombe uba5 (SPAC323.06c) ubiquitin activating enzyme and to S. cerevisiae ULA1 (YPL003W)
Candida albicans CaO19.11630   Required for activation of RUB1
Candida albicans CaO19.4153   Required for activation of RUB1
Caenorhabditis elegans CELE_C26E6.8   Protein ULA-1
Dictyostelium discoideum DDB_G0287965   NEDD8-activating enzyme E1 regulatory subunit
Drosophila melanogaster Dmel_CG7828   beta-Amyloid precursor protein binding protein 1
Echinococcus granulosus EgrG_000928200   NEDD8 activating enzyme E1 regulatory subunit
Entamoeba histolytica EHI_000900   ThiF family protein
Echinococcus multilocularis EmuJ_000928200   NEDD8 activating enzyme E1 regulatory subunit
Homo sapiens ENSG00000159593   NEDD8 activating enzyme E1 subunit 1
Leishmania braziliensis LbrM.33.2930   hypothetical protein, conserved
Leishmania donovani LdBPK_332790.1   hypothetical protein, conserved
Leishmania infantum LinJ.33.2790   hypothetical protein, conserved
Leishmania major LmjF.33.2650   hypothetical protein, conserved
Leishmania mexicana LmxM.32.2650   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_07183   hypothetical protein
Mus musculus ENSMUSG00000031878   NEDD8 activating enzyme E1 subunit 1
Neospora caninum NCLIV_033670   GF23890, related
Oryza sativa 4334591   Os03g0820100
Saccharomyces cerevisiae YPL003W   Ula1p
Schistosoma japonicum Sjp_0300910   ko:K04532 amyloid beta precursor protein binding protein 1, putative
Schistosoma mansoni Smp_087920   app binding protein
Schmidtea mediterranea mk4.003613.06   Putative app binding protein
Trypanosoma brucei gambiense Tbg.972.2.2220   ubiquitin activating enzyme, putative
Trypanosoma brucei Tb927.2.4020   NEDD8-activating enzyme E1 regulatory subunit, putative
Trypanosoma congolense TcIL3000_2_550   ubiquitin activating enzyme, putative
Trypanosoma congolense TcIL3000_0_39080   ubiquitin activating enzyme, putative
Trypanosoma cruzi TcCLB.504157.90   NEDD8-activating enzyme E1 regulatory subunit, putative
Toxoplasma gondii TGME49_274070   ThiF family protein
Trichomonas vaginalis TVAG_357810   ubiquitin-activating enzyme E1, putative
Trichomonas vaginalis TVAG_457880   ubiquitin-activating enzyme E1, putative

Essentiality

TcCLB.504157.90 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.2.4020 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.2.4020 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.2.4020 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.2.4020 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C26E6.8 Caenorhabditis elegans embryonic lethal wormbase
CELE_C26E6.8 Caenorhabditis elegans larval arrest wormbase
CELE_C26E6.8 Caenorhabditis elegans slow growth wormbase
CELE_C26E6.8 Caenorhabditis elegans sterile wormbase
TGME49_274070 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens NEDD8 activating enzyme E1 subunit 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.0045 0.4201 0.8811

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.504157.90 (Trypanosoma cruzi), NEDD8-activating enzyme E1 regulatory subunit, putative
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