Detailed view for TcCLB.508461.230

Basic information

TDR Targets ID: 36643
Trypanosoma cruzi, kinetoplastid kinetochore protein 3, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.6604 | Length (AA): 1078 | MW (Da): 120254 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 12 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 382 2etr (A) 30 404 18.00 0 1 0.2 0.14
2 320 1ymi (A) 5 324 20.00 0 1 0.54 -0.43
20 380 1kob (A) 38 361 20.00 0 1 0.44 -0.06
121 318 2b9h (A) 103 307 29.00 0.0000000079 1 0.49 -0.91
5 343 4ejn (A) 90 438 22.00 0 1 0.254871 0.67
5 244 5k5x (A) 562 904 30.00 0.000000046 1 0.371935 0.54
5 316 3g0e (A) 560 922 26.00 0 1 0.280825 0.16
31 312 3g2f (A) 199 495 19.00 0 1 0.413996 -0.15
31 501 5iso (A) 12 531 19.00 0 1 0.43632 0.78
35 332 5ax9 (A) 25 301 22.00 0 1 0.334838 -0.31
152 236 2dyl (A) 256 338 51.00 0.000042 0.12 0.0671497 1.27
693 752 5edv (A) 868 926 34.00 0.57 0.36 0.205959 1.29

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_151836)

Species Accession Gene Product
Leishmania braziliensis LbrM.34.4040   protein kinase, putative
Leishmania donovani LdBPK_354110.1   kinetoplastid kinetochore protein 3, putative
Leishmania infantum LinJ.35.4110   protein kinase, putative
Leishmania major LmjF.35.4050   protein kinase, putative
Leishmania mexicana LmxM.34.4050   protein kinase, putative
Trypanosoma brucei gambiense Tbg972.9.6430   protein kinase, putative
Trypanosoma brucei Tb927.9.10920   kinetoplastid kinetochore protein 3
Trypanosoma congolense TcIL3000_9_4440   kinetoplastid kinetochore protein 3, putative
Trypanosoma cruzi TcCLB.508461.230   kinetoplastid kinetochore protein 3, putative

Essentiality

TcCLB.508461.230 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.2260 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.2260 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.2260 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.2260 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Sus scrofa Casein kinase I isoform alpha 125 aa 30.1% 123 aa Compounds References
Xenopus laevis Aurora kinase B-A 361 aa 23.0% 304 aa Compounds References
Rattus norvegicus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 23.5% 294 aa Compounds References
Xenopus laevis Aurora kinase B-B 368 aa 23.0% 305 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0037 1 0.5
0.0032 0.5 0.5
0.0039 0.5 0.5
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0033 1 0.5
0.0012 0.5 0.5
0.0007 0.5 0.5
0.0059 1 1
0.0022 0.5 0.5
0.0063 0.7244 0.2543
0.0064 0.3377 0
0.0056 1 0.5
0.0081 1 0.5
0.0088 0.4477 0.5
0.0093 0.8828 0
0.0032 0.5 0.5
0.0066 0.3101 0
0.0027 1 0.5
0.0067 0.5 0.5
0.0016 0.5 0.5
0.0061 0.6883 0.5304
0.0036 0.5 0.5
0.0039 0.5 0.5
0.0026 0.5 0.5
0.0008 0.5 0.5
0.0029 0.5 0.5
0.0011 1 0.5
0.0092 1 0.5
0.0023 0.5 0.5
0.0007 0.5 0.5
0.0098 0.3242 0.3649
0.0039 0.9485 0.5
0.0059 1 1
0.0062 0.6935 0
0.0004 0.5 0.5
0.0081 0.5 0.5
0.0091 1 0.5
0.0033 0.5 0.5
0.0063 1 0.5
0.0007 0.5 0.5
0.0042 0.5 0.5
0.0018 0.5 0.5
0.0059 1 1
0.0069 0.3067 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.508461.230 (Trypanosoma cruzi), kinetoplastid kinetochore protein 3, putative
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