Detailed view for PF3D7_0323700

Basic information

TDR Targets ID: 3735
Plasmodium falciparum, U4/U6.U5 tri-snRNP-associated protein 1, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 5.0915 | Length (AA): 693 | MW (Da): 82900 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03343   SART-1 family

Gene Ontology

Mouse over links to read term descriptions.
GO:0000398   nuclear mRNA splicing, via spliceosome  

Metabolic Pathways

Spliceosome (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
11 122 2v66 (B) 58 168 11.00 0.83 0 0.381616 -1.84
38 141 2pms (C) 168 288 17.00 0.58 0.01 0.401072 -1.54
145 691 1j7n (B) 49 604 22.00 0.32 0.85 0.879822 0.68

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Oocyst, Sporozoite. Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, merozoite, sporozoite, early ring, early trophozoite, late ring, late schizont, Ring, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, early schizont, late trophozoite. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile gametocyte. PlasmoDB
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_128840)

Species Accession Gene Product
Arabidopsis thaliana AT3G14700   SART-1 family protein
Arabidopsis thaliana AT5G16780   SART-1 family protein DOT2
Babesia bovis BBOV_III007920   hypothetical protein
Brugia malayi Bm1_10910   SART-1 family protein
Candida albicans CaO19.4326   similar to spliceosomal U4/U6.U5 tri-snRNP protein
Candida albicans CaO19.11801   similar to spliceosomal U4/U6.U5 tri-snRNP protein
Caenorhabditis elegans CELE_F19F10.9   Protein F19F10.9
Cryptosporidium parvum cgd4_1570   hypothetical protein, possible conserved
Dictyostelium discoideum DDB_G0281621   SART-1 family protein
Drosophila melanogaster Dmel_CG6686   CG6686 gene product from transcript CG6686-RB
Echinococcus granulosus EgrG_000823100   hypoxia associated factor
Entamoeba histolytica EHI_135030   hypothetical protein
Echinococcus multilocularis EmuJ_000823100   hypoxia associated factor
Homo sapiens ENSG00000175467   squamous cell carcinoma antigen recognized by T cells
Loa Loa (eye worm) LOAG_07174   hypothetical protein
Mus musculus ENSMUSG00000039148   squamous cell carcinoma antigen recognized by T cells 1
Neospora caninum NCLIV_011340   Os02g0511500 protein, related
Oryza sativa 4329455   Os02g0511500
Plasmodium berghei PBANKA_1214800   U4/U6.U5 tri-snRNP-associated protein 1, putative
Plasmodium falciparum PF3D7_0323700   U4/U6.U5 tri-snRNP-associated protein 1, putative
Plasmodium knowlesi PKNH_0816700   U4/U6.U5 tri-snRNP-associated protein 1, putative
Plasmodium vivax PVX_095040   hypothetical protein, conserved
Plasmodium yoelii PY00360   SART-1 family
Saccharomyces cerevisiae YOR308C   Snu66p
Schistosoma japonicum Sjp_0017220   U4/U6.U5 tri-snRNP-associated protein 1, putative
Schistosoma mansoni Smp_176840   hypothetical protein
Schmidtea mediterranea mk4.012394.00  
Schmidtea mediterranea mk4.016715.00  
Toxoplasma gondii TGME49_318140   SART-1 family protein
Theileria parva TP04_0696   hypothetical protein, conserved
Trichomonas vaginalis TVAG_091900   conserved hypothetical protein

Essentiality

PF3D7_0323700 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_F19F10.9 Caenorhabditis elegans embryonic lethal wormbase
CELE_F19F10.9 Caenorhabditis elegans larval arrest wormbase
CELE_F19F10.9 Caenorhabditis elegans sterile wormbase
TGME49_318140 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier PF3D7_0323700 (Plasmodium falciparum), U4/U6.U5 tri-snRNP-associated protein 1, putative
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