Detailed view for TcCLB.509011.90

Basic information

TDR Targets ID: 38199
Trypanosoma cruzi, lipase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.7115 | Length (AA): 347 | MW (Da): 38763 | Paralog Number: 7

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01764   Lipase (class 3)

Gene Ontology

Mouse over links to read term descriptions.
GO:0004806   triacylglycerol lipase activity  
GO:0006629   lipid metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
21 292 1lgy (A) 8 266 31.00 0 1 1.10446 -0.71
27 329 3ngm (A) 9 317 31.00 0 1 1.191 -0.35
137 186 5jd6 (A) 72 114 44.00 0.042 0.63 0.404692 0.75

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127315)

Species Accession Gene Product
Arabidopsis thaliana AT5G67050   alpha/beta-Hydrolases superfamily protein
Arabidopsis thaliana AT5G18640   alpha/beta-Hydrolases superfamily protein
Arabidopsis thaliana AT5G18630   putative class 3 lipase
Brugia malayi Bm1_47735   Lipase family protein
Candida albicans CaO19.4678   potential triglyceride lipase similar to S. cerevisiae YJR107W
Candida albicans CaO19.7747   weak similarity to S. cerevisiae YJR107
Candida albicans CaO19.12147   potential triglyceride lipase similar to S. cerevisiae YJR107W
Candida albicans CaO19.100   weak similarity to S. cerevisiae YJR107
Caenorhabditis elegans CELE_F46G10.4   Protein F46G10.4
Caenorhabditis elegans CELE_T21H3.1   Protein T21H3.1, isoform B
Caenorhabditis elegans CELE_F58B6.1   Protein F58B6.1
Caenorhabditis elegans CELE_F25A2.1   Protein F25A2.1
Caenorhabditis elegans CELE_F28H7.3   Protein F28H7.3
Caenorhabditis elegans CELE_T10B5.7   Protein T10B5.7
Dictyostelium discoideum DDB_G0270934   hypothetical protein
Dictyostelium discoideum DDB_G0277473   hypothetical protein
Dictyostelium discoideum DDB_G0291394   hypothetical protein
Leishmania braziliensis LbrM.33.1730   lipase precursor-like protein
Leishmania braziliensis LbrM.31.2750   lipase, putative
Leishmania donovani LdBPK_312540.1   lipase, putative
Leishmania donovani LdBPK_310870.1   lipase precursor-like protein
Leishmania infantum LinJ.31.2540   lipase, putative
Leishmania infantum LinJ.31.0870   lipase precursor-like protein
Leishmania major LmjF.31.2460   lipase, putative
Leishmania major LmjF.31.0840   lipase precursor-like protein
Leishmania mexicana LmxM.30.2460   lipase, putative
Loa Loa (eye worm) LOAG_09088   lipase
Oryza sativa 4338595   Os05g0372400
Oryza sativa 9269259   Os03g0348800
Oryza sativa 4351045   Os11g0655800
Oryza sativa 4325371   Os01g0651800
Oryza sativa 4344227   Os07g0668700
Onchocerca volvulus OVOC1034  
Saccharomyces cerevisiae YJR107W   putative lipase
Trypanosoma brucei gambiense Tbg972.4.5160   lipase, putative
Trypanosoma brucei Tb927.8.7440   lipase, putative
Trypanosoma congolense TcIL3000_8_7680   lipase, putative
Trypanosoma congolense TcIL3000_8_7830   lipase, putative
Trypanosoma congolense TcIL3000_4_4200   lipase, putative
Trypanosoma cruzi TcCLB.509011.90   lipase, putative
Trypanosoma cruzi TcCLB.510679.100   lipase, putative
Trypanosoma cruzi TcCLB.510679.60   lipase, putative
Trypanosoma cruzi TcCLB.510679.70   lipase, putative
Trypanosoma cruzi TcCLB.510681.30   lipase, putative
Trypanosoma cruzi TcCLB.511437.30   lipase, putative
Trypanosoma cruzi TcCLB.511439.30   lipase, putative
Trypanosoma cruzi TcCLB.511439.20   lipase, putative

Essentiality

TcCLB.509011.90 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.7440 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.7440 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.7440 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.7440 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Malassezia globosa (strain ATCC MYA-4612 / CBS 7966)(Dandruff-associated fungus) LIP1, secretory lipase (Family 3) Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.509011.90 (Trypanosoma cruzi), lipase, putative
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