Detailed view for TcCLB.511903.30

Basic information

TDR Targets ID: 38628
Trypanosoma cruzi, ubiquitin-conjugating enzyme E2, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.6993 | Length (AA): 156 | MW (Da): 17428 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00179   Ubiquitin-conjugating enzyme

Gene Ontology

Mouse over links to read term descriptions.
GO:0019787   small conjugating protein ligase activity  
GO:0051246   regulation of protein metabolic process  
GO:0043687   post-translational protein modification  

Metabolic Pathways

Ubiquitin mediated proteolysis (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 154 2e2c () 22 175 54.00 0 1 1.8 -1.81
2 156 2cyx (A) 0 162 33.00 0 1 1.41 -1.39
12 153 1i7k (A) 33 174 58.00 0 1 1.78 -2.08
1 155 1x23 (A) 0 144 39.00 0 1 1.50479 -0.91
1 154 2e2c (A) 22 175 54.00 0 1 1.74078 -1.32
9 155 4yii (U) 30 172 59.00 0 1 1.71951 -1.38

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_129878)

Species Accession Gene Product
Arabidopsis thaliana AT3G20060   ubiquitin-conjugating enzyme E2 19
Arabidopsis thaliana AT1G50490   ubiquitin-conjugating enzyme E2 20
Dictyostelium discoideum DDB_G0278775   ubiquitin-conjugating enzyme E2
Drosophila melanogaster Dmel_CG10682   vihar
Echinococcus granulosus EgrG_000169200   ubiquitin conjugating enzyme E2 C
Echinococcus multilocularis EmuJ_000169200   ubiquitin conjugating enzyme E2 C
Leishmania braziliensis LbrM.24.2210   ubiquitin-conjugating enzyme e2, putative
Leishmania donovani LdBPK_242220.1   ubiquitin-conjugating enzyme E2, putative
Leishmania infantum LinJ.24.2220   ubiquitin-conjugating enzyme e2, putative
Leishmania major LmjF.24.2130   ubiquitin-conjugating enzyme e2, putative
Leishmania mexicana LmxM.24.2130   ubiquitin-conjugating enzyme e2, putative
Mus musculus ENSMUSG00000001403   ubiquitin-conjugating enzyme E2C
Oryza sativa 4325174   Os01g0273100
Saccharomyces cerevisiae YOR339C   putative E2 ubiquitin-protein ligase UBC11
Schistosoma mansoni Smp_051010   ubiquitin-conjugating enzyme E2 C
Trypanosoma brucei gambiense Tbg972.8.6120   ubiquitin-conjugating enzyme e2, putative,ubiquitin carrier protein, putative,ubiquitin-protein ligase, putative
Trypanosoma brucei Tb927.8.6090   ubiquitin-protein ligase, putative
Trypanosoma congolense TcIL3000_8_5870   ubiquitin-protein ligase, putative
Trypanosoma congolense TcIL3000_0_40960   ubiquitin-conjugating enzyme E2, putative
Trypanosoma cruzi TcCLB.511071.70   ubiquitin-conjugating enzyme E2, putative
Trypanosoma cruzi TcCLB.511903.30   ubiquitin-conjugating enzyme E2, putative

Essentiality

TcCLB.511903.30 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.6090 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.6090 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.6090 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.6090 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.511903.30 (Trypanosoma cruzi), ubiquitin-conjugating enzyme E2, putative
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