Detailed view for PF3D7_0416100

Basic information

TDR Targets ID: 3934
Plasmodium falciparum, glutamyl-tRNA(Gln) amidotransferase subunit A
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.6062 | Length (AA): 826 | MW (Da): 96150 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01425   Amidase

Gene Ontology

Mouse over links to read term descriptions.
GO:0050567   glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity  
GO:0020011   apicoplast  
GO:0004040   amidase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
169 810 1m22 (A) 43 522 22.00 0 1 0.59 0.87
208 403 1ock (A) 22 212 35.00 0 1 0.71 -0.59
175 818 4wj3 (A) 1 483 28.00 0 1 0.610361 1.55
208 416 4n0h (A) 7 226 38.00 0 1 0.709027 -0.58
217 393 4iss (A) 52 224 47.00 0 1 0.718286 -0.26
225 424 3ip4 (A) 46 255 46.00 0 1 0.776131 -0.56
173 803 1m22 (A) 47 529 21.00 0.0000023 1 0.593012 1.16
187 509 1o9p (A) 1 317 25.00 0.0066 1 0.691765 0.27
243 791 4wj3 (A) 69 472 36.00 0 1 0.610978 0.75

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Sporozoite. Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, early schizont, Oocyst, Female Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, late schizont, late trophozoite, Ring, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_127466)

Species Accession Gene Product
Arabidopsis thaliana AT3G25660   amidase family protein
Babesia bovis BBOV_III009760   amidase family protein
Brugia malayi Bm1_39735   Amidase family protein
Candida albicans CaO19.11438   likely amidase similar to S. cerevisiae YMR293C mitochondrial putative glutamyl-tRNA amidotransferase
Candida albicans CaO19.3956   likely amidase similar to S. cerevisiae YMR293C mitochondrial putative glutamyl-tRNA amidotransferase
Caenorhabditis elegans CELE_Y41D4A.6   Protein Y41D4A.6
Chlamydia trachomatis CT_003   glutamyl-tRNA(Gln) amidotransferase subunit A
Dictyostelium discoideum DDB_G0286875   glutamyl-tRNA amidotransferase A subunit
Drosophila melanogaster Dmel_CG6007   glutamyl-tRNA amidotransferase subunit A
Echinococcus granulosus EgrG_000232600   glutamyl tRNAGln amidotransferase subunit A
Echinococcus multilocularis EmuJ_000232600   glutamyl tRNA(Gln) amidotransferase subunit A
Homo sapiens ENSG00000130348   glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1
Loa Loa (eye worm) LOAG_00738   amidase
Mycobacterium leprae ML1702c   PROBABLE GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE (SUBUNIT A) GATA (Glu-ADT SUBUNIT A)
Mus musculus ENSMUSG00000019863   glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1
Mycobacterium tuberculosis Rv3011c   Probable glutamyl-tRNA(GLN) amidotransferase (subunit A) GatA (Glu-ADT subunit A)
Mycobacterium ulcerans MUL_1939   aspartyl/glutamyl-tRNA amidotransferase subunit A
Oryza sativa 4337177   Os04g0643200
Plasmodium berghei PBANKA_0718100   glutamyl-tRNA(Gln) amidotransferase subunit A
Plasmodium falciparum PF3D7_0416100   glutamyl-tRNA(Gln) amidotransferase subunit A
Plasmodium knowlesi PKNH_0508500   glutamyl-tRNA(Gln) amidotransferase subunit A, putative
Plasmodium vivax PVX_089895   glutamyl-tRNA(Gln) amidotransferase subunit A, putative
Plasmodium yoelii PY02858   Glu-tRNAGln amidotransferase subunit A, putative
Saccharomyces cerevisiae YMR293C   glutamyl-tRNA(Gln) amidotransferase subunit HER2
Schistosoma japonicum Sjp_0031480   ko:K02433 aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A, putative
Schistosoma mansoni Smp_031990   glutamyl-tRNA(Gln) amidotransferase subunit A
Schmidtea mediterranea mk4.006670.00   Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial
Schmidtea mediterranea mk4.003810.01   Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial
Treponema pallidum TP1020   aspartyl/glutamyl-tRNA amidotransferase subunit A
Theileria parva TP04_0894   glutamyl-tRNA amidotransferase subunit A, putative
Wolbachia endosymbiont of Brugia malayi Wbm0763   aspartyl/glutamyl-tRNA amidotransferase subunit A

Essentiality

PF3D7_0416100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0718100 Plasmodium berghei Slow plasmo
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_0416100 (Plasmodium falciparum), glutamyl-tRNA(Gln) amidotransferase subunit A
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