Detailed view for TcCLB.511355.7

Basic information

TDR Targets ID: 39534
Trypanosoma cruzi, TATA-box-binding protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.3214 | Length (AA): 245 | MW (Da): 26692 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00352   Transcription factor TFIID (or TATA-binding protein, TBP)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005669   transcription factor TFIID complex  
GO:0005634   nucleus  
GO:0005488   binding  
GO:0003702   RNA polymerase II transcription factor activity  
GO:0003677   DNA binding  
GO:0006367   transcription initiation from RNA polymerase II promoter  
GO:0006352   transcription initiation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
22 245 4b0a (A) 11 322 31.00 0 1 1.27699 -0.13
77 245 1mp9 (A) 10 170 27.00 0 1 1.0057 0.02
77 245 3eik (A) 20 180 32.00 0 1 1.0897 -0.32
122 183 2ziu (B) 307 415 38.00 0.22 0.27 0.431461 1.42

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_126912)

Species Accession Gene Product
Arabidopsis thaliana AT1G55520   TATA-box-binding protein 2
Babesia bovis BBOV_IV002970   TATA-box binding protein
Brugia malayi Bm1_46430   TATA-box binding protein
Candida albicans CaO19.9395   potential TATA-binding protein similar to S. cerevisiae TFIID subunit SPT15 (YER148W)
Candida albicans CaO19.1837   potential TATA-binding protein similar to S. cerevisiae TFIID subunit SPT15 (YER148W)
Caenorhabditis elegans CELE_T20B12.2   Protein TBP-1
Cryptosporidium hominis Chro.80238   TATA-binding protein
Cryptosporidium parvum cgd8_2030   TATA-box factor binding protein
Dictyostelium discoideum DDB_G0275617   TATA-binding protein
Drosophila melanogaster Dmel_CG9874   TATA binding protein
Drosophila melanogaster Dmel_CG7562   TBP-related factor
Echinococcus granulosus EgrG_000419500   TATA box binding protein 2
Echinococcus granulosus EgrG_000972300   tata box binding protein
Entamoeba histolytica EHI_020610   transcription initiation factor TFIID family protein
Entamoeba histolytica EHI_112050   transcription initiation factor TFIID family protein
Entamoeba histolytica EHI_077240   transcription initiation factor TFIID family protein
Echinococcus multilocularis EmuJ_000419500   TATA box binding protein 2
Echinococcus multilocularis EmuJ_000972300   tata box binding protein
Giardia lamblia GL50803_1721   TATA-binding protein-like factor
Homo sapiens ENSG00000112592   TATA box binding protein
Homo sapiens 387332   TATA box binding protein like 2
Leishmania braziliensis LbrM.19.1660   transcription initiation factor-like protein (TFIID-like protein)
Leishmania donovani LdBPK_191430.1   TATA-box-binding protein
Leishmania infantum LinJ.19.1430   transcription initiation factor-like protein (TFIID-like protein)
Leishmania major LmjF.19.1390   transcription initiation factor-like protein (TFIID-like protein)
Leishmania mexicana LmxM.19.1390   transcription initiation factor-like protein (TFIID-like protein)
Loa Loa (eye worm) LOAG_01904   TATA-box binding protein
Mus musculus ENSMUSG00000061809   TATA box binding protein like 2
Mus musculus ENSMUSG00000014767   TATA box binding protein
Neospora caninum NCLIV_027830   TATA-binding protein, related
Oryza sativa 4352633   Os12g0580300
Oryza sativa 4333619   Os03g0657000
Oryza sativa 4348699   Os10g0432300
Plasmodium berghei PBANKA_1105800   TATA-box binding protein, putative
Plasmodium falciparum PF3D7_0506200   TATA-box-binding protein
Plasmodium knowlesi PKNH_1027400   TATA-box-binding protein, putative
Plasmodium vivax PVX_097865   TATA-box binding protein, putative
Plasmodium yoelii PY00712   transcription initiation factor tfiid
Saccharomyces cerevisiae YER148W   TATA-binding protein
Schistosoma japonicum Sjp_0304380   ko:K03120 transcription initiation factor TFIID TATA-box-binding protein, putative
Schistosoma japonicum Sjp_0040590   TATA-box-binding protein, putative
Schistosoma mansoni Smp_098950   TATA-box binding protein
Schistosoma mansoni Smp_073680.2   TATA-box binding protein
Schmidtea mediterranea mk4.000997.07   TATA-box-binding protein
Schmidtea mediterranea mk4.000388.09   TATA-box-binding protein
Schmidtea mediterranea mk4.001551.07   TBP-related factor
Schmidtea mediterranea mk4.000454.07   TATA-box-binding protein
Schmidtea mediterranea mk4.000838.01   TBP-related factor
Schmidtea mediterranea mk4.000454.03   TATA-box-binding protein
Schmidtea mediterranea mk4.001551.08   TBP-related factor
Schmidtea mediterranea mk4.002629.02   TBP-related factor
Schmidtea mediterranea mk4.000454.06   TATA-box-binding protein
Trypanosoma brucei gambiense Tbg972.10.19550   TFIID-like protein, putative
Trypanosoma brucei Tb927.10.15950   TATA-box-binding protein
Trypanosoma congolense TcIL3000_10_13790   TATA-box-binding protein
Trypanosoma cruzi TcCLB.511355.7   TATA-box-binding protein
Trypanosoma cruzi TcCLB.503809.149   TATA-box-binding protein
Toxoplasma gondii TGME49_258680   TATA-box binding protein TBP2
Theileria parva TP01_0630   transcription initiation factor TFIID, putative
Trichomonas vaginalis TVAG_291560   transcription initiation factor tfiid, putative
Trichomonas vaginalis TVAG_285070   transcription initiation factor tfiid, putative

Essentiality

TcCLB.511355.7 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.15950 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.15950 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.15950 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.15950 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T20B12.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_T20B12.2 Caenorhabditis elegans larval arrest wormbase
CELE_T20B12.2 Caenorhabditis elegans slow growth wormbase
YER148W Saccharomyces cerevisiae inviable yeastgenome
TGME49_258680 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.511355.7 (Trypanosoma cruzi), TATA-box-binding protein
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