Detailed view for PF3D7_0422700

Basic information

TDR Targets ID: 3997
Plasmodium falciparum, eukaryotic initiation factor 4A-III, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 7.5663 | Length (AA): 390 | MW (Da): 44800 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00270   DEAD/DEAH box helicase
PF00271   Helicase conserved C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016281   eukaryotic translation initiation factor 4F complex  
GO:0004004   ATP-dependent RNA helicase activity  
GO:0008026   ATP-dependent helicase activity  
GO:0005524   ATP binding  
GO:0004386   helicase activity  
GO:0003743   translation initiation factor activity  
GO:0003676   nucleic acid binding  
GO:0006446   regulation of translational initiation  

Metabolic Pathways

RNA transport (KEGG)
mRNA surveillance pathway (KEGG)
Spliceosome (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 390 1z6a (A) 2 899 14.00 0 1 0.69 0.34
6 390 1fuu (B) 11 393 52.00 0 1 1.67 -1.58
19 218 1vec (A) 87 287 35.00 0 1 1.19 -2.38
6 390 4c9b (A) 27 411 66.00 0 1 1.86248 -1.4
19 215 2oxc (A) 64 261 38.00 0 1 1.15463 -1.87

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, Oocyst, Ring. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 32 hs, merozoite, sporozoite, early ring, early schizont, early trophozoite, late ring, late trophozoite, Male Gametocyte. Otto TD PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, gametocyte, Female Gametocyte. Otto TD PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Sporozoite. Zanghi G
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_127927)

Species Accession Gene Product
Arabidopsis thaliana AT3G19760   DEAD-box ATP-dependent RNA helicase 2
Babesia bovis BBOV_IV010990   eukaryotic initiation factor 4A-3 (eIF4A-3), putative
Brugia malayi Bm1_14510   ATP-dependent helicase DDX48
Candida albicans CaO19.2488   DEAD-box protein, putative RNA helicase similar to S. cerevisiae FAL1 (YDR021W) involved in rRNA processing
Candida albicans CaO19.10024   DEAD-box protein, putative RNA helicase similar to S. cerevisiae FAL1 (YDR021W) involved in rRNA processing
Caenorhabditis elegans CELE_Y65B4A.6   Protein Y65B4A.6
Caenorhabditis elegans CELE_F33D11.10   Protein F33D11.10
Cryptosporidium hominis Chro.70440   eukaryotic initiation factor 4A-3 (eIF4A-3) (eIF-4A-3)
Cryptosporidium parvum cgd7_3940   eIF4A-1; eukaryotic translation initiation factor 4A-1; RNA SFII helicase
Dictyostelium discoideum DDB_G0269192   DEAD/DEAH box helicase domain-containing protein
Drosophila melanogaster Dmel_CG7483   CG7483 gene product from transcript CG7483-RA
Echinococcus granulosus EgrG_001193600   eukaryotic initiation factor 4A III
Echinococcus granulosus EgrG_000033400   DEAD box ATP dependent RNA helicase
Entamoeba histolytica EHI_106300   DEAD/DEAH box helicase, putative
Echinococcus multilocularis EmuJ_000371200   DEAD box ATP dependent RNA helicase
Echinococcus multilocularis EmuJ_001193600   eukaryotic initiation factor 4A III
Echinococcus multilocularis EmuJ_000033400   DEAD box ATP dependent RNA helicase
Homo sapiens ENSG00000141543   eukaryotic translation initiation factor 4A3
Leishmania braziliensis LbrM.28.1700   DEAD box RNA helicase, putative
Leishmania donovani LdBPK_281660.1   DEAD box RNA helicase, putative
Leishmania infantum LinJ.28.1660   DEAD box RNA helicase, putative
Leishmania major LmjF.28.1530   ATP dependent DEAD-box helicase, putative
Leishmania mexicana LmxM.28.1530   DEAD box RNA helicase, putative
Loa Loa (eye worm) LOAG_07474   ATP-dependent helicase DDX48
Mus musculus 668137   predicted gene 8994
Mus musculus ENSMUSG00000025580   eukaryotic translation initiation factor 4A3
Mus musculus 102641531   eukaryotic initiation factor 4A-III-like
Neospora caninum NCLIV_015210   ATP-dependent helicase, putaive, putative
Oryza sativa 4326580   Os01g0639100
Oryza sativa 4333273   Os03g0566800
Plasmodium berghei PBANKA_0523100   eukaryotic initiation factor 4A-III, putative
Plasmodium falciparum PF3D7_0422700   eukaryotic initiation factor 4A-III, putative
Plasmodium knowlesi PKNH_0514600   eukaryotic initiation factor 4A-III, putative
Plasmodium vivax PVX_090175   eukaryotic initiation factor, putative
Plasmodium yoelii PY06447   eukaryotic initiation factor 4a-3
Saccharomyces cerevisiae YDR021W   ATP-dependent RNA helicase FAL1
Schistosoma japonicum Sjp_0055030   ko:K03257 translation initiation factor eIF-4A, putative
Schistosoma mansoni Smp_034190.2   DEAD box ATP-dependent RNA helicase
Schistosoma mansoni Smp_034190.1   DEAD box ATP-dependent RNA helicase
Schmidtea mediterranea mk4.001215.00   Eukaryotic initiation factor 4A-III
Schmidtea mediterranea mk4.000568.02   Putative dead box ATP-dependent RNA helicase
Trypanosoma brucei gambiense Tbg972.11.9800   ATP-dependent DEAD/H RNA helicase, putative,DEAD box RNA helicase, putative,RNA helicase, putative
Trypanosoma brucei Tb927.11.8770   ATP-dependent RNA helicase FAL1, putative
Trypanosoma congolense TcIL3000.11.9080   ATP-dependent RNA helicase FAL1, putative
Trypanosoma cruzi TcCLB.506587.40   ATP-dependent RNA helicase FAL1, putative
Toxoplasma gondii TGME49_256770   eukaryotic translation initiation factor 4A, isoform 3, putative
Theileria parva TP01_0765   eukaryotic translation initiation factor 4A, putative
Trichomonas vaginalis TVAG_286500   DEAD box ATP-dependent RNA helicase, putative

Essentiality

PF3D7_0422700 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.12.0011 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.12.0011 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.12.0011 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.12.0011 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F33D11.10 Caenorhabditis elegans embryonic lethal wormbase
CELE_F33D11.10 Caenorhabditis elegans larval arrest wormbase
CELE_F33D11.10 Caenorhabditis elegans larval lethal wormbase
CELE_F33D11.10 Caenorhabditis elegans slow growth wormbase
CELE_F33D11.10 Caenorhabditis elegans sterile wormbase
CELE_Y65B4A.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y65B4A.6 Caenorhabditis elegans larval arrest wormbase
CELE_Y65B4A.6 Caenorhabditis elegans slow growth wormbase
CELE_Y65B4A.6 Caenorhabditis elegans sterile wormbase
YDR021W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0523100 Plasmodium berghei Essential plasmo
TGME49_256770 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_0422700 (Plasmodium falciparum), eukaryotic initiation factor 4A-III, putative
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