Detailed view for TcCLB.506985.40

Basic information

TDR Targets ID: 39970
Trypanosoma cruzi, Polyamine transporter 1, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.4767 | Length (AA): 627 | MW (Da): 67164 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 12

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13520   Amino acid permease

Gene Ontology

Mouse over links to read term descriptions.
GO:0022857   transmembrane transporter activity  
GO:0016020   membrane  
GO:0055085   transmembrane transport  
GO:0006810   transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
56 483 2a65 (A) 12 508 11.00 0 0.03 0.7 -1.79
19 128 1kpl (A) 265 382 33.00 0.38 0.12 0.245639 0.52
52 467 3gia (A) 7 418 14.00 0 0.69 0.889477 -0.74
85 485 4dji (A) 41 476 22.00 0.0000000062 0.49 0.698753 0.8
195 312 4mes (A) 35 155 28.00 0.011 0.02 0.0533978 1.77

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_130748)

Species Accession Gene Product
Arabidopsis thaliana AT3G19553   polyamine uptake transporter 5
Arabidopsis thaliana AT1G31830   polyamine transporter 2
Arabidopsis thaliana AT5G05630   polyamine uptake transporter 3
Arabidopsis thaliana AT1G31820   polyamine transporter 1
Dictyostelium discoideum DDB_G0267504   hypothetical protein
Entamoeba histolytica EHI_100280   amino acid-polyamine transporter, putative
Giardia lamblia GL50803_16433   Amino acid permease, putative
Leishmania braziliensis LbrM.34.4390   amino acid permease, putative
Leishmania braziliensis LbrM.14.0320   amino acid permease, putative
Leishmania donovani LdBPK_354480.1   amino acid permease, putative
Leishmania donovani LdBPK_140320.1   Polyamine transporter 1, putative
Leishmania infantum LinJ.35.4480   amino acid permease, putative
Leishmania infantum LinJ.14.0320   amino acid permease, putative
Leishmania major LmjF.14.0320   amino acid permease, putative
Leishmania major LmjF.35.4410   amino acid permease, putative
Leishmania mexicana LmxM.14.0320   amino acid permease, putative
Leishmania mexicana LmxM.34.4410   amino acid permease, putative
Oryza sativa 4333307   Os03g0576900
Oryza sativa 4330433   Os02g0700500
Trypanosoma brucei gambiense Tbg972.9.6000   amino acid transporter, putative
Trypanosoma brucei Tb927.9.10340   Polyamine transporter 1, putative
Trypanosoma congolense TcIL3000_9_3900   Polyamine transporter 1, putative
Trypanosoma cruzi TcCLB.506985.40   Polyamine transporter 1, putative
Trypanosoma cruzi TcCLB.504213.110   Putrescine-cadaverine transporter
Trypanosoma cruzi TcCLB.509551.20   Polyamine transporter 1, putative

Essentiality

TcCLB.506985.40 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.1760 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.1760 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.1760 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.1760 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Tcru008175AAC;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tcru008175; Recombinant protein: truncated; Source: T cruzi; amino acid permease, putative or peroxisomal 3-oxoacyl-CoA thiolase, putative ;

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.506985.40 (Trypanosoma cruzi), Polyamine transporter 1, putative
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