Detailed view for TcCLB.506553.30

Basic information

TDR Targets ID: 40536
Trypanosoma cruzi, GTP-binding protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.6642 | Length (AA): 499 | MW (Da): 54935 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01018   GTP1/OBG
PF01926   50S ribosome-binding GTPase

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0005525   GTP binding  
GO:0003924   GTPase activity  
GO:0000287   magnesium ion binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
44 479 1udx (A) 2 411 33.00 0 1 0.88 0.35
44 371 1udx (A) 2 312 42.00 0 1 1.13 -0.12
202 385 2cxx (A) 2 185 17.00 0 0.35 0.34 -0.95
44 461 1udx (A) 2 395 34.00 0 1 1.13418 0.57
44 390 1lnz (A) 2 340 39.00 0 1 1.06089 0.48
199 399 2e87 (A) 165 349 24.00 0 1 0.651906 -0.3
201 493 3k53 (A) 3 267 18.00 0.000052 0.89 0.726674 0.13

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127170)

Species Accession Gene Product
Arabidopsis thaliana AT1G07615   GTP-binding protein Obg/CgtA
Arabidopsis thaliana AT5G18570   OBGC-like protein
Babesia bovis BBOV_III000430   hypothetical protein
Babesia bovis BBOV_IV004510   GTPase family protein
Brugia malayi Bm1_49205   GTP1/OBG family protein
Candida albicans CaO19.13974   similar to S. cerevisiae YHR168W
Candida albicans CaO19.6653   similar to S. cerevisiae YHR168W
Caenorhabditis elegans CELE_M01E5.2   Protein M01E5.2
Chlamydia trachomatis CT_418   GTPase Obg
Dictyostelium discoideum DDB_G0277801   GTP-binding protein 5
Drosophila melanogaster Dmel_CG13390   CG13390 gene product from transcript CG13390-RA
Escherichia coli b3183   GTPase involved in cell partioning and DNA repair
Echinococcus granulosus EgrG_000665900   GTP binding protein 5
Echinococcus multilocularis EmuJ_000665900   GTP binding protein 5
Homo sapiens ENSG00000101181   mitochondrial ribosome-associated GTPase 2
Leishmania braziliensis LbrM.35.3210   GTP-binding protein, putative
Leishmania donovani LdBPK_363140.1   GTP-binding protein, putative
Leishmania infantum LinJ.36.3140   GTP-binding protein, putative
Leishmania major LmjF.36.2990   GTP-binding protein, putative
Leishmania mexicana LmxM.36.2990   GTP-binding protein, putative
Loa Loa (eye worm) LOAG_01749   GTP1/OBG family protein
Mycobacterium leprae ML1465c   PROBABLE GTP1/OBG-FAMILY GTP-BINDING PROTEIN OBG
Mus musculus ENSMUSG00000039069   mitochondrial ribosome associated GTPase 2
Mycobacterium tuberculosis Rv2440c   Probable GTP1/Obg-family GTP-binding protein Obg
Mycobacterium ulcerans MUL_3712   GTPase ObgE
Neospora caninum NCLIV_058650   hypothetical protein
Oryza sativa 4351211   Os11g0704300
Oryza sativa 4344232   Os07g0669200
Onchocerca volvulus OVOC13428  
Plasmodium berghei PBANKA_1030900   GTP-binding protein, putative
Plasmodium falciparum PF3D7_1411600   GTP-binding protein, putative
Plasmodium knowlesi PKNH_1346700   GTP-binding protein, putative
Plasmodium vivax PVX_085875   GTP-binding protein, putative
Plasmodium yoelii PY00161   GTP-binding protein-related
Saccharomyces cerevisiae YHR168W   putative GTPase MTG2
Schistosoma mansoni Smp_191360   mitochondrial gtpase
Schmidtea mediterranea mk4.004678.01   Mitochondrial ribosome-associated GTPase 2
Trypanosoma brucei gambiense Tbg972.10.9110   GTP-binding protein, putative
Trypanosoma brucei Tb927.10.7430   GTP-binding protein, putative
Trypanosoma congolense TcIL3000_10_6400   GTP-binding protein, putative
Trypanosoma cruzi TcCLB.507769.40   GTP-binding protein, putative
Trypanosoma cruzi TcCLB.506553.30   GTP-binding protein, putative
Toxoplasma gondii TGME49_316120   hypothetical protein
Treponema pallidum TP0742   GTPase ObgE
Theileria parva TP01_0299   hypothetical protein
Theileria parva TP03_0795   hypothetical protein
Wolbachia endosymbiont of Brugia malayi Wbm0120   GTPase ObgE

Essentiality

TcCLB.506553.30 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.7430 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.7430 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.7430 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.7430 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b3183 Escherichia coli essential goodall
TGME49_316120 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.506553.30 (Trypanosoma cruzi), GTP-binding protein, putative
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