Detailed view for TcCLB.510045.30

Basic information

TDR Targets ID: 40866
Trypanosoma cruzi, NAK family pseudokinase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.559 | Length (AA): 683 | MW (Da): 75974 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 11 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
61 507 1omw (A) 189 611 18.00 0 1 0.69 0.6
68 337 1t4h (A) 226 480 23.00 0 1 0.7 -0.9
20 303 4aw2 (A) 61 307 31.00 0.00035 0.97 0.542913 0.47
26 331 5vcz (A) 0 354 21.00 0 1 0.592523 0.12
58 426 4l9i (A) 182 522 19.00 0 1 0.639764 0.27
91 335 4c57 (A) 64 314 30.00 0 1 0.709812 -0.54
93 282 4im0 (A) 35 221 39.00 0.0000000051 0.97 0.443284 0.59
110 324 5hu3 (A) 60 260 26.00 0.0006 1 0.613888 -0.72
151 324 2y7j (A) 115 279 30.00 0.00000016 1 0.496858 -0.55
167 289 4eqm (A) 107 217 40.00 0.00013 0.13 0.187188 0.73
475 654 3pzd (A) 1516 1680 10.00 0 0.05 0.263743 -0.65

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_140422)

Species Accession Gene Product
Entamoeba histolytica EHI_158080   protein kinase, putative
Leishmania braziliensis LbrM.15.0790   protein kinase, putative
Leishmania donovani LdBPK_150810.1   protein kinase, putative
Leishmania infantum LinJ.15.0810   protein kinase, putative
Leishmania major LmjF.15.0770   protein kinase, putative
Leishmania mexicana LmxM.15.0770   protein kinase, putative
Trypanosoma brucei gambiense Tbg972.9.3470   protein kinase, putative
Trypanosoma brucei Tb927.9.6560   NAK family pseudokinase, putative
Trypanosoma congolense TcIL3000_9_2280   protein kinase, putative
Trypanosoma cruzi TcCLB.510045.30   NAK family pseudokinase, putative
Trypanosoma cruzi TcCLB.504883.10   NAK family pseudokinase, putative

Essentiality

TcCLB.510045.30 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.4770 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.160.4770 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.160.4770 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.160.4770 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.4

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 27.9% 190 aa Compounds References
Bos taurus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 23.5% 307 aa Compounds References
Rattus norvegicus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 23.5% 307 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0007 0.5 0.5
0.0081 0.5 0.5
0.0091 1 0.5
0.0004 0.5 0.5
0.0063 1 0.5
0.0033 0.5 0.5
0.0059 1 1
0.0069 0.3067 1
0.0018 0.5 0.5
0.0042 0.5 0.5
0.0061 0.6883 0.5304
0.0039 0.5 0.5
0.0036 0.5 0.5
0.0026 0.5 0.5
0.0066 0.3101 0
0.0016 0.5 0.5
0.0067 0.5 0.5
0.0027 1 0.5
0.0059 1 1
0.0062 0.6935 0
0.0029 0.5 0.5
0.0011 1 0.5
0.0092 1 0.5
0.0008 0.5 0.5
0.0098 0.3242 0.3649
0.0039 0.9485 0.5
0.0007 0.5 0.5
0.0023 0.5 0.5
0.0064 0.3377 0
0.0022 0.5 0.5
0.0059 1 1
0.0063 0.7244 0.2543
0.0093 0.8828 0
0.0032 0.5 0.5
0.0056 1 0.5
0.0088 0.4477 0.5
0.0081 1 0.5
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0037 1 0.5
0.0039 0.5 0.5
0.0032 0.5 0.5
0.0007 0.5 0.5
0.0016 0.5 0.5
0.0033 1 0.5
0.0012 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.510045.30 (Trypanosoma cruzi), NAK family pseudokinase, putative
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