Detailed view for TcCLB.505843.40

Basic information

TDR Targets ID: 41008
Trypanosoma cruzi, E1-like ubiquitin-activating enzyme, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.077 | Length (AA): 393 | MW (Da): 43046 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00899   ThiF family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016491   oxidoreductase activity  
GO:0008641   small protein activating enzyme activity  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 356 1ngv (B) 12 441 17.00 0 1 0.55 0.24
17 271 1zud (1) 4 240 27.00 0.0000000000031 1 0.98 -1.19
4 289 3dbh (B) 12 359 22.00 0 1 0.615335 0.15
18 202 1tt5 (A) 9 177 19.00 0 0.9 0.613338 -0.2
37 301 5iaa (A) 68 339 57.00 0 1 1.3489 -0.89

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_130165)

Species Accession Gene Product
Arabidopsis thaliana AT1G05350   NAD(P)-binding Rossmann-fold superfamily protein
Babesia bovis BBOV_III005200   ThiF family protein
Brugia malayi Bm1_36180   CG1749-PA
Caenorhabditis elegans CELE_T03F1.1   Protein T03F1.1
Cryptosporidium hominis Chro.40221   hypothetical protein
Cryptosporidium parvum cgd4_1960   ThiF/moeB family
Dictyostelium discoideum DDB_G0293306   E1-like enzyme family protein
Drosophila melanogaster Dmel_CG1749   CG1749 gene product from transcript CG1749-RA
Echinococcus granulosus EgrG_000471100   ubiquitin modifier activating enzyme 5
Echinococcus multilocularis EmuJ_000471100   ubiquitin modifier activating enzyme 5
Homo sapiens ENSG00000081307   ubiquitin-like modifier activating enzyme 5
Leishmania braziliensis LbrM.15.1010   NAD/FAD dependent dehydrogenase, putative
Leishmania donovani LdBPK_151030.1   NAD/FAD dependent dehydrogenase, putative
Leishmania infantum LinJ.15.1030   NAD/FAD dependent dehydrogenase, putative
Leishmania major LmjF.15.0970   NAD/FAD dependent dehydrogenase, putative
Leishmania mexicana LmxM.15.0970   NAD/FAD dependent dehydrogenase, putative
Loa Loa (eye worm) LOAG_02702   ubiquitin-activating enzyme 5
Mus musculus ENSMUSG00000032557   ubiquitin-like modifier activating enzyme 5
Neospora caninum NCLIV_031950   thiF family domain-containing protein, putative
Oryza sativa 4329438   Os02g0506500
Schistosoma japonicum Sjp_0311700   Ubiquitin-like modifier-activating enzyme 5, putative
Schistosoma japonicum Sjp_0096390   Ubiquitin-like modifier-activating enzyme 5, putative
Schistosoma japonicum Sjp_0216860   expressed protein
Schistosoma mansoni Smp_045780   ubiquitin-activating enzyme E1-related
Schmidtea mediterranea mk4.003153.02   Ubiquitin-like modifier-activating enzyme 5
Trypanosoma brucei gambiense Tbg972.9.3130   NAD/FAD dependent dehydrogenase, putative,ubiquitin-activating enzyme, putative
Trypanosoma brucei Tb927.9.6040   E1-like ubiquitin-activating enzyme, putative
Trypanosoma congolense TcIL3000_9_2040   E1-like ubiquitin-activating enzyme, putative
Trypanosoma congolense TcIL3000_0_07900   E1-like ubiquitin-activating enzyme, putative
Trypanosoma cruzi TcCLB.505843.40   E1-like ubiquitin-activating enzyme, putative
Trypanosoma cruzi TcCLB.509353.10   E1-like ubiquitin-activating enzyme, putative
Toxoplasma gondii TGME49_231940   ThiF family protein
Theileria parva TP02_0433   hypothetical protein, conserved

Essentiality

TcCLB.505843.40 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.4430 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.4430 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.4430 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.160.4430 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_231940 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.505843.40 (Trypanosoma cruzi), E1-like ubiquitin-activating enzyme, putative
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