Detailed view for TcCLB.510351.40

Basic information

TDR Targets ID: 41321
Trypanosoma cruzi, ATP-dependent RNA helicase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.9318 | Length (AA): 895 | MW (Da): 101555 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00270   DEAD/DEAH box helicase
PF00271   Helicase conserved C-terminal domain
PF13959   Domain of unknown function (DUF4217)

Gene Ontology

Mouse over links to read term descriptions.
GO:0008026   ATP-dependent helicase activity  
GO:0005524   ATP binding  
GO:0004386   helicase activity  
GO:0003676   nucleic acid binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
45 707 2eyq (A) 509 1147 12.00 0 1 0.45 0.48
71 490 2db3 (A) 203 619 26.00 0 1 0.66 -1.3
116 650 1oyw (A) 1 514 17.00 0 1 0.52 -0.21
118 323 1vec (A) 86 288 33.00 0 1 0.84 -2.27
117 325 2pl3 (A) 69 277 51.00 0 1 1.01172 -1.74
118 812 2p6r (A) 2 684 15.00 0 1 0.747736 0.89
122 320 3ly5 (A) 179 383 43.00 0 1 0.872546 -1.55
677 856 4uos (A) 1 183 13.00 0.25 0.12 0.396517 -0.95
774 867 1q6h (A) 32 140 28.00 0.41 0.01 0.215228 0.15

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_128229)

Species Accession Gene Product
Arabidopsis thaliana AT5G54910   DEAD-box ATP-dependent RNA helicase 32
Babesia bovis BBOV_II005490   DEAD/DEAH box helicase family protein
Brugia malayi Bm1_14335   DEAD/DEAH box helicase family protein
Candida albicans CaO19.2712   Probable RNA helicase
Candida albicans CaO19.10227   Probable RNA helicase
Caenorhabditis elegans CELE_Y23H5B.6   Protein Y23H5B.6
Cryptosporidium hominis Chro.60264   DEAD/DEAH box helicase
Cryptosporidium parvum cgd6_2260   Hca4p helicase DBP4 (helicase CA4). EIF4A-1-family RNA SFII helicase
Dictyostelium discoideum DDB_G0284017   DEAD/DEAH box helicase
Drosophila melanogaster Dmel_CG5800   CG5800 gene product from transcript CG5800-RA
Echinococcus granulosus EgrG_001058400   ATP dependent RNA helicase DDX10
Entamoeba histolytica EHI_119620   DEAD/DEAH box helicase, putative
Echinococcus multilocularis EmuJ_001058400   ATP dependent RNA helicase DDX10
Homo sapiens ENSG00000178105   DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
Leishmania braziliensis LbrM.28.1410   ATP-dependent RNA helicase, putative
Leishmania donovani LdBPK_281420.1   ATP-dependent RNA helicase, putative
Leishmania infantum LinJ.28.1420   ATP-dependent RNA helicase, putative
Leishmania major LmjF.28.1310   ATP-dependent RNA helicase, putative
Leishmania mexicana LmxM.28.1310   ATP-dependent RNA helicase, putative
Loa Loa (eye worm) LOAG_00217   hypothetical protein
Mus musculus ENSMUSG00000053289   DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
Neospora caninum NCLIV_018600   DEAD/DEAH box helicase, putative
Oryza sativa 4343385   Os07g0517000
Onchocerca volvulus OVOC11857  
Plasmodium berghei PBANKA_1024300   ATP-dependent RNA helicase DBP4, putative
Plasmodium falciparum PF3D7_1418900   ATP-dependent RNA helicase DBP4, putative
Plasmodium knowlesi PKNH_1339100   ATP-dependent RNA helicase DBP4, putative
Plasmodium vivax PVX_085535   DEAD/DEAH box ATP-dependent RNA helicase, putative
Plasmodium yoelii PY06632   RNA helicase, putative
Saccharomyces cerevisiae YJL033W   RNA-dependent ATPase HCA4
Schistosoma japonicum Sjp_0083690   ko:K01529 RNA Helicase [EC:3.6.1.-], putative
Schmidtea mediterranea mk4.001026.08  
Trypanosoma brucei gambiense Tbg972.5.5770   ATP-dependent DEAD/H RNA helicase, putative
Trypanosoma brucei Tb927.5.4270   ATP-dependent DEAD/H RNA helicase, putative
Trypanosoma congolense TcIL3000_5_4890   ATP-dependent DEAD/H RNA helicase, putative
Trypanosoma cruzi TcCLB.510351.40   ATP-dependent RNA helicase, putative
Trypanosoma cruzi TcCLB.507601.140   ATP-dependent RNA helicase, putative
Toxoplasma gondii TGME49_244000   DEAD/DEAH box helicase domain-containing protein
Theileria parva TP02_0498   ATP-dependent RNA helicase, putative
Trichomonas vaginalis TVAG_009310   DEAD box ATP-dependent RNA helicase, putative

Essentiality

TcCLB.510351.40 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.4270 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.5.4270 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.4270 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.4270 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y23H5B.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y23H5B.6 Caenorhabditis elegans larval arrest wormbase
CELE_Y23H5B.6 Caenorhabditis elegans slow growth wormbase
CELE_Y23H5B.6 Caenorhabditis elegans sterile wormbase
YJL033W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1024300 Plasmodium berghei Essential plasmo
TGME49_244000 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.510351.40 (Trypanosoma cruzi), ATP-dependent RNA helicase, putative
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