Detailed view for TcCLB.503819.30

Basic information

TDR Targets ID: 42436
Trypanosoma cruzi, eukaryotic translation initiation factor 2 subunit, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.0131 | Length (AA): 477 | MW (Da): 52580 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00009   Elongation factor Tu GTP binding domain
PF09173   Initiation factor eIF2 gamma, C terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0005525   GTP binding  
GO:0003924   GTPase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
48 470 1kk1 (A) 6 410 43.00 0 1 1.36369 -0.4
50 469 1s0u (A) 35 436 44.00 0 1 1.3204 -0.02
371 475 4zgn (B) 423 524 41.00 0 1 0.793926 -0.9

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127447)

Species Accession Gene Product
Arabidopsis thaliana AT1G04170   translation initiation factor eIF-2 gamma subunit
Arabidopsis thaliana AT2G18720   translation elongation factor EF1A/initiation factor IF2gamma family protein
Arabidopsis thaliana AT4G18330   putative translation elongation factor 2EF1A / eIF-2-gamma
Babesia bovis BBOV_IV004190   translation initiation factor 2 gamma subunit, putative
Brugia malayi Bm1_48910   eIF2 gamma
Candida albicans CaO19.11699   similar to S. cerevisiae GCD11 (YER025W) translation initiation factor eIF2 gamma subunit
Candida albicans CaO19.4223   similar to S. cerevisiae GCD11 (YER025W) translation initiation factor eIF2 gamma subunit
Caenorhabditis elegans CELE_Y39G10AR.8   Protein Y39G10AR.8
Cryptosporidium hominis Chro.30199   translation initiation factor eIF-2
Cryptosporidium parvum cgd3_1650   eIF2G GTpase. eukaryotic translation initiation factor 2 gamma subunit (ZnR+GTpase)
Dictyostelium discoideum DDB_G0278967   translation initiation factor 2 gamma
Drosophila melanogaster Dmel_CG43665   Eukaryotic initiation factor 2gamma
Echinococcus granulosus EgrG_000490900   eukaryotic translation initiation
Entamoeba histolytica EHI_132880   eukaryotic translation initiation factor 2 gamma subunit, putative
Echinococcus multilocularis EmuJ_000490900   eukaryotic translation initiation
Giardia lamblia GL50803_2970   Translation initiation factor 2 gamma subunit
Homo sapiens 1968   eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa
Leishmania braziliensis LbrM.09.1130   eukaryotic translation initiation factor 2 subunit, putative
Leishmania infantum LinJ.09.1130   eukaryotic translation initiation factor 2 subunit, putative
Leishmania major LmjF.09.1070   eukaryotic translation initiation factor 2 subunit, putative
Leishmania mexicana LmxM.09.1070   eukaryotic translation initiation factor 2 subunit, putative
Loa Loa (eye worm) LOAG_03629   hypothetical protein
Mus musculus 102642679   eukaryotic translation initiation factor 2 subunit 3-like
Mus musculus ENSMUSG00000035150   eukaryotic translation initiation factor 2, subunit 3, structural gene X-linked
Mus musculus ENSMUSG00000069049   eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked
Neospora caninum NCLIV_050300   GH18750, related
Oryza sativa 4352748   Os12g0607100
Oryza sativa 4351657   Os12g0176800
Onchocerca volvulus OVOC904   Eukaryotic translation initiation factor 2 subunit 3 homolog
Plasmodium berghei PBANKA_1031900   eukaryotic translation initiation factor 2 subunit gamma, putative
Plasmodium falciparum PF3D7_1410600   eukaryotic translation initiation factor 2 subunit gamma, putative
Plasmodium knowlesi PKNH_1347700   eukaryotic translation initiation factor 2 subunit gamma, putative
Plasmodium vivax PVX_085920   eukaryotic translation initiation factor 2 gamma subunit, putative
Plasmodium yoelii PY04028   translation initiation factor eIF-2
Saccharomyces cerevisiae YER025W   translation initiation factor eIF2 subunit gamma
Schistosoma japonicum Sjp_0219200   ko:K03242 translation initiation factor eIF-2 gamma subunit, putative
Schistosoma mansoni Smp_095150   eukaryotic translation initiation factor 2 gamma subunit (eif-2-gamma)
Schmidtea mediterranea mk4.000013.24   Eukaryotic translation initiation factor 2 subunit 3
Schmidtea mediterranea mk4.001673.00   Eukaryotic translation initiation factor 2 subunit 3
Trypanosoma brucei gambiense Tbg972.11.14790   eukaryotic translation initiation factor 2 gamma, putative
Trypanosoma brucei Tb927.11.13250   eukaryotic translation initiation factor 2 gamma, putative
Trypanosoma congolense TcIL3000.11.13610   eukaryotic translation initiation factor 2 gamma, putative
Trypanosoma cruzi TcCLB.510359.310   eukaryotic translation initiation factor 2 subunit, putative
Trypanosoma cruzi TcCLB.503819.30   eukaryotic translation initiation factor 2 subunit, putative
Toxoplasma gondii TGME49_235970   eukaryotic initiation factor-2 gamma, putative
Theileria parva TP01_0335   eukaryotic translation initiation factor 2 gamma subunit, putative
Trichomonas vaginalis TVAG_423490   eukaryotic translation initiation factor 2 gamma subunit, putative

Essentiality

TcCLB.503819.30 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.4830 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.4830 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.4830 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.4830 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y39G10AR.8 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y39G10AR.8 Caenorhabditis elegans larval arrest wormbase
CELE_Y39G10AR.8 Caenorhabditis elegans slow growth wormbase
CELE_Y39G10AR.8 Caenorhabditis elegans sterile wormbase
YER025W Saccharomyces cerevisiae inviable yeastgenome
TGME49_235970 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier TcCLB.503819.30 (Trypanosoma cruzi), eukaryotic translation initiation factor 2 subunit, putative
Title for this comment
Comment