Detailed view for TcCLB.510741.229

Basic information

TDR Targets ID: 42447
Trypanosoma cruzi, ubiquitin-activating enzyme E1, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.8152 | Length (AA): 411 | MW (Da): 45132 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00899   ThiF family
PF16191   Ubiquitin-activating enzyme E1 four-helix bundle

Gene Ontology

Mouse over links to read term descriptions.
GO:0008641   small protein activating enzyme activity  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  
GO:0006464   protein modification process  

Metabolic Pathways

Ubiquitin mediated proteolysis (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
65 411 1ngv (A) 6 463 15.00 0 1 0.45 0.56
65 295 1jw9 (B) 3 243 19.00 0 1 0.71 -1.12
9 160 4eqs (A) 54 213 30.00 0.16 0.66 0.43933 1.67
62 411 5l6h (A) 10 328 33.00 0 1 1.10608 0.66
71 328 1y8q (A) 16 339 28.00 0 1 0.800937 -0.57

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_145106)

Species Accession Gene Product
Leishmania braziliensis LbrM.34.2970   ubiquitin-activating enzyme e1, putative
Leishmania donovani LdBPK_353110.1   ubiquitin-activating enzyme E1, putative
Leishmania infantum LinJ.35.3110   ubiquitin-activating enzyme e1, putative
Leishmania major LmjF.35.3060   ubiquitin-activating enzyme e1, putative
Leishmania mexicana LmxM.34.3060   ubiquitin-activating enzyme e1, putative
Trypanosoma brucei gambiense Tbg972.9.7720   ubiquitin-activating enzyme e1, putative
Trypanosoma brucei Tb11.v5.0675   ubiquitin-activating enzyme E1, putative
Trypanosoma brucei Tb927.9.12650   ubiquitin-activating enzyme E1, putative
Trypanosoma congolense TcIL3000_0_55160   ubiquitin-activating enzyme E1, putative
Trypanosoma cruzi TcCLB.510741.229   ubiquitin-activating enzyme E1, putative

Essentiality

TcCLB.510741.229 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.3610 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.3610 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.3610 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.211.3610 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.510741.229 (Trypanosoma cruzi), ubiquitin-activating enzyme E1, putative
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