Detailed view for LbrM.09.0300

Basic information

TDR Targets ID: 427771
Leishmania braziliensis, protein kinase, putative
Source Database / ID: 

pI: 8.6234 | Length (AA): 883 | MW (Da): 94670 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
446 873 3gc9 (B) 2 340 28.00 0 1 0.411963 1.07
575 696 2rku (A) 137 251 34.00 0.0000000022 0.99 0.534209 -0.39
586 696 4oth (A) 718 821 43.00 0.00017 1 0.0657656 0.88
606 689 2qlu (A) 313 396 35.00 0.1 0.43 0.303223 0.43

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_151464)

Species Accession Gene Product
Leishmania braziliensis LbrM.09.0300   protein kinase, putative
Leishmania donovani LdBPK_090270.1   cdc2-related kinase 12
Leishmania infantum LinJ.09.0270   protein kinase, putative
Leishmania major LmjF.09.0310   protein kinase, putative
Leishmania mexicana LmxM.09.0310   protein kinase, putative
Trypanosoma brucei gambiense Tbg972.11.13750   protein kinase, putative
Trypanosoma brucei Tb927.11.12310   cdc2-related kinase 12
Trypanosoma congolense TcIL3000.11.12940   cdc2-related kinase 12
Trypanosoma cruzi TcCLB.511127.220   cdc2-related kinase 12
Trypanosoma cruzi TcCLB.509023.200   cdc2-related kinase 12

Essentiality

LbrM.09.0300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.4130 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.4130 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.4130 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.4130 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 30.6% 271 aa Compounds References
Rattus norvegicus c-Jun N-terminal kinase 3 464 aa 24.6% 394 aa Compounds References
Sus scrofa Casein kinase 1, delta tv1 415 aa 20.8% 341 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 29.5% 308 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 27.1% 376 aa Compounds References
Patiria pectinifera Cdc2 300 aa 32.6% 264 aa Compounds References
Schizosaccharomyces pombe 972h- Casein kinase II subunit alpha 332 aa 21.5% 409 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LbrM.09.0300 (Leishmania braziliensis), protein kinase, putative
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