Detailed view for TcCLB.511391.200

Basic information

TDR Targets ID: 42852
Trypanosoma cruzi, pitrilysin-like metalloprotease, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.4109 | Length (AA): 1024 | MW (Da): 114239 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00675   Insulinase (Peptidase family M16)
PF05193   Peptidase M16 inactive domain
PF08367   Peptidase M16C associated

Gene Ontology

Mouse over links to read term descriptions.
GO:0046872   metal ion binding  
GO:0008270   zinc ion binding  
GO:0008237   metallopeptidase activity  
GO:0004222   metalloendopeptidase activity  
GO:0003824   catalytic activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
15 1024 1q2l (A) 25 935 13.00 0 1 0.84 0.09
29 1024 2fge (A) 18 985 30.00 0 1 1.37 -0.91
32 1024 2fge (A) 21 981 26.00 0 1 1.17 -0.94
17 1019 4nge (A) 31 1025 29.00 0 1 1.32969 -0.44
28 1018 4l3t (A) 44 1024 34.00 0 1 1.31987 -0.2
29 1024 2fge (A) 18 985 30.00 0 1 1.32376 -0.39
844 1020 3ivl (A) 272 450 14.00 0 1 0.322452 -0.51

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127676)

Species Accession Gene Product
Arabidopsis thaliana AT1G49630   presequence protease 2
Arabidopsis thaliana AT3G19170   zinc metalloprotease pitrilysin subfamily A
Babesia bovis BBOV_II004890   peptidase M16 inactive domain containing protein
Candida albicans CaO19.7410   potential metalloendopeptidase similar to S. cerevisiae CYM1
Chlamydia trachomatis CT_824   zinc metalloprotease
Dictyostelium discoideum DDB_G0271506   peptidase M16 family protein
Drosophila melanogaster Dmel_CG3107   CG3107 gene product from transcript CG3107-RB
Echinococcus granulosus EgrG_000503400   presequence protease mitochondrial
Entamoeba histolytica EHI_124430   Zn-dependent peptidase, putative
Echinococcus multilocularis EmuJ_000503400   presequence protease, mitochondrial
Giardia lamblia GL50803_9508   Metalloprotease, insulinase family
Giardia lamblia GL50803_93551   Metalloprotease, insulinase family
Homo sapiens ENSG00000107959   pitrilysin metallopeptidase 1
Leishmania braziliensis LbrM.07.0100   pitrilysin-like metalloprotease,metallo-peptidase, Clan ME, Family M16C
Leishmania donovani LdBPK_070250.1   pitrilysin-like metalloprotease
Leishmania infantum LinJ.07.0250   pitrilysin-like metalloprotease,metallo-peptidase, Clan ME, Family M16C
Leishmania major LmjF.07.0100   pitrilysin-like metalloprotease,metallo-peptidase, Clan ME, Family M16C
Leishmania mexicana LmxM.07.0100   pitrilysin-like metalloprotease,metallo-peptidase, Clan ME, Family M16C
Mus musculus ENSMUSG00000021193   pitrilysin metallepetidase 1
Neospora caninum NCLIV_045460   Mitochondrial presequence protease (EC 3.4.24.-) (Precursor), related
Oryza sativa 4330808   Os02g0761400
Plasmodium berghei PBANKA_1137000   bergheilysin
Plasmodium falciparum PF3D7_1360800   falcilysin
Plasmodium knowlesi PKNH_1110600   falcilysin, putative
Plasmodium vivax PVX_115000   falcilysin, putative
Plasmodium yoelii PY07032   falcilysin-related
Saccharomyces cerevisiae YDR430C   Cym1p
Schistosoma japonicum Sjp_0313060   ko:K06972 pitrilysin metalloproteinase 1, putative
Schistosoma japonicum Sjp_0311050   Presequence protease, mitochondrial precursor, putative
Schistosoma japonicum Sjp_0309370   ko:K06972 Pitrilysin metalloproteinase 1, putative
Schistosoma mansoni Smp_146140   eupitrilysin (M16 family)
Schmidtea mediterranea mk4.001637.03   Presequence protease, mitochondrial
Schmidtea mediterranea mk4.027965.01  
Schmidtea mediterranea mk4.001637.02   Presequence protease, mitochondrial
Schmidtea mediterranea mk4.027965.00  
Schmidtea mediterranea mk4.001637.01   Presequence protease, mitochondrial
Schmidtea mediterranea mk4.002273.04  
Schmidtea mediterranea mk4.027965.02  
Trypanosoma brucei gambiense Tbg972.8.1490   pitrilysin-like metalloprotease, putative,metallo-peptidase, Clan ME, Family M16C, putative
Trypanosoma brucei Tb927.8.1860   pitrilysin-like metalloprotease
Trypanosoma congolense TcIL3000_8_1790   pitrilysin-like metalloprotease, putative
Trypanosoma cruzi TcCLB.511391.200   pitrilysin-like metalloprotease, putative
Toxoplasma gondii TGME49_227948   peptidase M16 inactive domain-containing protein
Treponema pallidum TP0025   hypothetical protein
Theileria parva TP02_0260   falcilysin, putative
Theileria parva TP02_0257   falcilysin, putative
Trichomonas vaginalis TVAG_151080   Clan ME, family M16, insulinase-like metallopeptidase
Trichomonas vaginalis TVAG_484640   Clan ME, family M16, insulinase-like metallopeptidase
Trichomonas vaginalis TVAG_087590   Clan ME, family M16, insulinase-like metallopeptidase
Trichomonas vaginalis TVAG_016860   Clan ME, family M16, insulinase-like metallopeptidase

Essentiality

TcCLB.511391.200 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.1860 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.1860 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.1860 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.1860 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1137000 Plasmodium berghei Essential plasmo
TGME49_227948 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Plasmodium falciparum falcilysin Compounds References
Homo sapiens pitrilysin metallopeptidase 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.0045 0.3163 0

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.511391.200 (Trypanosoma cruzi), pitrilysin-like metalloprotease, putative
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