Detailed view for TcCLB.510997.40

Basic information

TDR Targets ID: 42903
Trypanosoma cruzi, enoyl-CoA hydratase/isomerase family protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.6466 | Length (AA): 276 | MW (Da): 29812 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00378   Enoyl-CoA hydratase/isomerase

Gene Ontology

Mouse over links to read term descriptions.
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
7 274 1wz8 (A) 2 264 29.00 0 1 1.36 -0.99
20 250 2a7k (A) 1 230 26.00 0 1 1.28 -1.51
31 276 1hzd (A) 91 339 45.00 0 1 1.49 -0.74
5 276 3g64 (A) 1 275 28.00 0.00000035 1 1.29711 0.29
21 276 1hzd (A) 81 339 43.00 0 1 1.42754 -0.11
21 276 3kqf (A) 8 262 43.00 0 1 1.47264 -0.4
22 247 2a7k (A) 3 227 27.00 0 1 1.22094 -0.62
23 276 5jbx (A) 6 258 45.00 0.00000015 1 1.46689 -0.26

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_128987)

Species Accession Gene Product
Arabidopsis thaliana AT4G16800   ATP-dependent caseinolytic (Clp) protease/crotonase family protein
Caenorhabditis elegans CELE_F56B3.5   Protein ECH-5
Dictyostelium discoideum DDB_G0289471   enoyl-CoA hydratase/isomerase domain-containing protein
Drosophila melanogaster Dmel_CG8778   CG8778 gene product from transcript CG8778-RA
Homo sapiens ENSG00000148090   AU RNA binding protein/enoyl-CoA hydratase
Homo sapiens ENSG00000121310   enoyl CoA hydratase domain containing 2
Leishmania braziliensis LbrM.34.0400   enoyl-CoA hydratase/isomerase family protein, conserved
Leishmania donovani LdBPK_350360.1   enoyl-CoA hydratase/isomerase family protein, conserved
Leishmania infantum LinJ.35.0360   enoyl-CoA hydratase/isomerase family protein, conserved
Leishmania major LmjF.35.0360   enoyl-CoA hydratase/isomerase family protein, conserved
Leishmania mexicana LmxM.34.0360   enoyl-CoA hydratase/isomerase family protein, conserved
Mus musculus ENSMUSG00000028601   enoyl Coenzyme A hydratase domain containing 2
Mus musculus ENSMUSG00000021460   AU RNA binding protein/enoyl-coenzyme A hydratase
Neospora caninum NCLIV_022740   enoyl-CoA hydratase/isomerase family protein, putative
Oryza sativa 4330183   Os02g0654000
Oryza sativa 4330184   Os02g0654100
Schmidtea mediterranea mk4.005506.00  
Trypanosoma brucei gambiense Tbg972.10.5000   methylglutaconyl-CoA hydratase, mitochondrial precursor, putative
Trypanosoma brucei Tb927.10.4000   methylglutaconyl-CoA hydratase, mitochondrial precursor, putative
Trypanosoma congolense TcIL3000_10_3330   methylglutaconyl-CoA hydratase, mitochondrial precursor, putative
Trypanosoma cruzi TcCLB.510997.40   enoyl-CoA hydratase/isomerase family protein, putative
Trypanosoma cruzi TcCLB.506959.40   enoyl-CoA hydratase/isomerase family protein, putative
Toxoplasma gondii TGME49_202140   enoyl-CoA hydratase/isomerase family protein

Essentiality

TcCLB.510997.40 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.4000 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.4000 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.4000 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.4000 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_202140 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.510997.40 (Trypanosoma cruzi), enoyl-CoA hydratase/isomerase family protein, putative
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