Detailed view for TcCLB.473111.10

Basic information

TDR Targets ID: 43033
Trypanosoma cruzi, lathosterol oxidase, putative

Source Database / ID:  TriTrypDB  GeneDB

pI: 7.7576 | Length (AA): 278 | MW (Da): 32813 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 4

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04116   Fatty acid hydroxylase superfamily

Gene Ontology

Mouse over links to read term descriptions.
GO:0005783   endoplasmic reticulum  
GO:0005506   iron ion binding  
GO:0016491   oxidoreductase activity  
GO:0055114   oxidation reduction  
GO:0008610   lipid biosynthetic process  
GO:0006633   fatty acid biosynthetic process  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 268 1ezv (C) 42 314 12.00 0 0.04 1.12 -0.84
1 165 1q90 (B) 46 213 9.00 0.0000000000016 0.02 0.79 -1.25

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein


Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.


Ortholog group members (OG5_129040)

Species Accession Gene Product
Arabidopsis thaliana AT3G02580   delta(7)-sterol-C5(6)-desaturase 1
Arabidopsis thaliana AT3G02590   putative Delta(7)-sterol-C5(6)-desaturase 2
Candida albicans CaO19.8387   similar to S. cerevisiae ERG3 (YLR056W) sterol C5, 6 desaturase in volved in ergosterol biosynthesis
Candida albicans CaO19.767   similar to S. cerevisiae ERG3 (YLR056W) sterol C5, 6 desaturase in volved in ergosterol biosynthesis
Dictyostelium discoideum DDB_G0279611   sterol desaturase family protein
Homo sapiens 6309   sterol-C5-desaturase
Leishmania braziliensis LbrM.23.1420   lathosterol oxidase-like protein
Leishmania donovani LdBPK_231560.1   lathosterol oxidase-like protein
Leishmania infantum LinJ.23.1560   lathosterol oxidase-like protein
Leishmania major LmjF.23.1300   lathosterol oxidase-like protein
Leishmania mexicana LmxM.23.1300   lathosterol oxidase-like protein
Mus musculus ENSMUSG00000032018   sterol-C5-desaturase (fungal ERG3, delta-5-desaturase) homolog (S. cerevisae)
Oryza sativa 4325687   Os01g0134500
Saccharomyces cerevisiae YLR056W   C-5 sterol desaturase
Trypanosoma brucei gambiense Tbg972.8.2980   lathosterol oxidase, putative
Trypanosoma brucei Tb927.8.3240   lathosterol oxidase, putative
Trypanosoma congolense TcIL3000_8_3280   lathosterol oxidase, putative
Trypanosoma cruzi TcCLB.473111.10   lathosterol oxidase, putative
Trypanosoma cruzi TcCLB.507853.10   lathosterol oxidase, putative


TcCLB.473111.10 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.3240 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.3240 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.3240 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.3240 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site,, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model


Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.473111.10 (Trypanosoma cruzi), lathosterol oxidase, putative
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